Effects of Exopolysaccharide-Producing Probiotic Strains on Experimental Colitis in Rats
Tóm tắt
Inflammatory bowel disease is suggested to result from a dysregulated immune response toward intestinal microflora, which may be restored by probiotic therapy based on the concept of healthy microflora. Ideal probiotic bacteria may be beneficial in inflammatory bowel disease; however, the mechanism of action and the clinical efficacy of probiotic usage are still unclear. In the present study, the effect of exopolysaccharide producing probiotics was evaluated on an experimental colitis model in rats. Colitis was induced by intracolonic administration of acetic acid. Then, rats were treated daily with two probiotic strains, Lactobacillus delbrueckii subsp. bulgaricus B3 strain (exopolysaccharide of 211 mg/l: high-EPS group) or Lactobacillus
delbrueckii subsp. bulgaricus A13 strain (EPS of 27 mg/l: low-EPS group), which were given into the stomach. The non-colitis–fed control group was only treated with high-exopolysaccharide strain. The model-control and control groups were treated only with tap water. Rats were killed after a seven-day treatment period. Disease activity was quantified by use of histologic scores and colonic myeloperoxidase activity, which is a marker of neutrophil infiltration during inflammation. The enhanced inflammatory response was accompanied by a higher level of myeloperoxidase activity in the colitis group. Histologic scores of colonic damage and myeloperoxidase activity were lower in both probiotic-treated groups compared with those of the colitis control group (P < 0.001), although the mentioned scores improved significantly more in the high-EPS group than in the low-EPS group (P < 0.001). Exopolysaccharide-producing probiotics significantly attenuate experimental colitis, which may be mediated by exopolysaccharide in a dose-dependent manner. Therefore, exopolysaccharide-producing probiotics may be a promising therapeutic role in inflammatory bowel disease.
Tài liệu tham khảo
F Shanahan (2001) ArticleTitleInflammatory bowel disease: immunodiagnostics, immunotherapeutics and ecotherapeutics Gastroenterology 120 622–635 Occurrence Handle10.1053/gast.2001.22122 Occurrence Handle1:CAS:528:DC%2BD3MXhvFKntrY%3D Occurrence Handle11179240
RJ Farrell JT LaMont (2002) ArticleTitleMicrobial factors in inflammatory bowel disease Gastroenterol Clin North Am 31 41–62 Occurrence Handle10.1016/S0889-8553(01)00004-8 Occurrence Handle12122743
RB Sartor (1997) ArticleTitlePathogenesis and immune mechanisms of chronic inflammatory bowel diseases Am J Gastroenterol 92 IssueIDSuppl 5S–11S Occurrence Handle1:STN:280:DyaK1c%2FlsFOhtg%3D%3D Occurrence Handle9395346
R Fuller (1989) ArticleTitleProbiotics in man animals J Appl Bacteriol 66 365–378 Occurrence Handle1:STN:280:DyaL1MzislSqsw%3D%3D Occurrence Handle2666378
P Gionchetti C Amadini F Rizzello et al. (2002) ArticleTitleProbiotics-role in inflammatory bowel disease Dig Liver Dis 34 IssueIDSuppl 2 S58–S62 Occurrence Handle12408442 Occurrence Handle10.1016/S1590-8658(02)80166-9
CE McNaught J MacFie (2001) ArticleTitleProbiotics in clinical practice: a critical review of the evidence Nutr Res 21 343–353 Occurrence Handle10.1016/S0271-5317(00)00286-4 Occurrence Handle1:CAS:528:DC%2BD3MXitFajs70%3D
C Fiocchi (1998) ArticleTitleInflammatory bowel disease: etiology and pathogenesis Gastroenterol 115 182–205 Occurrence Handle1:STN:280:DyaK1czitlGjtg%3D%3D Occurrence Handle10.1016/S0016-5085(98)70381-6
CP Tamboli C Caucheteux A Cortot JF Colombel P Desreumaux (2003) ArticleTitleProbiotics in inflammatory bowel disease: a critical review Best Pract Res Clin Gastroenterol 17 805–820 Occurrence Handle10.1016/S1521-6918(03)00076-3 Occurrence Handle14507590
P Marteau P Seksik F Shanahan (2003) ArticleTitleManipulation of the bacterial flora in inflammatory bowel disease Best Pract Res Clin Gastroenterol 17 47–61 Occurrence Handle1:CAS:528:DC%2BD3sXhs1Cht70%3D Occurrence Handle12617882 Occurrence Handle10.1053/bega.2002.0344
SL Gorbach (2002) ArticleTitleProbiotics in the third millennium Dig Liver Dis 34 IssueIDSuppl 2 S2–S7 Occurrence Handle12408431 Occurrence Handle10.1016/S1590-8658(02)80155-4
F Shanahan (2000) ArticleTitleProbiotics in inflammatory bowel disease: is there a scientific rationale? Inflamm Bowel Dis 6 107–115 Occurrence Handle1:STN:280:DC%2BD3cvjsVKqtQ%3D%3D Occurrence Handle10833070
L Vuyst ParticleDe B Degeest (1999) ArticleTitleHeteropolysaccharides from lactic acid bacteria FEMS Microbiol Rev 23 153–177 Occurrence Handle10234843 Occurrence Handle10.1016/S0168-6445(98)00042-4
A Laws Y Gu V Marshall (2001) ArticleTitleBiosynthesis, characterization, and design of bacterial exopolysaccharides from lactic acid bacteria Biotechnol Adv 19 597–625 Occurrence Handle10.1016/S0734-9750(01)00084-2 Occurrence Handle1:CAS:528:DC%2BD38XhvVWrsr0%3D Occurrence Handle14550013
EP Knoshaug JA Ahlgrent JE Trempy (2000) ArticleTitleGrowth associated exopolysaccharide expression in Lactococcuc lactis subspecies cremoris Ropy352 J Dairy Sci 83 633–640 Occurrence Handle1:CAS:528:DC%2BD3cXivFWltr4%3D Occurrence Handle10791777
PJ Looijesteijn L Trapet E Vries ParticleDe T Ebee J Hugenholtz (2001) ArticleTitlePhysiological function of exopolysaccharides produced by Lactococcus lactis Int J Food Microbiol 64 71–80 Occurrence Handle10.1016/S0168-1605(00)00437-2 Occurrence Handle1:CAS:528:DC%2BD3MXhtFaqs7s%3D Occurrence Handle11252513
P Ruas-Madiedo CG Reyes-Gavilan ParticleDe los (2005) ArticleTitleInvited review: methods for the screening, isolation, and characterization of exopolysaccharides produced by lactic acid bacteria J Dairy Sci 88 843–856 Occurrence Handle1:CAS:528:DC%2BD2MXitFygtrg%3D Occurrence Handle15738217 Occurrence Handle10.3168/jds.S0022-0302(05)72750-8
C Wacher-Rodarte M Galvan A Farres F Gallardo VM Marchall M Garcia-Garibay (1993) ArticleTitleYogurt production from reconstituted skim milk powders using different polymer and nonpolymer forming starter cultures J Dairy Res 60 247–254 Occurrence Handle10.1017/S0022029900027564
RI Dave NP Shah (1997) ArticleTitleEffect of level of starter culture on viability of yogurt and probiotic bacteria in yogurts Food Australia 49 164–168
P Ruas Madiedo J Hugenholtz P Zoon (2002) ArticleTitleAn overview of the functionality of exopolysaccharides produced by lactic acid bacteria Int Dairy J 12 163–171 Occurrence Handle1:CAS:528:DC%2BD38XhslSrtLs%3D Occurrence Handle10.1016/S0958-6946(01)00160-1
RM Pigeon EP Cuesta SE Gililliand (2002) ArticleTitleBinding of free bile acids by cells of yogurt starter culture bacteria J Dairy Sci 85 2705–2710 Occurrence Handle1:CAS:528:DC%2BD38XptlagsLg%3D Occurrence Handle12487437 Occurrence Handle10.3168/jds.S0022-0302(02)74357-9
AD Welman IS Maddox (2003) ArticleTitleExopolysaccharides from lactic acid bacteria: perspectives and challenges Trends Biotechnol 21 269–274 Occurrence Handle10.1016/S0167-7799(03)00107-0 Occurrence Handle1:CAS:528:DC%2BD3sXkt1aru74%3D Occurrence Handle12788547
D Roy S Sirois D Vincent (2001) ArticleTitleMolecular discrimination of Lactobacilli used as starter and probiotic cultures by amplified ribosomal DNA restriction analysis Curr Microbiol 42 282–289 Occurrence Handle1:CAS:528:DC%2BD3MXhtVagsbg%3D Occurrence Handle11178730
M Ventura IA Casas L Morelli M Callegari (2000) ArticleTitleRapid amplified ribosomal DNA restriction analysis (ARDRA) identification of Lactobacillus spp. isolated from fecal and vaginal samples Syst Appl Microbiol 23 504–509 Occurrence Handle1:CAS:528:DC%2BD3MXit1WmtLY%3D Occurrence Handle11249020
A Tilsala-Timisjarvi T Alatossava (1993) ArticleTitleEnzymatic amplification of 16 s rRNA gene sequences from dairy Lactobacillus species Milchwissenschaft 48 123–125 Occurrence Handle1:CAS:528:DyaK3sXlslCrt7w%3D
GI Frengova ED Simova DM Beshkova ZI Simov (2000) ArticleTitleProduction and monomer composition of exopolysaccharides by yogurt starter cultures Can J Microbiol 46 123–127 Occurrence Handle10.1139/cjm-46-12-1123
M Dubois KA Gilles JK Hamilton PA Peters F Smith (1956) ArticleTitleColorimetric method for determination of sugars and related substances Anal Chem 28 350–356 Occurrence Handle10.1021/ac60111a017 Occurrence Handle1:CAS:528:DyaG28XjvFynsg%3D%3D
MI Torino MP Taranto F Sesma G Font de Valdez (2001) ArticleTitleHeterofermentative pattern and exopolysaccharide production by Lactobacillus helveticus 15807 in response to environmental pH J Appl Microbiol 91 846–852 Occurrence Handle10.1046/j.1365-2672.2001.01450.x Occurrence Handle1:CAS:528:DC%2BD3MXpt1Wksr4%3D Occurrence Handle11722662
N Kihara SG Fuente ParticleDe la K Fujino T Takahashi TN Pappas CR Mantyh (2003) ArticleTitleVanilloid receptor-1 containing primary sensory neurones mediate dextran sulphate sodium induced colitis in rats Gut 52 713–719 Occurrence Handle10.1136/gut.52.5.713 Occurrence Handle1:CAS:528:DC%2BD3sXjvVeiu7k%3D Occurrence Handle12692058
J Fioramonti V Theodorou L Bueno (2003) ArticleTitleProbiotics: what are they? What are their effects on gut physiology? Best Pract Res Clin Gastroenterol 17 711–724 Occurrence Handle10.1016/S1521-6918(03)00075-1 Occurrence Handle1:CAS:528:DC%2BD3sXnsVWkt7w%3D Occurrence Handle14507583
P Marteau P Seksik F Shanahan (2003) ArticleTitleManipulation of the bacterial flora in inflammatory bowel disease Best Pract Res Clin Gastroenterol 17 47–61 Occurrence Handle1:CAS:528:DC%2BD3sXhs1Cht70%3D Occurrence Handle12617882 Occurrence Handle10.1053/bega.2002.0344
J McCarthy L O'Mahony L O'Callaghan et al. (2003) ArticleTitleDouble blind, placebo controlled trial of two probiotic strains in interleukin 10 knockout mice and mechanistic link with cytokine balance Gut 52 975–980 Occurrence Handle10.1136/gut.52.7.975 Occurrence Handle1:STN:280:DC%2BD3s3psV2jtg%3D%3D Occurrence Handle12801954
O Shibolet F Karmeli R Eliakim et al. (2002) ArticleTitleVariable response to probiotics in two models of experimental colitis in rats Inflamm Bowel Dis 8 399–406 Occurrence Handle12454615 Occurrence Handle10.1097/00054725-200211000-00004
BJ Rembacken AM Snelling PM Hawkey DM Chalmers AT Axon (1999) ArticleTitleNon-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trial Lancet 354 635–639 Occurrence Handle1:STN:280:DyaK1MzpsVGnsw%3D%3D Occurrence Handle10466665 Occurrence Handle10.1016/S0140-6736(98)06343-0
P Gionchetti F Rizzello U Helwig et al. (2003) ArticleTitleProphylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial Gastroenterology 124 1202–1209 Occurrence Handle10.1016/S0016-5085(03)00171-9 Occurrence Handle12730861
RJ Kennedy M Hoper K Deodhar SJ Kirk KR Gardiner (2000) ArticleTitleProbiotic therapy fails to improve gut permeability in a hapten model of colitis Scand J Gastroenterol 35 1266–1271 Occurrence Handle1:CAS:528:DC%2BD3MXot1Gitw%3D%3D Occurrence Handle11199365 Occurrence Handle10.1080/003655200453601
R Temmerman B Pot G Huys J Swings (2003) ArticleTitleIdentification and antibiotic susceptibility of bacterial isolates from probiotic products Int J Food Microbiol 81 1–10 Occurrence Handle10.1016/S0168-1605(02)00162-9 Occurrence Handle1:CAS:528:DC%2BD38Xotlyqur0%3D Occurrence Handle12423913
X Guo WP Wang JK Ko (1990) ArticleTitleInvolvement of neutrophils and free radicals in the potentiating effects of passive cigarette smoking on inflammatory bowel disease in rats Gastroenterology 117 884–892 Occurrence Handle10.1016/S0016-5085(99)70347-1
U Burner W Jantschko C Obinger (1999) ArticleTitleKinetics of oxidation of aliphatic and aromatic thiols by myeloperoxidase compounds I and II FEBS Lett 443 290–296 Occurrence Handle10.1016/S0014-5793(98)01727-X Occurrence Handle1:CAS:528:DyaK1MXht1SgtLg%3D Occurrence Handle10025950
AJ Kettle CC Winterbourn (1991) ArticleTitleMechanism of inhibition of myeloperoxidase by anti-inflammatory drugs Biochem Pharmacol 41 1485–1492 Occurrence Handle10.1016/0006-2952(91)90565-M Occurrence Handle1:CAS:528:DyaK3MXkt1Wrsbs%3D Occurrence Handle1850278
MB Grisham JN Beniot DN Granger (1990) Assessment of leukocyte involvement during ischemia and reperfusion on the intestine L Packer AE Glazer (Eds) Methods enzymol oxygen radicals in biological systems Academic Press San Diego 729–741
AL Servin MH Coconnier (2003) ArticleTitleAdhesion of probiotic strains to the intestinal mucosa and interaction with pathogens Best Pract Res Clin Gastroenterol 17 741–754 Occurrence Handle10.1016/S1521-6918(03)00052-0 Occurrence Handle1:CAS:528:DC%2BD3sXnsVWkt7o%3D Occurrence Handle14507585
HS Gill (2003) ArticleTitleProbiotics to enhance anti-infective defenses in the gastrointestinal tract Best Pract Res Clin Gastroenterol 17 755–773 Occurrence Handle10.1016/S1521-6918(03)00074-X Occurrence Handle1:CAS:528:DC%2BD3sXnsVWkt7s%3D Occurrence Handle14507586
MF Neurath C Becker K Barbulescu (1998) ArticleTitleRole of NF-kappaB in immune and inflammatory responses in the gut Gut 43 856–860 Occurrence Handle1:CAS:528:DyaK1cXotVagt7o%3D Occurrence Handle9824616 Occurrence Handle10.1136/gut.43.6.856
AS Neish AT Gewirtz H Zeng et al. (2001) ArticleTitleProkaryotic regulation of epithelial responses by inhibition of IkappaB-alpha ubiquitination Science 289 1560–1563 Occurrence Handle10.1126/science.289.5484.1560
G Reid J Howard BS Gan (2001) ArticleTitleCan bacterial interference prevent infection? Trends Microbiol 9 424–428 Occurrence Handle10.1016/S0966-842X(01)02132-1 Occurrence Handle1:CAS:528:DC%2BD3MXmvVajsLo%3D Occurrence Handle11553454
IW Sutherland (2001) ArticleTitleMicrobial polysaccharides from gram-negative bacteria Int Dairy J 11 663–674 Occurrence Handle10.1016/S0958-6946(01)00112-1 Occurrence Handle1:CAS:528:DC%2BD3MXnsF2jsb4%3D
L Jolly F Stingele (2001) ArticleTitleMolecular organization and functionality of exopolysaccharide gene clusters in lactic acid bacteria Int Dairy J 11 733–745 Occurrence Handle10.1016/S0958-6946(01)00117-0 Occurrence Handle1:CAS:528:DC%2BD3MXnsF2jsbs%3D
S Videla J Vilaseca M Antolin et al. (2001) ArticleTitleDietary inulin improves distal colitis induced by dextran sodium sulfate in the rat Am J Gastroenterol 96 1486–1493 Occurrence Handle10.1111/j.1572-0241.2001.03802.x Occurrence Handle1:CAS:528:DC%2BD3MXktlyisrs%3D Occurrence Handle11374687
WA Walker LC Duffy (1998) ArticleTitleDiet and bacterial colonization: role of probiotics and prebiotics J Nutr Biochem 9 668–675 Occurrence Handle1:CAS:528:DyaK1cXnvFKgtLs%3D Occurrence Handle10.1016/S0955-2863(98)00058-8
KM Tuohy HM Probert CW Smejkal GR Gibson (2003) ArticleTitleUsing probiotics and prebiotics to improve gut health Drug Discov Today 8 692–700 Occurrence Handle10.1016/S1359-6446(03)02746-6 Occurrence Handle12927512
AY Tamime (2002) ArticleTitleFermented milks: a historical food with modern applications—a review Eur J Clin Nutr 56 IssueIDSuppl 4 S2–S15 Occurrence Handle1:CAS:528:DC%2BD3sXitVCktb4%3D Occurrence Handle12556941 Occurrence Handle10.1038/sj.ejcn.1601657
P Marteau F Shanahan (2003) ArticleTitleBasic aspects and pharmacology of probiotics: an overview of pharmacokinetics, mechanisms of action and side-effects Best Pract Res Clin Gastroenterol 17 725–740 Occurrence Handle1:CAS:528:DC%2BD3sXnsVWkt70%3D Occurrence Handle14507584 Occurrence Handle10.1016/S1521-6918(03)00055-6
AR Jurjus NN Khoury JM Reimund (2004) ArticleTitleAnimal models of inflammatory bowel disease J Pharmacol Toxicol Methods 50 81–92 Occurrence Handle10.1016/j.vascn.2003.12.002 Occurrence Handle1:CAS:528:DC%2BD2cXnvV2ms7c%3D Occurrence Handle15385082