Application of a new TLC chemical method for detection of cyclopeptides in plants
Tóm tắt
Cyclopeptides have been investigated phytochemically less often because until now there has not been a special chemical method to detect them. Since we found cyclopeptides inPseudostellaria heterophylla (Caryophyllaceae) in 1991, we have gradually established a special chemical detection method for detecting cyclopeptides in plants, which induces a new thin layer chromatography (TLC) protosite reaction with ninhydrin reagent. With this method, our group isolated and determined 73 cyclopeptides from 17 plants which belong to 5 families and 14 genuses, they are from dicyclopeptides to undecacyclopeptides, including 68 new ones, and were determined based on spectral, chemical and enzymic methods, especially 2D NMR and FAB-MS. Meantime, with this method cyclopeptides can be distinguished from peptidic amides based on their behaviour in TLC.
Tài liệu tham khảo
Tan, N. H., Zhou, J., Zhao, S. X., Progress in the chemistry of cyclopeptides in plants, Acta Pharmaceutica Sinica (in Chinese), 1997, 32(5): 388.
Tan, N. H., Zhou, J., Chen, C. X. el al., Cyclopeptides from the roots ofPseudostellario heterophylla, Phytochemistry, 1993, 32(5): 1327.
Hao, X. Y., Tan, N. H., Zhou, J., Constituents ofGtrstrotdia elatar in Guizhou, Acta Botanica Yunnanica (in Chinese), 2000, 22(1): XI.
Zou, C., Zhang, Y. P., Zhao, B. T., et al., A bioactive amide from roots ofAster tartaricus, Acta Botanica Yunnanica (in Chinese), 1999, 21: 121.
Li, C. M., Tan, N. H., Mu, Q. et al., Cyclopeptides from the seeds ofAnnona glabra, Phytochemistry, 1998, 47(7): 1293.
Li, C. M., Tan, N. H., Zhen, H. L. et al., Cyclopeptides from the seeds ofAnnona glabra, Phytochemistry, 1999, 50(6): 1047.
Li, C. M., Tan, N. H., Lu, Y. P. et al., Annomuricatin A, a new cyclopeptide from the seeds ofAnnona muricata, Acta Botanica Yunnanica, 1995, 17(1): 459.
Li, C. M., Tan, N. H., Zhen, H. L. et al., Cyclopeptide from the seedsof Annona muricata, Phytochemistry, 1998, 48(3): 555.
Li, C. M., Tan, N. H., Mu, Q. et al., Cyclopeptide from the seeds ofAnnona squamosa, Phytochemistry, 1997, 45(3): 521.
Wang, Y. C., Tan, N. H., Zhou, J. el al., Cyclopeptides fromDianthus superbus, Phytochemistry, 1998, 49(5): 1453.
Ding, Z. T., Wang, Y. C., Zhou, J. et al., Cyclopeptides fromPolycarpon prostraturn, Chinese Chemical Letters, 1999, 10(12): 1037.
Tan, N. H., Zhou, J., Heterophyllin, C., a new cyclopeptide fromPseudostellaria heterophylla, Acta Botanica Yunnanica, 1995, 17(1): 60.
Zou, C., Hao, X. J., Zhou, J., Antitumor glycocyclohexapeptide fromRubia yunnanensis, Acta Botanica Yunnanica, 1993, 15(4): 399.
He, M., Zou, C., Hao, X. J. et al., A new antitumor cyclic hexapeptide glycoside fromRubia yunnanensis, Acta Botanica Yunnanica, 1993, 15(4): 408.
He, M., Zou, C., Hao, X. J. et al., New antitumor glycocyclohexapeptide fromRubia yunnanensis, Chinese Chemical Letters, 1993, 4: 1065.
Wang, Y. C., Zhou, J., Tan, N. H. et al., Cyclic dipeptides fromSchizandra chinensis and their syntheses, Acta Pharmaceutica Sinica (in Chinese), 1999, 34(1): 19.
Zhang, R. P., Zou, C., He, Y. N. et al., Three new cyclopeptides fromSilene szechuensis, Acta Botanica Yunnanica, 1997, 19(3): 304.
Zhao, Y. R., Zhou, J., Wang, X. K. et al., Three cyclopeptides fromStellaria delavayi, Phytochemistry, 1997, 46(4): 709.
Zhao, Y. R., Zhou, J., Wang, X. K. et al., Stelladelin D, a new cyclic undecapeptide fromStellaria delavayi, Chinese Chemical Letters, 1996, 7: 237.
Zhao, Y. R., Zhou, J., Wang, X. K. et al., Revision of the structure of stellarin A, Acta Botanica Yunnanica, 1995, 17(3): 345.
Zhao, Y. R., Zhou, J., Wang, X. K. et al., Cyclopeptides fromStellarirl yunnanensis, Phytochemistry, 1995, 40(5): 1453.
Zhao, Y. R., Wang, X. K., Zhao, T. F. et al., Two new cyclopeptides fromStellaria yunnanensis, Chinese Journal of Chemistry, 1995, 13(3): 267.
Zhao, Y. R., Wang, X. K., Zhou, J. et al., Stellarins F and G, two new cyclopeptides fromStellaria yunnanensis, Chinese Journal of Chemistry, 1995, 13(6): 552.
Zhao, Y. R., Zhou, J., Wang, X. K. el al., The structure of stellarin H, Acta Botanica Yunnanica, 1995, 17(4): 463.
Zhang, Y. P., Zou, C., Tan, N. H. et al., Cyclopeptides fromVaccaricz segetalis, Acta Botanica Yunnanica, 1998, 20(1): 105.