The surface membrane proteins of the WI-38 fibroblast in relation to transformation and aging
Tóm tắt
The urea extractable cell surface proteins of WI-38 human embryonic fibroblasts and SV-40 transformed WI-38 fibroblasts were examined. Electrophoretic analysis on 5.6% SDS polyacrylamide gels revealed 10–15 discrete protein bands. Three of these bands, of molecular weight 50,000, 45,000 and 30,000 daltons, were found to be present in increased amounts in transformed fibroblasts. The expression of these proteins decreased with increasing culture age. Thus a direct relationship exists between the expression of these proteins and the proliferative state of the culture.
Tài liệu tham khảo
Hayflick, L., and Moorehead, P. S.: The serial cultivation of human diploid cell strains. Exp. Cell. Res., 25:585–621, 1961.
Martin, G. M.: Cellular aging-clonal senescence. Am. J. Path., 89:484–511, 1977.
Cristofalo, V. J.: Animal cell cultures as a model system for studying aging. Adv. Geront. Res., 4: 45–79, 1972.
Inbar, M., Ben Basset, H., and Sachs, L.: Local-isation of amino acid and carbohydrate transport sites in the surface membrane of normal and transformed human cells. J. Memb. Biol., 6:195–209, 1971.
Iselbacher, K. J.: Increased uptake of amino acid and 2-deoxy-d-glucose by virus transformed cells in culture. Proc. Nat. Acad. Scio U.S.A., 69:585–589, 1972.
Whittenberger, B. and Glaser, L.: Inihibition of DNA synthesis in cultures of 3T3_cells by isolated surface membranes. Proc. Nat. Acad. Sci. U.S.A., 74: 2251–2255, 1977.
Yamada, K. M., Yamada, S. A., and Pastan, I.: Cell surface protein partially restores morphology, adhesiveness and contact inhibition of movement. Proc. Nat. Acad. Sci. U.S.A., 73:1217–1221.
Absher, P. M., Absher, R. G., and Barnes, W. D.: Genealogies of clones of diploid fibroblasts. Exp. Cell Res., 88:95–104, 1974.
Kahan, B. D., Rutzky, L. P., Kahan, V. A., Oyasu, R., Wiseman, F., and LeGrue, S.: Cell surface changes associated with malignant transformation of bladder epithelium in vitro. Cancer Res., 37:2866–2871, 1977.
Yamada, K. M., and Weston, J. A.: Isolation of a major cell surface glycoprotein from fibroblasts. Proc. Nat. Acad. Sci. U.S.A., 71:3492–3496, 1974.
Lowry, O. H., Rosenbrough, A., Lewis, F., and Randall, R. J.: Protein measurement with the folin reagent. J. Biol. Chem., 193:265–275, 1951.
Fairbanks, G., Steck, T. L., and Wallach, D. F. H.: Electrophoretic analyses of the major polypeptides of the human erythrocyte membrane. Biochem., 10:2606–2617, 1971.