Optimization of upcyte® human hepatocytes for the in vitro micronucleus assay

Astrid Nörenberg1, Stefan Heinz1, Katharina Scheller1, Nicola J. Hewitt1, Joris Braspenning1, Michael Ott2
1Medicyte GmbH, Heidelberg, Germany
2MHH, Medizinische Hochschule Hannover, Germany

Tài liệu tham khảo

Kirkland, 2005, Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. I. Sensitivity, specificity and relative predictivity, Mutat. Res., 584, 1, 10.1016/j.mrgentox.2005.02.004 EU, 2003, EC – Directive 2003/15/EC of the European Parliament and of the Council of 27 February 2003 amending Council Directive 76/768/EEC on the approximation of the laws of the Member States relating to cosmetic products, Official J., L66, 26 European Commission, 2006. Regulation (EC) No. 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACh), establishing a European Chemicals Agency, amending Directive 1999/45/EC and repealing Council Regulation (EEC) No. 793/93 and Commission Regulation (EC) No. 1488/94 as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC, http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX: 2006R1907:EN:NOT. Fowler, 2012, Reduction of misleading (“false”) positive results in mammalian cell genotoxicity assays. I. Choice of cell type, Mutat. Res., 742, 11, 10.1016/j.mrgentox.2011.10.014 Fowler, 2012, Reduction of misleading (“false”) positive results in mammalian cell genotoxicity assays. II. Importance of accurate toxicity measurement, Mutat. Res., 747, 104, 10.1016/j.mrgentox.2012.04.013 Pfuhler, 2009, Reduction of use of animals in regulatory genotoxicity testing: identification and implementation opportunities – report from an ECVAM workshop, Mutat. Res., 680, 31, 10.1016/j.mrgentox.2009.09.002 Kirkland, 2007, How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary follow-up animal tests: report of an ECVAM workshop, Mutat. Res., 628, 31, 10.1016/j.mrgentox.2006.11.008 Burkard, 2012, Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays, Xenobiotica, 42, 939, 10.3109/00498254.2012.675093 Nörenberg, 2010 Bryce, 2007, In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity, Mutat. Res., 630, 78, 10.1016/j.mrgentox.2007.03.002 Dertinger, 2011, Flow cytometric scoring of micronucleated erythrocytes: an efficient platform for assessing in vivo cytogenetic damage, Mutagenesis, 26, 139, 10.1093/mutage/geq055 Torous, 2006, Performance of flow cytometric analysis for the micronucleus assay – a reconstruction model using serial dilutions of malaria-infected cells with normal mouse peripheral blood, Mutagenesis, 21, 11, 10.1093/mutage/gei053 Kirkland, 2008, Recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests: a follow-up to an ECVAM workshop, Mutat. Res., 653, 99, 10.1016/j.mrgentox.2008.03.008 Malich, 1997, The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines, Toxicology, 124, 179, 10.1016/S0300-483X(97)00151-0 Aardema, 2013, Evaluation of chemicals requiring metabolic activation in the epiderm™ 3D human reconstructed skin micronucleus (RSMN) assay, Mutat. Res., 750, 40, 10.1016/j.mrgentox.2012.08.009 Andrieux, 2007, GATA-1 is essential in EGF-mediated induction of nucleotide excision repair activity and ERCC1 expression through ERK2 in human hepatoma cells, Cancer Res., 67, 2114, 10.1158/0008-5472.CAN-06-3821 Dierssen, 2008, Molecular dissection of gp130-dependent pathways in hepatocytes during liver regeneration, J. Biol. Chem., 283, 9886, 10.1074/jbc.M705483200 Streetz, 2003, Lack of gp130 expression in hepatocytes promotes liver injury, Gastroenterology, 125, 532, 10.1016/S0016-5085(03)00901-6 Banič, 2011, DMSO modulates the pathway of apoptosis triggering, Cell. Mol. Biol. Lett., 16, 328, 10.2478/s11658-011-0007-y Kashino, 2010, An alternative mechanism for radioprotection by dimethylsulfoxide; possible facilitation of DNA double-strand break repair, J. Radiat. Res., 51, 733, 10.1269/jrr.09106 LeCluyse, 2000, Expression and regulation of cytochrome P450 enzymes in primary cultures of human hepatocytes, J. Biochem. Mol. Toxicol., 14, 177, 10.1002/(SICI)1099-0461(2000)14:4<177::AID-JBT1>3.0.CO;2-4 Peace, 1999, Spontaneous micronucleus frequency and age: what are normal values?, Mutat. Res., 425, 225, 10.1016/S0027-5107(99)00030-5 Canonero, 1997, Testing of p-dichlorobenzene and hexachlorobenzene for their ability to induce DNA damage and micronucleus formation in primary cultures of rat and human hepatocytes, Mutagenesis, 12, 35, 10.1093/mutage/12.1.35 Martelli, 2002, DNA damage in tissues of rat treated with potassium canrenoate, Toxicology, 171, 95, 10.1016/S0300-483X(01)00562-5 Hayflick, 1998, A brief history of the mortality and immortality of cultured cells, Keio J. Med., 47, 174, 10.2302/kjm.47.174