Immunolocalization of Melatonin and Follicle‐Stimulating Hormone Receptors in Caprine Ovaries and their Effects During in vitro Development of Isolated Pre‐Antral Follicles

Reproduction in Domestic Animals - Tập 48 Số 6 - Trang 1025-1033 - 2013
VRP Barros1, A.Y.P. Cavalcante1, T.J.S. Macedo1, RS Barberino1, T.L.B.G. Lins1, B.B. Gouveia1, Vanúzia Gonçalves Menezes1, Mário Adriano Ávila Queiróz2, Valdevane Rocha Araújo3, RC Palheta4, MCP Leite5, Maria Helena Tavares de Matos1
1Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil
2Laboratory of Bromatology and Animal Nutrition,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
3Faculty of Veterinary Medicine, LAMOFOPA, State University of Ceara, Fortaleza, CE, Brazil
4Laboratory of Physiology, Federal University of San Francisco Valley, Petrolina, PE, Brazil
5Center of Agrarian, Ambiental and Biological Sciences, Federal University of Reconcavo of Bahia, Cruz das Almas, BA, Brazil

Tóm tắt

ContentsThe expression of melatonin type 1 (MT1) and FSH (FSHR) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α‐MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR. Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles.

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Reproduction in Domestic Animals

Tập 48 Số 6

1025-1033

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