Optogenetic Control of Fly Optomotor Responses

Journal of Neuroscience - Tập 33 Số 34 - Trang 13927-13934 - 2013
Väinö Haikala1, Maximilian Joesch1, Alexander Borst1, Alex S. Mauss1
1Max Planck Institute of Neurobiology, 82152 Martinsried, Germany

Tóm tắt

When confronted with a large-field stimulus rotating around the vertical body axis, flies display a following behavior called “optomotor response.” As neural control elements, the large tangential horizontal system (HS) cells of the lobula plate have been prime candidates for long. Here, we applied optogenetic stimulation of HS cells to evaluate their behavioral role inDrosophila. To minimize interference of the optical activation of channelrhodopsin-2 with the visual perception of the flies, we used a bistable variant called ChR2-C128S. By applying pulses of blue and yellow light, we first demonstrate electrophysiologically that lobula plate tangential cells can be activated and deactivated repeatedly with no evident change in depolarization strength over trials. We next show that selective optogenetic activation of HS cells elicits robust yaw head movements and yaw turning responses in fixed and tethered flying flies, respectively.

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Journal of Neuroscience

Tập 33 Số 34

13927-13934

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