ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton
Tóm tắt
In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat (ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains (mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat (SSR) in phytoplankton genomes, especially in Pseudo-nitzschia, and show their usefulness to cluster intra and inter species. ISSR markers were found to be good markers for genetic characterization and diversity study and led to consider them as new tools for the survey of phytoplankton.
Tài liệu tham khảo
Adachi M, Sako Y, Ishida Y (1997) Analysis of Gymnodinium cate-natum (Dinophyceae) using sequences of the 5.8S RDNA-ITS regions and random amplified polymorphic DNA. Fish. Sci. 63: 701–707.
Bolch CJS, Blackburn SI, Hallegraeff GM, Vaillancourt RE (1999) Genetic variation among strains of the toxic dinoflagellate Gymnodinium catanatum (Dinophyceae). J. Phycol. 35: 359–367.
Bornet B, Branchard M (2001) Nonanchored inter simple se-quence repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Mol. Biol. Rep. 19: 209–215.
Chesnick JM, Morden CW, Schmieg AM (1996) Identity of the en-dosymbiont of Peridinium foliaceum (Pyrrophyta): Analysis of the rbcLS operon. J. Phycol. 32: 850–857.
Galvan MZ, Bornet B, Balatti PA, Branchard M (2003) Inter simple sequence repeat (ISSR) markers as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica 132: 297–301.
Guillard RRL, Ryther JH (1962) Studies on marine planktonic di-atoms. 1. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gram. Can. J. Microbiol. 8: 229–239.
Guillou L, Nézan E, Cueff V, Erard-Le Denn E, Cambon-Bonavita MA, Gentien P, Barbier G (2002) Genetic diversity and molec-ular detection of three toxic dinoflagellate genera (Alexandrium, Dinophysis, and Karenia) from French coasts. Protist 153: 223–238.
Jaccard P (1901) Etude de la distribution florale dans une portion des Alpes et du Jura. Bull. Soc. Vaudoise Sci. Nat. 37: 547–579.
Lundholm N, Daugbjerg N, Moestrup O (2002) Phylogeny of the Bacillariacea with emphasis on the genus Pseudo-nitzschia (Bacillariophyceae) based on partial LSU rDNA. Eur. J. Phycol. 37: 115–134.
Murayama-Kayano E, Yoshimatsu S, Kayano T, Nishio T, Ueda H, Nagamune T (1998) Application of the random amplified poly-morphic DNA(RAPD) technique to distinguishing species of the red tide phytoplankton Chattonella (Raphydophyceae). J. Ferm. Bioengng. 85: 343–345.
Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in com-parison to RFLP and RAPD markers. Theor. Appl. Genet. 94: 597–602.
Oldach DW, Delwiche CF, Jakobsen KS, Tengs T, Brown EG, Kemp-ton JW, Schaefer EF, Bowers HA, Glasgow HB, Burkholder JM, Steidinger KA, Rublee PA (2000) Heteroduplex mobility assay-guided sequences discovery: Elucidation of the small sub-unit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools. Proc. Natl. Acad. Sci. USA 97: 4303–4308.
Provasoli L (1968) Media and prospects for the cultivation of marine algae. In Watanabe A, Hattori A (eds), Cultures and Collections of Algae. Proc. U.S.-Japan Conf. Hakone, Japan, September 1966. Publ. by the Jap. Soc. Plant Physiol., pp. 63–75.
Rohlf M (1998) NTSYS-pc, Numeral Taxonomy and Multivariate Analysis System, Version 2.0. Department of Ecology and Evo-lution, State University of New York.
Scholin CA, Herzog M, Sogin M, Anderson DM (1994) Identifi-cation of group-and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragments of the LSU rRNA gene. J. Phycol. 30: 999–1011.
Scholin CA, Marin R, Miller PE, Doucette GJ, Powell CL, Haydock P, Howard J, Ray J (1999) DNAprobes and a receptor-binding as-say for detection of Pseudo-nitzschia (Bacillariophyceae) species and domoic acid activity in cultured and natural samples. J. Phycol. 35: 1356–1367.
Takishita K, Uchida A (1999) Molecular cloning and nucleotide se-quence analysis of psbA from dinoflagellates: Origin of the di-noflagellate plastid. Phycol. Res. 47: 207–216.
Uribe PC, Suarez-Isla BA, Espejo RT (1999) Ribosomal RNA het-erogeneity and identification of toxic dinoflagellate cultures by heteroduplex mobility assay. J. Phycol. 35: 884–888.
Walsh D, Reeves RA, Saul DJ, Gray RD, MacKenzie L, Bergquist D, Bergquist PL (1998) Heterogeneity of SSU and LSU rDNA sequences of Alexandrium species. Biochem. Syst. Ecol. 26: 495–509.
Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain re-action amplification. Genomics 20: 176–183.