New techniques of demonstrating lymph vessels in skin biopsy specimens and intact skin with the scanning electron microscope

Springer Science and Business Media LLC - Tập 279 - Trang 327-334 - 1987
D. Berens v. Rautenfeld1, D. Lubach2, B. Wenzel-Hora3, J. Klanke1, C. Hunneshagen1
1Centre for Anatomy, Medizinische Hochschule Hannover, Hannover
2Department of Dermatology (Hautklinik Linden), Medizinische Hochschule Hannover, Hannover
3Schering AG, Berlin

Tóm tắt

Two new methods now allow the systematic examination of the morphology and micropathology of the initial lymphatics (lymph sinuses and precollectors) in human skin biopsy specimens under the scanning electron microscope. Pertinent studies and findings in the intact skin of anesthetized pigs demonstrate the standard quality of the biopsy preparations. The following are new findings made with these methods: in the skin of the human head, both the lymph sinuses and the precollectors extend as far as the epidermis. The opening apparatus of these vessels consists not only of anchoring filaments and interendothelial openings, but also of endothelial bridges and trabeculae. The interendothelial openings can be present in three types of transformation (Type I–III). As part of these studies, an attempt was made to standardize the lymphological nomenclature so that it would be acceptable to both anatomists and clinicians.

Tài liệu tham khảo

Bartels P (1909) Das Lymphgefäß. G Fischer, Jena Berens v. Rautenfeld D, Wenzel-Hora B, Budras K-D, Henschel E (1983) Morphologische Untersuchungen des Lymphdrainageweges nach Applikation von Intravitalfarbstoffen und Röntgenkontrastmitteln. Verh Anat Ges 77:533–536 Berens v. Rautenfeld D, Wenzel-Hora B (1985) The nomenclature of the initial lymphatics from a comparative anatomical point of view. In: Casley-Smith JR, Piller NB (eds) Progress in lymphology. Proceedings Xth international congress lymphology, Adelaide. University of Adelaide Press, Adelaide, pp 17–19 Berens v. Rautenfeld D, Castenholz A (1987) Neues zur Form und Funktion der interendothelialen Öffnungen und Ankerfilamente. Verh Anat Ges (Lübeck) 81 (in press) Bollinger A, Jäger K, Sgier F, Seglias J (1981) Fluorescence microlymphography. Circulation 64:1195–1200 Browning J, Casley-Smith JR (1981) Tissue channel morphology in octopus. Cell Tiss Res 215:153–170 Casley-Smith JR (1980) The fine structure and functioning of tissue channels and lymphatics. Lymphology 12:177–183 Casley-Smith JR (1983) The structure and functioning of the blood vessels, interstitial tissues, and lymphatics. In: Földi M, Casley-Smith JR (eds) Lymphangiology. Schattauer, Stuttgart New York, pp 28–164 Castenholz A (1984a) Morphological characteristics of initial lymphatics in the tongue as shown by scanning electron microscopy. Scan Electron Microsc 111:1143–1152 Castenholz A (1984b) Strukturbild und Wirkungsweise der initialen Lymphbahn. Lymphology 8:55–64 Castenholz A (1985) REM-Darstellung von interstitiellen Raumsystemen und Lymphbahnen mit der Kunststoffinjektion. Mikroskopie 2:[Suppl] 16–21 Daroczy J (1984) New structural details of dermal lymphatic valve and its functional interpretation. Lymphology 17:54–60 Forbes G (1938) Lymphatics of the akin, with a note on lymphatic watershed areas. J Anat 72:399–410 Frohmann V (1833) Memoire sur les vaisseaux lymphatiques de la peau, des membranes muqueuses, séreuses, du tissu nerveux et musculaire. J Desoer, Liége Hammersen F, Gräfe G (1984) Anordnung, Bau und Funktion des Lymphgefäßsystems unter besonderer Berücksichtigung der terminalen Lymphbahnen. Mikrozirkulation 5 (Deutsche Abbott GmbH) Hauck G (1980) Der Bindegewebsraum aus der Sicht der Lymphologie. Folia Angiolog 28:200–202 His W (1863) Über das Epithel der Lymphgefässwurzehn und die von Recklinghausen'schen Saftkanälchen. Z Wiss Zool 13:455–473 Hudack SS, McMaster PD (1933) The lymphatic participation in human cutaneous phenomena. J Exp Med 57/II: 751–774 Isenring G, Franzeck UK, Bollinger A (1982) Fluoreszenz-Mikrolymphographie am medialen Malleolus bei Gesunden und Patienten mit primären Lymphoedemen. Schweiz Med Wochenschr 112:225–231 Kinmonth JB (1982) The lymphatics, 2nd edn. Arnold, London Konitz H, Berens v. Rautenfeld D, Klanke J, Wenzel-Hora B (1985) REM-Darstellung der initialen Lymphstrombahn durch indirekte Applikation von Mercox oder Glutaraldehyd. Beitr elektronenmikroskop Direktabb Oberfl 18:249–256 Kubik ST (1980) Drainagemöglichkeiten der Lymphterritorien nach Verletzung peripherer Kollektoren und nach Lymphadenektomie. Folia Angiolog 28:228–237 Kubik ST, Wirth W (1980) Histology, anatomy and lymphographic appearance. In: Viamonte M, Rüttimann A (eds) Allas of lymphography. Thieme, Stuttgart New York, pp 1–17 Kubik ST, Manestar M (1985) Anatomy of lymph capillaries and pre-collectors of the skin. In: Bollinger A, Partsch H, Wolfe JHN (eds) The initial lymphatics. Thieme, Stuttgart New York, pp 66–74 Leak LV (1971) Studies on the permeability of lymphatic capillaries. J Cell Biol 50:300–323 Leak LV (1976) The stucture of lymphatic capillaries in lymph formation. Fed Proc 35/8:1863–1871 Leak LV, Burke JF (1966) Fine structure of the lymphatic capillary and the adjoining connective tissue area. Am J Anat 118:785–809 Leak LV, Burke JF (1968) Ultrastructural studies on the lymphatic anchoring filaments. J Cell Biol 36:129–149 Leu HJ (1985) Patho-anatomical findings in the initial lymphatics. In: Bollinger A, Partsch H, Wolfe JHN (eds) The initial lymphatics. Thieme, Stuttgart New York, pp 84–91 Maler F, Boss CH, Saner H (1985) Mikrolymphography with FITC human albumin. In: Bollinger A, Partsch H, Wolfe JHN (eds) The initial lymphatics. Thieme, Stuttgart New York, pp 106–109 Moe R. Electron microscopic morphology of lymphatic sinuses. Anat Rec 136:p 245 Müller RP, Wenzel-Hora BI, Addicks HW (1985) Erste Mitteilung über die indirekte Lymphographie mit Iotasul im Kopf-Hals-Bereich. Fortschr Röntgenstr 142 (2):218–221 Neumann I (1873) Zur Kenntnis der Lymphgefäße der Haut des Menschen und der Säugetiere. Braumüller, Wien Nomina anatomica 5th edn (1983) Williams and Wilkins, Baltimore London Nomina histological 2nd edn (1983) Williams and Wilkins, Baltimore London Partsch H, Wenzel-Hora B, Urbanek A (1983) Differential diagnosis of lymphedema after indirect lymphography with iotasul. Lymphology 16 (1):12–18 Pullinger ED, Florey HW (1935) Some observations on the structure and function of lymphatics: their behaviour in local edema. Br J Exp Pathol 16:49–61 Shdanow DA (1966) On senile changes in lymphatic capillaries and vessels. J Car Surg 7:108–116 Teichmann L (1861) Das Saugadersystem vom anatomischen Standpunkte. W. Engelmann, Leipzig Unna P (1908) Untersuchungen über die Lymph- und Blutgefäße der äusseren Haut mit besonderer Berücksichtigung der Haarfollikel. Arch Mikrosk Anat 72:161–208 Wenzel-Hora BI, Kalbas B, Siefert, HM, Arndt JD, Schlösser HW, Huth F (1981) Iotasul, a water soluble (nonoily) contrast medium for direct and indirect lymphography. Radiological and morphological investigations in dogs. Lymphology 14:101–112 Yamao O (1932) Feinere Verteilung der Lymphgefässe in der Haut des Menschen. Folia Anat Jap 10:505–513