Cell density-dependent membrane distribution of ganglioside GM3 in melanoma cells

Cellular and Molecular Life Sciences - Tập 80 - Trang 1-15 - 2023
Motohide Murate1,2,3, Noriko Yokoyama4, Nario Tomishige1,2,3, Ludovic Richert2, Nicolas Humbert2, Brigitte Pollet2, Asami Makino1,5, Nozomu Kono6, Laura Mauri7, Junken Aoki6, Yasushi Sako3, Sandro Sonnino7, Naoko Komura8, Hiromune Ando8, Mika K. Kaneko9, Yukinari Kato9, Kei-ichiro Inamori10, Jin-ichi Inokuchi10,11, Yves Mély2, Kazuhisa Iwabuchi4, Toshihide Kobayashi1,2,3
1Lipid Biology Laboratory, RIKEN, Wako, Japan
2Laboratoire de Bioimagerie et Pathologies, UMR 7021, CNRS, Faculté de Pharmacie, Université de Strasbourg, Illkirch, France
3Cellular Informatics Laboratory, RIKEN CPR, Wako, Japan
4Institute for Environmental and Gender-Specific Medicine, Graduate School of Medicine, Juntendo University, Urayasu, Japan
5Molecular Physiology Laboratory, RIKEN CPR, Wako, Japan
6Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-Ku, Japan
7Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy
8Institute for Glyco-Core Research, Gifu University, Gifu, Japan
9Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan
10Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan
11Forefront Research Center, Graduate School of Science, Osaka University, Toyonaka, Japan

Tóm tắt

Monosialoganglioside GM3 is the simplest ganglioside involved in various cellular signaling. Cell surface distribution of GM3 is thought to be crucial for the function of GM3, but little is known about the cell surface GM3 distribution. It was shown that anti-GM3 monoclonal antibody binds to GM3 in sparse but not in confluent melanoma cells. Our model membrane study evidenced that monoclonal anti-GM3 antibodies showed stronger binding when GM3 was in less fluid membrane environment. Studies using fluorescent GM3 analogs suggested that GM3 was clustered in less fluid membrane. Moreover, fluorescent lifetime measurement showed that cell surface of high density melanoma cells is more fluid than that of low density cells. Lipidomics and fatty acid supplementation experiment suggested that monounsaturated fatty acid-containing phosphatidylcholine contributed to the cell density-dependent membrane fluidity. Our results indicate that anti-GM3 antibody senses GM3 clustering and the number and/or size of GM3 cluster differ between sparse and confluent melanoma cells.

Tài liệu tham khảo

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