Analysis of Nucleolar Protein Dynamics Reveals the Nuclear Degradation of Ribosomal Proteins

Mayer, 2006, Ribosome biogenesis and cell growth: mTOR coordinates transcription by all three classes of nuclear RNA polymerases, Oncogene, 25, 6384, 10.1038/sj.onc.1209883 Lewis, 2000, Like attracts like: Getting RNA processing together in the nucleus, Science, 288, 1385, 10.1126/science.288.5470.1385 Tate, 2004, The ribosome: Lifting the veil from a fascinating organelle, Bioessays, 26, 582, 10.1002/bies.20022 Granneman, 2004, Ribosome biogenesis: Of knobs and RNA processing, Exp. Cell Res., 296, 43, 10.1016/j.yexcr.2004.03.016 Mann, 2006, Functional and quantitative proteomics using SILAC, Nat. Rev. Mol. Cell Biol., 7, 952, 10.1038/nrm2067 Ross, 2004, Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents, Mol. Cell. Proteomics, 3, 1154, 10.1074/mcp.M400129-MCP200 Andersen, 2005, Nucleolar proteome dynamics, Nature, 433, 77, 10.1038/nature03207 Doherty, 2005, Proteome dynamics in complex organisms: Using stable isotopes to monitor individual protein turnover rates, Proteomics, 5, 522, 10.1002/pmic.200400959 Shav-Tal, 2006, Gene expression within a dynamic nuclear landscape, EMBO J., 25, 3469, 10.1038/sj.emboj.7601226 Jakel, 1998, Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells, EMBO J., 17, 4491, 10.1093/emboj/17.15.4491 Plafker, 2002, Ribosomal protein L12 uses a distinct nuclear import pathway mediated by importin 11, Mol. Cell. Biol., 22, 1266, 10.1128/MCB.22.4.1266-1275.2002 Trinkle-Mulcahy, 2003, Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle, Mol. Biol. Cell, 14, 107, 10.1091/mbc.E02-07-0376 Lippincott-Schwartz, 2003, Photobleaching and photoactivation: Following protein dynamics in living cells, Nat. Cell Biol., 5, S7 Rockel, 2005, Proteasomes degrade proteins in focal subdomains of the human cell nucleus, J. Cell Sci., 118, 5231, 10.1242/jcs.02642 Misteli, 2001, The concept of self-organization in cellular architecture, J. Cell Biol., 155, 181, 10.1083/jcb.200108110 Steitz, 1988, A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells, J. Cell Biol., 106, 545, 10.1083/jcb.106.3.545 Gallagher, 2006, The ARF tumour suppressor, Int. J. Biochem. Cell Biol., 38, 1637, 10.1016/j.biocel.2006.02.008 Wu, 1999, Molecular analysis of the human chromosome 5q13.3 region in patients with hairy cell leukemia and identification of tumor suppressor gene candidates, Genomics, 60, 161, 10.1006/geno.1999.5911 Lebreton, 2006, Nsa2 is an unstable, conserved factor required for the maturation of 27 SB pre-rRNAs, J. Biol. Chem., 281, 27099, 10.1074/jbc.M602199200 Nissan, 2002, 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm, EMBO J., 21, 5539, 10.1093/emboj/cdf547 Schafer, 2003, The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes, EMBO J., 22, 1370, 10.1093/emboj/cdg121 Matsumoto, 2005, Large-scale analysis of the human ubiquitin-related proteome, Proteomics, 5, 4145, 10.1002/pmic.200401280 Stavreva, 2006, Potential roles for ubiquitin and the proteasome during ribosome biogenesis, Mol. Cell. Biol., 26, 5131, 10.1128/MCB.02227-05 Andersen, 2002, Directed proteomic analysis of the human nucleolus, Curr. Biol., 12, 1, 10.1016/S0960-9822(01)00650-9 Scherl, 2002, Functional proteomic analysis of human nucleolus, Mol. Biol. Cell, 13, 4100, 10.1091/mbc.E02-05-0271 Warner, 1979, Distribution of newly formed ribosomal proteins in HeLa cell fractions, J. Cell Biol., 80, 767, 10.1083/jcb.80.3.767 Warner, 1977, In the absence of ribosomal RNA synthesis, the ribosomal proteins of HeLa cells are synthesized normally and degraded rapidly, J. Mol. Biol., 115, 315, 10.1016/0022-2836(77)90157-7 Wu, 1971, Ribosome formation is blocked by camptothecin, a reversible inhibitor of RNA synthesis, Proc. Natl. Acad. Sci. USA, 68, 3009, 10.1073/pnas.68.12.3009 Abovich, 1985, Effect of RP51 gene dosage alterations on ribosome synthesis in Saccharomyces cerevisiae, Mol. Cell. Biol., 5, 3429, 10.1128/MCB.5.12.3429 Warner, 1985, Saccharomyces cerevisiae coordinates accumulation of yeast ribosomal proteins by modulating mRNA splicing, translational initiation, and protein turnover, Mol. Cell. Biol., 5, 1512, 10.1128/MCB.5.6.1512 Gritz, 1985, Posttranslational regulation and assembly into ribosomes of a Saccharomyces cerevisiae ribosomal protein-beta-galactosidase fusion, Mol. Cell. Biol., 5, 3436, 10.1128/MCB.5.12.3436 Leung, 2004, Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells, J. Cell Biol., 166, 787, 10.1083/jcb.200405013 Leung, 2002, In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles, J. Cell Biol., 157, 615, 10.1083/jcb.200201120 Ong, 2006, A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC), Nature Protocols, 1, 2650, 10.1038/nprot.2006.427 Lam, 2006, Isolation of nucleoli, Volume 2, 103