Role of ethylene in germination and seedling growth of littleseed canarygrass (Phalaris minor Retz.)
Tóm tắt
Littleseed canarygrass is a major weed of wheat in rice–wheat cropping system of North-West India. The present study was conducted to study the role of ethylene in germination ecology and seedling growth of littleseed canarygrass and wheat using cobalt chloride (inhibitor of ethylene synthesis) and ethephon (ethylene source). Cobalt chloride at concentrations ≥0.1 mM caused significant reduction in germination (%), germination index and increased the mean germination time of littleseed canarygrass, whereas wheat seeds exhibited significant decrease in germination (%) at cobalt chloride concentrations ≥0.5 mM. Cobalt chloride at higher concentrations significantly reduced the seedling growth of littleseed canarygrass and wheat with more pronounced effect on root length as compared to shoot length. It also significantly increased the membrane leakage and soluble sugar content of seedlings of both species with concomitant decrease in chlorophyll and protein content. Ethephon at 0.1 and 1 mM concentrations significantly increased the germination (%), speed of germination with concomitant decrease in mean germination time as compared to control. Ethephon concentrations 0.1 and 1 mM had no effect on membrane leakage, total soluble sugars and protein content but increased the chlorophyll content in seedlings of both species. Seed dormancy of littleseed canarygrass induced by higher concentrations of cobalt chloride, i.e., 2.5 and 5 mM could also be overcome by treatment with 1 mM ethephon, confirming the role of ethylene in germination of this weed.
Tài liệu tham khảo
Anderson, J. H., & Boardman, N. K. (1964). Studies on greening of dark grown bean plants.VI. Development of photochemical activity. Australian Journal of Biology, 17, 93–101.
Arc, E., Sechet, J., Corbineau, F., Rajjou, L., & Marion, P. A. (2013). ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Frontier Plant Sciences, 4, 1–6.
Association of Official Seed Analysts. (1983). Rules for testing seeds. Journal of Seed Technology, 16, 1–113.
Burhan, N., Shaukat, S. S., & Tahira, A. (2001). Effect of zinc and cobalt on germination and seedling growth of Pennisetum americanum L. and Parkinsonia aculeate L. Pakistan Journal of Biological Sciences, 5, 575–580.
Cochran, W. G., & Cox, G. M. (1966). Experimental designs. New Delhi: Asia Publishing House.
Dhawan, R. S., Dhawan, A. K., Kajla, S., & Moudgil, R. (2005). Assessment of variation in isoproturon in susceptible and resistant biotypes of Phalaris minor Retz. by RAPD analysis. Indian Journal of Biotechnology, 4, 534–537.
Duary, B., & Yaduraju, N. T. (2005). Estimation of yield losses of wheat (Triticum aestivum L.) caused by littleseed canarygrass (Phalaris minor Retz.) competition. Journal of Crop and Weed, 1, 5–8.
Dubois, M., Gilles, K. A., Hamilton, J. K., Roberts, P. A., & Smith, F. (1956). Colorimetric methods for the determination of sugar and related substances. Analytical Chemistry, 28, 350–356.
Ellis, R. A., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science Technology, 9, 373–409.
Fletcher, R. A., & Drexlure, D. M. (1980). Interactions of dichloro-methyl and 2, 4-D in cultivated oats (Avena sativa). Weed Science, 28, 363–366.
Jaleel, C. A., Jayakumar, K., Chang-Xing, Z., & Iqbal, M. (2009). Low concentration of cobalt increases growth, biochemical constituents, mineral status and yield in Zea Mays. Journal of Scientific Research, 1, 128–137.
Jayakumar, K., Jaleel, A., & Vijayarengan, P. (2009). Effect of different concentrations of cobalt on pigment contents of soybean. Botany Research International, 2, 153–156.
Kala, S., & Godara, A. K. (2011). Effect of moisture stress on leaf total proteins, proline and free amino acid content in commercial cultivars of Ziziphus mauritiana. Journal of Scientific Research, 55, 65–69.
Kaur, N., Kaur, T., Kaur, S., & Bhullar, M. S. (2015). Development of cross resistance in Phalaris minor Retz. in Punjab. Agricultural Research Journal, 53, 69–72.
Lalonde, S., & Saini, H. S. (1992). Comparative requirement for endogenous ethylene during seed germination. Annals of Botany, 69, 423–428.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., & Randell, R. J. (1951). Protein measurement with folin-phenol reagent. The Journal of Biological Chemistry, 266, 457–506.
Machabee, S., & Saini, H. S. (1991). Differences in the requirement for endogenous ethylene during germination of dormant and non-dormant seeds of Chenopodium album L. Journal of Plant Physiology, 138, 97–101.
Matilla, A. J. (2000). Ethylene in seed formation and germination. Seed Science Research, 10, 111–126.
Sethi, R., & Kaur, N. (2016). Physiological and biochemical changes associated with abiotic stress tolerance in littleseed canarygrass (Phalaris minor Retz.). Annals of Plant Protection Sciences, 24, 167–173.
Walia, U. S., Brar, L. S., & Singh, B. (2006). Research bulletin-recommendations for weed control in field crops. Ludhiana: Department of Agronomy and Agrometeorology, Punjab Agricultural University.