Single virus detection from the reactive shift of a whispering-gallery mode

Proceedings of the National Academy of Sciences of the United States of America - Tập 105 Số 52 - Trang 20701-20704 - 2008
Frank Vollmer1, Stephen Arnold2, D. Keng2
1The Rowland Institute, Harvard University, Cambridge, MA 02142; and
2MicroParticle PhotoPhysics Lab, Polytechnic Institute of New York University, Brooklyn, NY 11201

Tóm tắt

We report the label-free, real-time optical detection of Influenza A virus particles. Binding of single virions is observed from discrete changes in the resonance frequency/wavelength of a whispering-gallery mode excited in a microspherical cavity. We find that the magnitude of the discrete wavelength-shift signal can be sufficiently enhanced by reducing the microsphere size. A reactive sensing mechanism with inverse dependence on mode volume is confirmed in experiments with virus-sized polystyrene nanoparticles. By comparing the electromagnetic theory for this reactive effect with experiments, the size and mass (≈5.2 × 10 −16 g) of a bound virion are determined directly from the optimal resonance shift.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 105 Số 52

20701-20704

Thông tin tác giả