miR-125b inhibits hepatitis B virus expression in vitro through targeting of the SCNN1A gene

Archives of Virology - Tập 159 Số 12 - Trang 3335-3343 - 2014
Zhang, Zhenzhen1,2,3, Chen, Juan3,4, He, Yin5, Zhan, Xue1, Zhao, Ruiqiu1, Huang, Yanfeng1,2, Xu, Hongmei1, Zhu, Zhaomin1, Liu, Quanbo1
1Department of Infectious Disease, Children’s Hospital of Chongqing Medical University, Chongqing, China
2Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China
3The Key Laboratory of Molecular Biology in Infectious Diseases, Chongqing Medical University, Chongqing, China
4Liver Research Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China
5The Pediatric Department, The Ninth People’s Hospital of Chongqing, Chongqing, China

Tóm tắt

microRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the posttranscriptional level, playing an important role in many diseases. However, reports concerning the role of miRNA in hepatitis B virus (HBV) infection are limited. miRNA chips were used to investigate miRNA changes during HBV infection in vitro. Bioinformatics analysis was used to explore possible miRNA and target genes during HBV infection. The expression of miR-125b and its potential target gene, sodium channel, non-voltage-gated 1 alpha (SCNN1A), was further analyzed. A total of 136 miRNAs were analyzed in an HBV transient transfection model (HepG2-HBV1.3), and 78 miRNAs were differentially expressed in HepG2.2.15 cells compared with HepG2 cells. miR-125b expression was decreased in both HepG2-HBV1.3 and HepG2.2.15 cells, and ectopic expression of miR-125b inhibited HBV DNA intermediates and secretion of HBsAg and HBeAg. miR-125b also inhibited the mRNA and protein levels of SCNN1A. Using a dual luciferase reporter system, we found that SCNN1A was one of the targets of miR-125b. In this study, we found that miR-125b inhibits HBV expression in vitro by regulating SCNN1A expression.

Tài liệu tham khảo

citation_journal_title=Hepatology; citation_title=Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparin sulfate proteoglycans; citation_author=S Andreas, G Philippe, U Stephan; citation_volume=46; citation_publication_date=2007; citation_pages=1759-1768; citation_doi=10.1002/hep.21896; citation_id=CR1 citation_journal_title=Gastroenterology; citation_title=Hepatitis B virus resistance to nucleos(t)ide analogues; citation_author=F Zoulim, S Locarnini; citation_volume=137; citation_issue=5; citation_publication_date=2009; citation_pages=1593-1608; citation_doi=10.1053/j.gastro.2009.08.063; citation_id=CR2 citation_journal_title=J Agric Food Chem; citation_title=Inhibition of the replication of hepatitis B virus in vitro by pu-erh tea extracts; citation_author=S Pei, Y Zhang, H Xu, X Chen, S Chen; citation_volume=59; citation_publication_date=2011; citation_pages=9927-9934; citation_doi=10.1021/jf202376u; citation_id=CR3 citation_journal_title=Virus Res; citation_title=Dioscin’s antiviral effect in vitro; citation_author=C Liu, Y Wang, C Wu, R Pei, J Song, S Chen, X Chen; citation_volume=172; citation_publication_date=2013; citation_pages=9-14; citation_doi=10.1016/j.virusres.2012.12.001; citation_id=CR4 citation_journal_title=Cell; citation_title=MicroRNAs: genomics, biogenesis, mechanism, and function; citation_author=DP Bartel; citation_volume=116; citation_publication_date=2004; citation_pages=281-297; citation_doi=10.1016/S0092-8674(04)00045-5; citation_id=CR5 citation_journal_title=J Biol Chem; citation_title=RISCy business: MicroRNAs, pathogenesis, and viruses; citation_author=B Berkhout, KT Jeang; citation_volume=282; citation_publication_date=2007; citation_pages=26641-26645; citation_doi=10.1074/jbc.R700023200; citation_id=CR6 citation_journal_title=Nature; citation_title=The functions of animal microRNAs; citation_author=V Ambros; citation_volume=431; citation_publication_date=2004; citation_pages=350-355; citation_doi=10.1038/nature02871; citation_id=CR7 citation_journal_title=Hepat Mon; citation_title=Dual role of miR-122 in molecular pathogenesis of viral hepatitis; citation_author=H Sendi; citation_volume=12; citation_issue=5; citation_publication_date=2012; citation_pages=312-314; citation_doi=10.5812/hepatmon.6128; citation_id=CR8 citation_journal_title=J Gastroenterol Hepatol; citation_title=Characterization of microRNA expression profiles associated with hepatitis B virus replication and clearance in vivo and in vitro; citation_author=Xiao-Ben Pan, Hui Ma, Qian Jin, Lai Wei; citation_volume=27; citation_publication_date=2012; citation_pages=805-812; citation_doi=10.1111/j.1440-1746.2011.06979.x; citation_id=CR9 citation_journal_title=Hepatology; citation_title=Loss of microRNA122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G(1) -modulated P53 activity; citation_author=W Jin, Y Wang, L Chen, E Wu, X Ye, GF Gao, F Wang, Y Chen, Z Duan, S Meng; citation_volume=55; citation_issue=3; citation_publication_date=2012; citation_pages=730-741; citation_doi=10.1002/hep.24809; citation_id=CR10 citation_journal_title=Oncol Rep; citation_title=miR-122 inhibits viral replication and cell proliferation in hepatitis B virus-related hepatocellular carcinoma and targets NDRG3; citation_author=CG Fan, CM Wang, C Tian, Y Wang, L Li, WS Sun, RF Li, YG Liu; citation_volume=26; citation_issue=5; citation_publication_date=2011; citation_pages=1281-1286; citation_id=CR11 citation_journal_title=Nucleic Acids Res; citation_title=Human microRNA hsa-miR-125a-5p interferes with expression of hepatitis B virus surface antigen; citation_author=Nicoletta Potenza, Umberto Papa, Nicola Mosca, Francesca Zerbini, Valentina Nobile, Aniello Russo; citation_volume=39; citation_publication_date=2011; citation_pages=5157-5163; citation_doi=10.1093/nar/gkr067; citation_id=CR12 citation_journal_title=PLoS One; citation_title=MicroRNA-141 represses HBV replication by targeting PPARA; citation_author=W Hu, X Wang, X Ding, Y Li, X Zhang, P Xie, J Yang, S Wang; citation_volume=7; citation_issue=3; citation_publication_date=2012; citation_pages=e34165; citation_doi=10.1371/journal.pone.0034165; citation_id=CR13 citation_journal_title=Hepatology; citation_title=Down-regulated MicroRNA-152 induces aberrant DNA methylation in hepatitis B virus-related hepatocellular carcinoma by targeting DNA methyltransferase 1; citation_author=Jinfeng Huang, Yue Wang, Yingjun Guo, Shuhan Sun; citation_volume=52; citation_publication_date=2010; citation_pages=1; citation_id=CR14 citation_journal_title=EMBO J; citation_title=Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation; citation_author=A Weber, J Marquardt, D Elzi, N Forster, S Starke, A Glaum, D Yamada, PA Defossez, J Delrow, RN Eisenman, H Christiansen, M Eilers; citation_volume=27; citation_issue=11; citation_publication_date=2008; citation_pages=1563-1574; citation_doi=10.1038/emboj.2008.85; citation_id=CR15 citation_journal_title=Mol Cancer Ther; citation_title=Induction of the transcriptional repressor ZBTB4 in prostate cancer cells by drug-induced targeting of MicroRNA-17-92/106b-25 clusters; citation_author=K Kim, G Chadalapaka, SS Pathi, UH Jin, JS Lee, YY Park, SG Cho, S Chintharlapalli, S Safe; citation_volume=11; citation_issue=9; citation_publication_date=2012; citation_pages=1852-1862; citation_doi=10.1158/1535-7163.MCT-12-0181; citation_id=CR16 citation_journal_title=Virology; citation_title=Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells; citation_author=Su Chenhe, Zhaohua Hou, Cai Zhang, Zhigang Tian, Jian Zhang; citation_volume=8; citation_publication_date=2011; citation_pages=354; citation_doi=10.1186/1743-422X-8-354; citation_id=CR17 citation_journal_title=Cancer Biol Ther; citation_title=MicroRNA-602 regulating tumor suppressive gene RASSF1A is over-expressed in hepatitis B virus-infected liver and hepatocellular carcinoma; citation_author=Lian Yang, Zhaolong Ma, Dongdong Wang, Wenyue Zhao, Libo Chen, Guobin Wang; citation_volume=9; citation_issue=10; citation_publication_date=2010; citation_pages=803-808; citation_doi=10.4161/cbt.9.10.11440; citation_id=CR18 citation_journal_title=Nat. Med; citation_title=Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+T lymphocytes; citation_author=J Huang, F Wang, E Argyris, K Chen, Z Liang, H Tian, W Huang, K Squires, G Verlinghieri, H Zhang; citation_volume=13; citation_publication_date=2007; citation_pages=1241-1247; citation_doi=10.1038/nm1639; citation_id=CR19 citation_journal_title=Diagn Mol Pathol; citation_title=Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection; citation_author=GJ Nuovo, X Wu, S Volinia, F Yan, G Leva, N Chin, AF Nicol, J Jiang, G Otterson, TD Schmittgen, C Croce; citation_volume=19; citation_publication_date=2010; citation_pages=135-143; citation_doi=10.1097/PDM.0b013e3181c4daaa; citation_id=CR20 citation_journal_title=Int J Mol Sci; citation_title=MicroRNA-125b functions as a tumor suppressor in hepatocellular carcinoma cells; citation_author=HY Jia, YX Wang, WT Yan, HY Li, YZ Tian, SM Wang, HL Zhao; citation_volume=13; citation_issue=7; citation_publication_date=2012; citation_pages=8762-8774; citation_doi=10.3390/ijms13078762; citation_id=CR21 citation_journal_title=Chest; citation_title=Sodium channels and cystic fibrosis; citation_author=SH Donaldson, RC Boucher; citation_volume=132; citation_publication_date=2007; citation_pages=1631-1636; citation_doi=10.1378/chest.07-0288; citation_id=CR22 citation_journal_title=Physiol Rev; citation_title=Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure; citation_author=S Kellenberger, L Schild; citation_volume=82; citation_publication_date=2002; citation_pages=735-767; citation_id=CR23 citation_journal_title=Am J Physiol Endocrinol, Meta; citation_title=A homozygous missense mutation in SCNN1A is responsible for a transient neonatal form of pseudohypoaldosteronism type 1; citation_author=M Dirlewanger, D Huser, MC Zennaro, E Girardin, L Schild, VM Schwitzgebel; citation_volume=301; citation_issue=3; citation_publication_date=2011; citation_pages=467-473; citation_doi=10.1152/ajpendo.00066.2011; citation_id=CR24