Chemotactic factor-induced effects upon deformability of human polymorphonuclear leukocytes
Tóm tắt
We have compared the effects of the synthetic chemotactic peptide,N-formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe), on the deformability and chemotaxis of human polymorphonuclear leukocytes (PMNs). By utilizing the technique of cell elastimetry (deformability), which measures the negative pressure required to aspirate cells into a micropipet, we have shown a correlation between f-Met-Leu-Phe concentrations and PMN deformability which parallels the effects of that chemoattractant on filter chemotaxis. At concentrations as low as 10−11
M, a change in the deformability characteristics of a population of neutrophils was demonstrated. The increase in deformability became most marked at peptide concentrations in the range of 1–5×10−9
M, corresponding to the optimal concentration range in the filter chemotaxis assay. Concentrations of 10−8
M and greater were associated with a significant increase in the negative pressure required to aspirate the cells into a micropipet. Alterations in PMN deformability, therefore, occur as a natural consequence of stimulation of a human PMN with a chemotactic factor and may be a prerequisite to efficient movement.
Tài liệu tham khảo
Gallin JI, Quie PG (eds): Leukocyte Chemotaxis: Methods, Physiology and Clinical Implications. New York, Raven Press, 1978
Lichtman MA: Cellular deformability during maturation of the myeloblast. Possible role in marrow egress. N Engl J Med 283:943–948, 1970
Miller ME, Myers KA: Membrane deformability of human polymorphonuclear leukocytes and its relationship to development of normal and abnormal PMN movement.In Development of Host Defenses, DA Dayton, MD Cooper (eds). New York, Raven Press, 1977, pp 175–185
Miller ME: Cell elastimetry in the study of normal and abnormal movement of human neutrophils. Clin Immunol Immunopathol 14:503–510, 1979
Bøyum A: Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21 (Suppl 97):77–89, 1968
Showell HJ, Freer RJ, Zigmond SH, Schiffmann E, Aswanikumar S, Corcoran B, Becker EL: The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal enzyme secretion for neutrophils. J Exp Med 143:1154–1169, 1976
Fehr J, Dahinden C: Modulating influence of chemotactic factor-induced cell adhesiveness on granulocyte function. J Clin Invest 64:8–16, 1979