Distribution of IGF-I mRNA and IGF-I binding sites in the rat kidney

Springer Science and Business Media LLC - 1992
G. L. Matejka1, P. S. Eriksson1, B. Carlsson2, E. Jennische1
1Department of Histology, University of Göteborg, Göteborg, Sweden
2Department of Physiology, University of Göteborg, Göteborg, Sweden

Tóm tắt

In the present study we have investigated the distribution of IGF-I mRNA and IGF-I binding sites in the rat kidney. The distribution of IGF-I mRNA was investigated using a simple and sensitive non-radioactive in situ hybridisation technique based on probe labelling with digoxigenin labelled-UTP followed by detection with conventional immunocytochemical techniques. IGF-I mRNA was found predominantly in medullary collecting ducts and sparsely in cortical collecting duct cells. In addition IGF-I mRNA was expressed in scattered proximal tubular cells in the cortex and in cells confined to the glomerular tuft. IGF-I binding sites were studied using radiolabelled IGF-I and conventional autoradiographical techniques on tissue sections. It was found that IGF-I binding sites were widely distributed throughout the entire kidney and that the specific binding was highest in the inner medulla. These findings add further complexity to the understanding of IGF-I production and action on renal structures.

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