Melatonin reduces apoptotic cells, SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts

Reproduction in Domestic Animals - Tập 53 Số 1 - Trang 226-236 - 2018
Thyago Carvalho Marques1, EC da Silva Santos1, Tiago Omar Diesel1, L. O. Leme2, Charles Ferreira Martins3, M. A. N. Dode2, Bênner Geraldo Alves4, Fph Costa1, EB de Oliveira1, Maria Lúcia Gambarini1
1Center for Studies and Research in Animal Reproductive Biology College of Veterinary and Animal Science Federal University of Goiás Goiânia GO Brazil
2Embrapa Genetic Resources and Biotechnology Laboratory of Animal Reproduction Brasília DF Brazil
3Center of Animal Production Systems Embrapa Cerrados Brasília DF Brazil
4Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil

Tóm tắt

ContentsEffects of adding different concentrations of melatonin (10−7, 10−9 and 10−11 M) to maturation (Experiment 1; Control, IVM + 10−7, IVM + 10−9, IVM + 10−11) and culture media (Experiment 2; Control, IVC + 10−7, IVC + 10−9, IVC + 10−11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10−9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10−9, IVC + 10−9, IVM/IVC + 10−9). In Experiment 1, maturated oocytes from Control and IVM + 10−9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10−7 (43.5%; 56.7%) and IVC + 10−9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10−9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10−9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM/IVC + 10−9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10−9 treatment also had a higher mRNA expression of antioxidant gene (SOD2) compared to the Control, as well as the heat shock protein (HSPB1) compared to the IVM + 10−9. Reactive oxygen species production was greater in the IVM/IVC + 10−9 treatment group. In conclusion, the 10−9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.

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Reproduction in Domestic Animals

Tập 53 Số 1

226-236

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