Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths

Tsien, 1984, Measuring and manipulating cytosolic Ca2+ with trapped indicators, Trends Biochem. Sci., 9, 263, 10.1016/0968-0004(84)90160-9 Rink, 1985, Cell Calcium Tsien, 1982, Calcium homeostasis in intact lymphocytes: cytoplasmic free Ca2+ monitored with a new, intracellularly trapped fluorescent indicator, J. Cell Biology, 94, 325, 10.1083/jcb.94.2.325 Tsien, 1981, A non-disruptive technique for loading calcium buffers and indicators into cells, Nature, 290, 527, 10.1038/290527a0 Kruskal, 1984, Thyrotropin-releasing hormone-induced changes in intracellular [Ca2+] measured by microspectrofluorometry of individual quin2-loaded cells, J. Cell Biology, 99, 1167, 10.1083/jcb.99.3.1167 Williams, 1985, Ca2+ measured in a single smooth muscle cell using a new powerfully fluorescent dye (fura-2) and the digital image microscope, Biophys. Soc. Abstracts Grynkiewicz, 1985, A new generation of Ca2+ indicators with greatly improved fluorescence properties, J. Biol. Chem., 10.1016/S0021-9258(19)83641-4 Paradiso, 1985, Na+H+ and Cl−HCO3− exchange in oxyntic cells of rabbit gastric glands Rink, 1982, Cytoplasmic pH and free Mg2+ in lymphocytes, J. Cell Biology, 95, 189, 10.1083/jcb.95.1.189 Minta, A. and Tsien, R.Y. Manuscript in preparation. Heiple, 1982, An optical technique for measurement of intracellular pH in single living cells, 22 Poenie, M. and Tsien, R.Y. Manuscript in preparation. Hallam, 1984, Stimulus-response coupling in platelets, Biochem. J., 218, 819, 10.1042/bj2180819 Unpublished results of H. Alexander Wilson and Thomas M. Chused, Laboratory of Microbial Immunity, NIAID, National Institutes of Health, in collaboration with R.Y.T. Tanasugarn, 1984, Microspectrofluorometry by digital image processing: measurement of cytoplasmic pH, J. Cell Biology, 98, 717, 10.1083/jcb.98.2.717