An alternative method to prepare samples of the pathogenic yeast Cryptococcus neoformans for scanning electron microscopy analysis

Casadevall, 1998, Biochemistry, 71 Casali, 2003, Molecular typing of clinical and environmental Cryptococcus neoformans isolates in the Brazilian state Rio Grande do Sul, FEMS Yeast Res., 3, 405, 10.1016/S1567-1356(03)00038-2 Cleare, 1999, Scanning electron microscopy of encapsulated and non-encapsulated Cryptococcus neoformans and the effect of glucose on capsular polysaccharide release, Med. Mycol., 37, 235 Franzot, 1999, Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates, J. Clin. Microbiol., 37, 838, 10.1128/JCM.37.3.838-840.1999 Janbon, 2004, Cryptococcus neoformans capsule biosynthesis and regulation, FEMS Yeast Res., 4, 765, 10.1016/j.femsyr.2004.04.003 Horta, 2002, Epidemiological aspects of clinical and environmental Cryptococcus neoformans isolates in the Brazilian state Rio Grande do Sul, Med. Mycol., 40, 1, 10.1080/714031150 Katsu, 2004, The internal transcribed spacers and 5.8 S rRNA gene show extensive diversity among isolates of the Cryptococcus neoformans species complex, FEMS Yeast Res., 4, 377, 10.1016/S1567-1356(03)00176-4 Nosanchuk, 1999, Amphotericin B and Fluconazole affect cellular charge, macrophage phagocytosis, and cellular morphology of Cryptococcus neoformans at sub inhibitory concentrations, Antimicrob. Agents Chemother., 43, 233, 10.1128/AAC.43.2.233 Sorrell, 2001, Cryptococcus neoformans variety gattii, Med. Mycol., 39, 155, 10.1080/mmy.39.2.155.168