Cholesterol stimulation of penetration of unilamellar liposomes by hydrophobic compounds
Tóm tắt
The incorporation of cholesterol into unilamellar liposomes greatly increased the transmembranous movement of hydrophobic ionophores such as nigericin. In reconstituted liposomes containing rhodopsin as the only protein, the presence of cholesterol lowers by 10-fold or more the amount of nigericin required to eliminate the light-driven proton gradient. These effects are seen both above and below the transition temperature of the phospholipid used for reconstitution. Cholesterol similarly increases the ability of A-23187, 1799, or NH4SCN to collapse the proton gradient of bacteriorhodopsin vesicles. Cholesterol also lowers the concentration of nigericin or valinomycin required for a rapid translocation of Rb+ into protein-free liposomes. It also lowers the concentration of A-23187 required for the release of Ca45 trapped in protein-free liposomes. In contrast to these observations and in confirmation of previous findings, we observed that cholesterol decreased the permeability of liposomes for glucose. Thus the effects of cholesterol on the permeability of the membrane vary with the chemical nature of the permeating compounds. We have also confirmed that in multilamellar liposomes cholesterol decreases the permeability of Rb+ in the presence of valinomycin. It therefore appears that the effect of cholesterol changes with the size and structural features of the model membranes.
Tài liệu tham khảo
Bakker, E.P., Van DenHeuvel, E.J., Weichmann, A.H.C.A., Van Dam, K. 1973. A comparison between the effectiveness of uncouplers of oxidative phosphorylation in mitochondrial and in different artificial membrane systems.Biochim. Biophys. Acta 292:78
Bruckdorfer, K.R., Demel, R.A., DeGier, J., Van Deenen, L.L.M. 1969. The effect of partial replacements of membrane cholesterol by other steroids on the osmotic fragility and glycerol permeability of erythrocytes.Biochim. Biophys. Acta 183:334
Darke, A., Finer, E.G., Flook, A.G., Phillips, M.C. 1972. Nuclear magnetic resonance study of lecithin-cholesterol interactions.J. Mol. Biol. 63:265
DeGier, J., Haest, C.W.M., Mandersloot, J.G., Van Deenen, L.L.M. 1970. Valinomycin-induced permeation of86Rb+ of liposomes with varying composition through the bilayers.Biochim. Biophys. Acta 211:373
DeGier, J., Mandersloot, J.G., Van Deenen, L.L.M. 1968. Lipid composition and permeability of liposomes.Biochim. Biophys. Acta 150:666
DeGier, J., Mandersloot, J.G., Van Deenen, L.L.M. 1969. The role of cholesterol in lipid membranes.Biochim. Biophys. Acta 173:143
DeKruyff, B., DeGreef, W.J., Van Eyk, R.V.W., Demel, R.A., Van Deenen, L.L.M. 1973. The effect of different fatty acid and sterol composition on the erythritol flux through the cell membranes ofAcholeplasma laidlawii.Biochim. Biophys. Acta 298:479
DeKruyff, B., Demel, R.A., Van Deenen, L.L.M. 1972. The effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intactAcholeplasma laidlawii cell membranes and derived liposomes.Biochim. Biophys. Acta 255:331
Demel, R.A., Bruckdorfer, K.R., Van Deenen, L.L.M. 1972. The effect of sterol structure on the permeability of liposomes to glucose, glycerol, and Rb+.Biochim. Biophys. Acta 255:321
Demel, R.A., Geurts Van Kessel, W.S.M., Van Deenen, L.L.M. 1972. The properties of polyunsaturated lecithins in monolayers and liposomes and the interactions of these lecithins with cholesterol.Biochim. Biophys. Acta 266:26
Demel, R.A., Kinsky, S.C., Kinsky, C.B., Van Deenen, L.L.M. 1968. Effects of temperature and cholesterol on the glucose permeability of liposomes prepared with natural and synthetic lecithins.Biochim. Biophys. Acta 150:655
Gasko, O.D., Knowles, A.F., Shertzer, H.G., Suolinna, E.-M., Racker, E. 1976. The use of ion-exchange resins for studying ion transport in biological systems.Anal. Biochem. 72:57
Gottfried, E.L., Rapport, M.M. 1962. The biochemistry of plasmalogens.J. Biol. Chem. 237:329
Hinkle, P., Kasahara, M. 1976. Reconstitution ofd-glucose transport catalyzed by a protein fraction from human erythrocytes in sonicated liposomes.Proc. Nat. Acad. Sci. USA 73:396
Hubbell, W.L., McConnell, H.M. 1968. Spin-label studies of the excitable membranes of nerve and muscle.Proc. Nat. Acad. Sci. USA 61:12
Hubbell, W.L., McConnell, H.M. 1969. Orientation and motion of amphiphilic spin labels in membranes.Proc. Nat. Acad. Sci. USA 64:20
Inesi, G., Millman, M., Eletr, S. 1973. Temperature-induced transitions of function and structure in sarcoplasmic reticulum membranes.J. Mol. Biol. 81:483
Inoue, K. 1974. Permeability properties of liposomes prepared from dipalmitoyl lecithin, dimyristoyl lecithin, egg lecithin, rat liver lecithin, and beef brain sphingomyelin.Biochim. Biophys. Acta 339:390
Kagawa, Y., Racker, E. 1971. Partial resolution of the enzymes catalyzing oxidative phosphorylation XXV. Reconstitution of vesicles catalyzing32Pi-adenosine triphosphate exchange.J. Biol. Chem. 246:5477
Kanner, B.I., Racker, E. 1975. Light-dependent proton and rubidium translocation in membrane vesicles fromHalobacterium halobium.Biochem. Biophys. Res. Commun. 64:1054
Kimelberg, H.K., Papahadjopoulos, D. 1974. Effects of phospholipid acyl chain fluidity, phase transitions, and cholesterol on (Na++K+)-stimulated adenosine triphosphatase.J. Biol. Chem. 249:1071
Kroes, J., Ostwald, R. 1971. Erythrocyte membranes—effect of increased cholesterol content on permeability.Biochim. Biophys. Acta 249:647
Ladbrooke, B.D., Chapman, D. 1969. Thermal analysis of lipids, proteins, and biological membranes.Chem. Phys. Lip. 3:304
Ladbrooke, B.D., Williams, R.M., Chapman, D. 1968. Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and Xray-diffraction.Biochim. Biophys. Acta 150:333
Lelievre, J., Rich, G.T. 1973. The permeability of lipid membranes to non-electrolytes.Biochim. Biophys. Acta 298:15
Linden, C.D., Wright, K.L., McConnell, H.M., Fox, C.F. 1973. Lateral phase separations in membrane lipids and the mechanism of sugar transport inEscherichia coli.Proc. Nat. Acad. Sci. USA 70:2271
McElhaney, R.N., DeGier, J., VanderNeut-Kok, E.C.M. 1973. The effect of alterations in fatty acid composition and cholesterol content on the non-electrolyte permeability ofAcholeplasma laidlawii B cells and derived liposomes.Biochim. Biophys. Acta 298:500
Newman, G.C., Huang, C. 1975. Structural studies on phosphatidylcholine-cholesterol mixed vesicles.Biochemistry 14:3363
Oldfield, E., Chapman, D. 1971. Effect of cholesterol and cholesterol derivatives on hydrocarbon chain mobility in lipids.Biochem. Biophys. Res. Commun. 43:610
Oldfield, E., Chapman, D. 1972. Molecular dynamics of cerebroside-cholesterol and sphingomyelin-cholesterol interactions.FEBS Letters 21:303
Oldfield, E., Chapman, D. 1972. Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol.FEBS Letters 23:285
Overath, P., Trauble, H. 1973. Phase transitions in cells, membranes, and lipids ofEscherichia coli. Detection by fluorescent probes, light scattering and dilatometry.Biochemistry 12:2625
Papahadjopoulos, D., Jacobson, K., Nir, S., Isac, T. 1973. Phase transitions in phospholipid vesicles: Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol.Biochim. Biophys. Acta 311:330
Papahadjopoulos, D., Miller, N. 1967. Phospholipid model membranes: Structural characteristics of hydrated liquid crystals.Biochim. Biophys. Acta 135:624
Pfeiffer, D.R., Lardy, H.A. 1976. Ionophore A-23187: The effect of H+ concentration on complex formation with divalent and monovalent cations and the demonstration of K+ transport in mitochondria mediated by A-23187.Biochemistry 15:935
Racker, E. 1973. A new procedure for the reconstitution of biologically active phospholipid vesicles.Biochem. Biophys. Res. Commun. 55:224
Racker, E., Hinkle, P.C. 1974. Effect of temperature on the function of a proton pump.J. Membrane Biol. 17:181
Racker, E., Knowles, A.F., Eytan, E. 1975. Resolution and reconstitution of iontransport systems.Ann. N.Y. Acad. Sci. 264:17
Racker, E., Stoeckenius, W. 1974. Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation.J. Biol. Chem. 249:662
Rottem, S., Cirillo, V.P., DeKruyff, B., Shinitzky, M., Razin, S. 1973. Cholesterol in mycoplasma membranes.Biochim. Biophys. Acta 323:509
Schmid, H.H.O., Takahashi, T. 1968. The Alk-1-enyl ether and alkyl ether lipids of bovine heart muscle.Biochim. Biophys. Acta 164:141
Szabo, G., Eisenman, G., Ciani, S. 1969. The effects of the macrotetralide actin antibotics on the electrical properties of phospholipid bilayer membranes.J. Membrane Biol. 1:346
Tsong, T.Y. 1975. Transport of 8-anilino-1-naphthalene sulfonate as a probe of the effect of cholesterol on the phospholipid bilayer structures.Biochemistry 14:5415
Yeagle, P.L., Hutton, W.C., Huang, C., Martin, R.B. 1975. Headgroup conformation and lipid-cholesterol association in phosphatidylcholine vesicles: A31P {1H} nuclear Overhauser effect study.Proc. Nat. Acad. Sci. USA 72:3477