Functionalization of Ag Nanoparticles by Glutamic Acid and Conjugation of Ag@Glu by Thiosemicarbazide Enhances the Apoptosis of Human Breast Cancer MCF-7 Cells
Tóm tắt
In this study, we developed a novel thiosemicarbazide (TSC) conjugated with Ag nanoparticles functionalized by glutamic acid (Ag@Glu) for anticancer activities against human breast cancer MCF-7 cells. The Ag@Glu/TSC nanoparticles were characterized by UV–Vis diffuse reflectance spectroscopy, Fourier transform infrared (FTIR) spectroscopy, SEM, TEM, and XRD analyses. FTIR spectrum showed that the TSC was anchored on the Ag@Glu nanoparticles. The TEM and SEM images of the sample revealed that the Ag@Glu/TSC varied in morphology with a mean size of ~ 50 nm. In vitro cytotoxicity effect of Ag@Glu/TSC was performed using MTT assay toward human breast cancer MCF-7 cells. Moreover, Ag@Glu/TSC induced-apoptosis was evaluated using Hoechst 33258 staining, Caspase-3 activation assay, and annexin V/PI staining with flow cytometry analysis. The MTT assay result of Ag@Glu/TSC showed that the cell viability was in a dose-dependent manner (IC50 = 299.17 μg/mL). We found that Ag@Glu/TSC induce the apoptosis of MCF-7 cell through an increase in Caspase-3 and nuclear fragmentation. Furthermore, the percentage of early and late apoptotic cancer cells was increased as compared to untreated cells using annexin V/PI staining. Finally, we found that the novel Ag@Glu/TSC nanoparticles could inhibit the proliferation of MCF-7 cells by triggering apoptosis pathway, suggesting a new approach to treat breast cancer.
Tài liệu tham khảo
J. Ferlay, I. Soerjomataram, R. Dikshit, S. Eser, C. Mathers, M. Rebelo, D. M. Parkin, D. Forman, and F. Bray (2015). Int. J. Cancer 136, 359.
J. Lee, D. K. Chatterjee, M. H. Lee, and S. Krishnan (2014). Cancer Lett. 347, 46.
M. Fakruddin, Z. Hossain, and H. Afroz (2012). J. Nanobiotechnol. 10, 31.
R. G. Saratale, H. S. Shin, G. Kumar, G. Benelli, D. S. Kim, and G. D. Saratale (2015). Artif. Cells Nanomed. Biotechnol. 46, 211.
K. Murugan, D. Dinesh, K. Kavithaa, M. Paulpandi, T. Ponraj, M. S. Alsalhi, S. Devanesan, J. Subramaniam, R. Rajaganesh, H. Wei, S. Kumar, M. Nicoletti, and G. Benelli (2016). Parasitol. Res. 115, (3), 1085.
M. Z. Ahmad, S. Akhter, G. K. Jain, M. Rahman, S. A. Pathan, F. J. Ahmad, and R. K. Khar (2010). Expert. Opin. Drug Deliv. 7, 42.
G. Benelli and C. M. Lukehart (2017). J. Clust. Sci. 28, 1–2. https://doi.org/10.1007/s10876-017-1165-5.
G. Benelli, F. Maggi, R. Petrelli, and M. Nicoletti (2017). J. Clust. Sci. 28, (1), 3–10.
P. Venkatachalam, T. Kayalvizhi, J. Udayabanu, G. Benelli, and N. Geetha (2017). J. Clust. Sci. 28, (1), 607.
X. Zhao, Y. Xia, Q. Li, X. Ma, F. Quan, C. Geng, and Z. Han (2014). Colloids Surf. A Physicochem. Eng. Asp. 444, 180.
R. K. Madhu, R. B. Lakshmeesha, S. Asha, B. Narayana, K. Byrappa, W. Youjiang, Y. Donggang, and Y. Sangappa (2015). Adv. Mater. Lett. 6, 1088.
A. S. Kutsenko and V. M. Granchak (2009). Theor. Exp. Chem. 45, 313.
G. Benelli (2016). Enzyme. Microb. Technol. 95, 58–68.
V. Asgary, A. Shoari, F. Baghbani, S. A. Sadat Shandiz, M. S. Khosravi, A. Janani, R. Bigdeli, R. Bashar, and R. A. Cohan (2016). Int. J. Nanomed. 11, 3597.
E. M. Bavin, R. J. W. Rees, J. M. Robson, M. Seiler, D. E. Seymour, and D. Suddaby (1950). J. Pharm. Pharmacol. 2, 72.
R. A. Finch, M. C. Liu, A. H. Cory, J. G. Cory, and A. C. Sartorelli (1999). Adv. Enzyme Regul. 39, 3.
H. Beraldo and D. Gambino (2004). Mini-Rev. Med. Chem. 4, 9.
J. L. Navarrete, V. Hernandez, and F. J. Ramirez (1995). J. Mol. Struct. 348, 249.
L. Jin and Y. Wang (2017). Phys. Chem. Chem. Phys. 19, 12992.
K. C. Barick, A. Sharma, N. G. Shetake, R. S. Ningthoujam, R. K. Vatsa, P. D. Babu, B. N. Pandey, and P. A. Hassan (2015). Dalton Trans. 44, 14686.
B. He, N. Lu, and Z. Zhou (2009). Curr. Opin. Cell Biol. 21, 900.
R. A. V. Bell and L. A. Megeney (2017). Cell Death Differ. 24, 1359.
V. S. Eckle, A. Buchmann, W. Bursch, R. Schulte-Hermann, and M. Schwarz (2004). Toxicol. Pathol. 32, 9.
P. Krishnan, M. Rajan, S. Kumari, S. Sakinash, S. P. Priya, F. Amira, L. Danjuma, et al. (2017). Sci. Rep. 7, (1), 10962.