Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes

Baxter, 2010, Generating hepatic cell lineages from pluripotent stem cells for drug toxicity screening, Stem Cell Res, 5, 4, 10.1016/j.scr.2010.02.002 Zhang, 2013, Generation, characterization and potential therapeutic applications of mature and functional hepatocytes from stem cells, J Cell Physiol, 228, 298, 10.1002/jcp.24150 Agarwal, 2008, Efficient differentiation of functional hepatocytes from human embryonic stem cells, Stem Cells, 26, 1117, 10.1634/stemcells.2007-1102 Basma, 2009, Differentiation and transplantation of human embryonic stem cell-derived hepatocytes, Gastroenterology, 136, 990, 10.1053/j.gastro.2008.10.047 Brolen, 2010, Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage, J Biotechnol, 145, 284, 10.1016/j.jbiotec.2009.11.007 Hannan, 2013, Production of hepatocyte-like cells from human pluripotent stem cells, Nat Protoc, 8, 430, 10.1038/nprot.2012.153 Hay, 2008, Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling, Proc Natl Acad Sci U S A, 105, 12301, 10.1073/pnas.0806522105 Medine CN, Lucendo-Villarin B, Zhou W, West CC, Hay DC. Robust generation of hepatocyte-like cells from human embryonic stem cell populations. StemBook (Harvard Stem Cell Institute) 2012:http://www.stembook.org/node/736. Roelandt, 2010, Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells, PLoS One, 5, e12101, 10.1371/journal.pone.0012101 Si-Tayeb, 2010, Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells, Hepatology, 51, 297, 10.1002/hep.23354 Song, 2009, Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells, Cell Res, 19, 1233, 10.1038/cr.2009.107 Touboul, 2010, Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development, Hepatology, 51, 1754, 10.1002/hep.23506 Cai J, DeLaForest A, Fisher J, Urick A, Wagner T, Twaroski K, et al. Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate. StemBook (Harvard Stem Cell Institute) 2012:http://www.stembook.org/node/721. Chen, 2012, Rapid generation of mature hepatocyte-like cells from human induced pluripotent stem cells by an efficient three-step protocol, Hepatology, 55, 1193, 10.1002/hep.24790 D’Amour, 2006, Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells, Nat Biotech, 24, 1392, 10.1038/nbt1259 Hay, 2008, Efficient differentiation of hepatocytes from human embryonic stem cells exhibiting markers recapitulating liver development in vivo, Stem Cells, 26, 894, 10.1634/stemcells.2007-0718 Hewitt, 2007, Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies, Drug Metab Rev, 39, 159, 10.1080/03602530601093489 Hay, 2011, Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism, Stem Cell Res, 6, 92, 10.1016/j.scr.2010.12.002 Rowe, 2013, Proteome-wide analyses of human hepatocytes during differentiation and dedifferentiation, Hepatology, 58, 799, 10.1002/hep.26414 Jozefczuk, 2011, Comparative analysis of human embryonic stem cell and induced pluripotent stem cell-derived hepatocyte-like cells reveals current drawbacks and possible strategies for improved differentiation, Stem Cells Dev, 20, 1259, 10.1089/scd.2010.0361 Baxter, 2009, Analysis of the distinct functions of growth factors and tissue culture substrates necessary for the long-term self-renewal of human embryonic stem cell lines, Stem Cell Res, 10.1016/j.scr.2009.03.002 Rashid, 2010, Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells, J Clin Invest, 120, 3127, 10.1172/JCI43122 Jennings, 2013, Development of the human pancreas from foregut to endocrine commitment, Diabetes, 62, 3514, 10.2337/db12-1479 Hwang-Verslues, 2010, HNF4alpha–role in drug metabolism and potential drug target?, Curr Opin Pharmacol, 10, 698, 10.1016/j.coph.2010.08.010 DeLaForest, 2011, HNF4A is essential for specification of hepatic progenitors from human pluripotent stem cells, Development, 138, 4143, 10.1242/dev.062547 Parviz, 2003, Hepatocyte nuclear factor 4alpha controls the development of a hepatic epithelium and liver morphogenesis, Nat Genet, 34, 292, 10.1038/ng1175 Nyirenda, 2006, Prenatal programming of hepatocyte nuclear factor 4alpha in the rat: a key mechanism in the ‘fetal origins of hyperglycaemia’?, Diabetologia, 49, 1412, 10.1007/s00125-006-0188-5 Harries, 2008, The diabetic phenotype in HNF4A mutation carriers is moderated by the expression of HNF4A isoforms from the P1 promoter during fetal development, Diabetes, 57, 1745, 10.2337/db07-1742 Stanulovic, 2007, Hepatic HNF4alpha deficiency induces periportal expression of glutamine synthetase and other pericentral enzymes, Hepatology, 45, 433, 10.1002/hep.21456 Omary, 2009, Toward unraveling the complexity of simple epithelial keratins in human disease, J Clin Invest, 119, 1794, 10.1172/JCI37762 Matsukuma, 2012, Aberrant cytokeratin 7 expression of centrilobular hepatocytes: a clinicopathological study, Histopathology, 61, 857, 10.1111/j.1365-2559.2012.04278.x Sancho-Bru, 2012, Liver progenitor cell markers correlate with liver damage and predict short-term mortality in patients with alcoholic hepatitis, Hepatology, 55, 1931, 10.1002/hep.25614 Ge, 2005, Interpreting expression profiles of cancers by genome-wide survey of breadth of expression in normal tissues, Genomics, 86, 127, 10.1016/j.ygeno.2005.04.008 Zhao, 2013, Promotion of the efficient metabolic maturation of human pluripotent stem cell-derived hepatocytes by correcting specification defects, Cell Res, 23, 157, 10.1038/cr.2012.144 Woo, 2012, Direct and indirect contribution of human embryonic stem cell-derived hepatocyte-like cells to liver repair in mice, Gastroenterology, 142, 602, 10.1053/j.gastro.2011.11.030 Zhang, 2011, Rescue of ATP7B function in hepatocyte-like cells from Wilson’s disease induced pluripotent stem cells using gene therapy or the chaperone drug curcumin, Hum Mol Genet, 20, 3176, 10.1093/hmg/ddr223 Sullivan, 2010, Generation of functional human hepatic endoderm from human induced pluripotent stem cells, Hepatology, 51, 329, 10.1002/hep.23335 Shan, 2013, Identification of small molecules for human hepatocyte expansion and iPS differentiation, Nat Chem Biol, 9, 514, 10.1038/nchembio.1270