Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals’ pollution in coastal areas from the Saronikos Gulf of Greece

Adami, 2002, Levels of cadmium and zinc in hepatopancreas of reared Mytilus galloprovincialis from the Gulf of Trieste (Italy), Chemosphere, 48, 671, 10.1016/S0045-6535(02)00196-0 Aebi, 1984, Catalase in vitro, Methods Enzymol., 105, 121, 10.1016/S0076-6879(84)05016-3 Arabi, 2005, Metal-ion-mediated oxidative stress in the gill homogenate of rainbow trout (Oncorhynchus mykiss): antioxidant potential of manganese, selenium, and albumin, Biol. Trace Elem. Res., 108, 155, 10.1385/BTER:108:1-3:155 Bebianno, 2005, Antioxidant systems and lipid peroxidation in Bathymodiolusazoricus from Mid-Atlantic Ridge hydrothermal vent fields, Aquat. Toxicol., 75, 354, 10.1016/j.aquatox.2005.08.013 Borković, 2005, The activity of antioxidant defence enzymes in the mussel Mytilus galloprovincialis from the Adriatic Sea, Comp. Biochem. Physiol. C Toxicol. Pharmacol., 141, 366, 10.1016/j.cbpc.2005.08.001 Buege, 1978, Microsomal lipid peroxidation, Methods Enzymol., 52, 302, 10.1016/S0076-6879(78)52032-6 Canesi, 1998, In vitro and in vivo effects of heavy metals on mussel digestive gland hexokinase activity: the role of glutathione, Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol., 120, 261, 10.1016/S0742-8413(98)10004-X Catsiki, 2006, Study on the behavior of the heavy metals Cu, Cr, Ni, Zn, Fe, Mn and 137Cs in an estuarine ecosystem using Mytilus galloprovincialis as a bioindicator species: the case of Thermaikos gulf, Greece, J. Environ. Radiact., 86, 31, 10.1016/j.jenvrad.2005.07.005 1996 Cohen, 1996, A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures, J. Neurosci. Methods, 67, 53, 10.1016/0165-0270(96)00011-8 Cotou, 2001, Metallothionein levels in Callista chione and Venus verrucosa from two areas of Eastern Mediterranean (Greece), Z. Naturforsch., 56c, 848, 10.1515/znc-2001-9-1028 Di Giulio, 1993, Effects of black rock harbour sediments on indices of biotransformation, oxidative stress and DNA integrity in channel fish, Aquat. Toxicol., 26, 1, 10.1016/0166-445X(93)90002-I Filho, 2001, Seasonal changes in antioxidant defences of the digestive gland of the brown mussel (Perna perna), Aquaculture, 203, 149, 10.1016/S0044-8486(01)00599-3 Firfilionis, 2004, The removal of trace metals at the wastewater treatment pant of Psyttalia, Mediterr. Mar. Sci., 5, 71, 10.12681/mms.212 Flöhe, 1984, Superoxide dismutase assays, Methods Enzymol., 105, 93, 10.1016/S0076-6879(84)05013-8 Frenzilli, 2004, Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment, Mar. Environ. Res., 58, 609, 10.1016/j.marenvres.2004.03.050 Goldberg, 2000, Beyond the mussel watch – new directions for monitoring marine pollution, Sci. Total Environ., 247, 165, 10.1016/S0048-9697(99)00488-X Greenwald, 1985 HCMR, 2003. Monitoring of the effects of pollution along the Saronikos Gulf. Final Report, Athens. Kalpaxis, 2004, Biomonitoring of gulf of Patras, N. Peloponnesus, Greece. Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells, Environ. Res., 94, 211, 10.1016/S0013-9351(03)00048-3 Lau, 2003, Effect of size, tissue parts and location on six biochemical markers in the green-lipped mussel, Perna viridis, Mar. Pollut. Bull., 46, 1563, 10.1016/S0025-326X(03)00321-7 Livingstone, 1998, Organic xenobiotics in aquatic ecosystems: quantitative and qualitative differences in biotrasformation by invertebrates and fish, Comparat. Environ. Physiol., 120A, 43 Livingstone, 2001, Contaminated-stimulated reactive oxygen species production and oxidative damage in aquatic organisms, Mar. Pollut. Bull., 42, 656, 10.1016/S0025-326X(01)00060-1 Lionetto, 2001, Biomonitoring of heavy metal contamination along the Salerno coast (Italy) by metallothionein evaluation in Mytilus galloprovincialis and Mullus barbatus, Aquat. Conserv. Mar. Freshw. Ecosyst., 11, 305, 10.1002/aqc.458 Manduzio, 2003, Characterization of an inducible isoform of the Cu/Zn superoxide dismutase in the blue mussel Mytilus edulis, Aquat. Toxicol., 64, 73, 10.1016/S0166-445X(03)00026-2 Marcotrigiano, 2003, Heavy metal, polychlorinated biphenyl and organochlorine pesticide residues in marine organisms: risk evaluation for consumers, Vet. Res. Commun., 27, 183, 10.1023/B:VERC.0000014137.02422.f4 Mavrakis, 2004, Distribution of trace metals in the sediments of Elefsis Gulf, Mediter. Mar. Sci., 5, 151, 10.12681/mms.220 NCMR (National Center for Marine Research), 1997. Pollution research and monitoring programme in the Saronikos Gulf: Technical Report, Athens. Orbea, 2002, Antioxidant enzymes and peroxisome proliferation in relation to contaminant body burdens of PAHs and PCBs in bivalve mollucs, crabs and fish form the Urdaibai and Plentzia estuaries (Bay of Biscay), Aquat. Toxicol., 58, 75, 10.1016/S0166-445X(01)00226-0 Pampanin, 2005, Susceptibility to oxidative stress of mussels (Mytilus galloprovincialis) in the Venice Lagoon (Italy), Mar. Pollut. Bull., 50, 1548, 10.1016/j.marpolbul.2005.06.023 Papathanasiou, E., Zenetos, A., (Eds.), 2005. State of the Hellenic Marine Environment. Hellenic Center for Marine Research, Report, Athens. Regoli, 1998, Trace metals and antioxidant enzymes in gills and digestive gland of the Mediterranean mussel Mytilus galloprovincialis, Arch. Environ. Res., 54, 419, 10.1016/S0141-1136(02)00146-0 Regoli, 1995, Gluatathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metal under field and laboratory conditions: implications for the use of biochemical biomarkers, Aquatic Toxicol., 31, 143, 10.1016/0166-445X(94)00064-W Regoli, 2002, Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach, Mar. Environ. Res., 54, 419, 10.1016/S0141-1136(02)00146-0 Regoli, 2002, Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: the relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus), Mar. Pollut. Bull., 44, 912, 10.1016/S0025-326X(02)00120-0 Sklivagou, 2001, Aliphatic and polycyclic aromatic hydrocarbons in surface sediments from the Elefsis Bay, Greece (Eastern Mediterranean), Toxicol. Environ. Chem., 79, 195, 10.1080/02772240109358988 Scoullos, 1997, Speciation studies of trace metals in the Gulf of Elefsis, Greece, Croatica Chem. Acta, 70, 299 Scoullos, 2007, Dissolved and particulate trace levels in the Saronikos Gulf, Greece, in 2004. The impact of the primary wastewater treatment plant of Psyttalia, Desalination, 210, 98, 10.1016/j.desal.2006.05.036 Soto, 2000, Changes in mussel biometry on exposure to metals: implications in estimation of metal bioavailability in “Mussel-Watch” programmes, Sci. Total Environ., 247, 175, 10.1016/S0048-9697(99)00489-1 Stephensen, 2000, Biochemical indicators of pollution exposure in shorthorn sculpin (Myoxocephalus scorpius), caught in four harbours on the southwest coast of Iceland, Aquat. Toxicol., 48, 431, 10.1016/S0166-445X(99)00062-4 Stoscheck, 1990, Quantitation of proteins, Methods Enzymol., 182, 50, 10.1016/0076-6879(90)82008-P Tsangaris, 2004, Measurements of biochemical markers of pollution in mussels Mytilus galloprovincialis from coast areas of the Saronikos Gulf, Mediter. Mar. Sci., 5, 175, 10.12681/mms.223 Valavanidis, 2006, Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants, Ecotoxicol. Environ. Safe., 46, 178, 10.1016/j.ecoenv.2005.03.013 Viarengo, 1989, Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level, CRC Rev. Aquat. Sci., 1, 295 Viarengo, 1991, Seasonal variations in the antioxidant defence systems and lipid peroxidation of the digestive gland of mussels, Compar. Biochem. Physiol., 100, 187, 10.1016/0742-8413(91)90151-I Yap, 2004, Toxicities and tolerances of Cd, Cu, Pb and Zn in primary producer (Isochrysis galbana) and in a primary consumer (Perna viridis), Environ. Int., 29, 1097, 10.1016/S0160-4120(03)00141-7 Zangrandi, 2005, The sensitivity of mussel- watch programme to detect temporal trends in the bio-availability of metals. A case study of the Saronikos Gulf of Greece, J. Environ. Monitor., 7, 798, 10.1039/b419326a