Intestinal metabolism of T-2 toxin in the pig cecum model

Mycotoxin Research - Tập 28 - Trang 191-198 - 2012
Qinghua Wu1,2, Anna Engemann3,2, Benedikt Cramer2, Tanja Welsch2, Zonghui Yuan1, Hans-Ulrich Humpf3,2
1MOA Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, People’s Republic of China
2Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany
3NRW Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany

Tóm tắt

T-2 toxin, a toxic member of the group A trichothecenes, is produced by various Fusarium species that can potentially affect human health. As the intestine plays an important role in the metabolism of T-2 toxin for animals and humans, the degradation and metabolism of T-2 toxin was studied using the pig cecum in vitro model system developed in the author’s group. In order to study the intestinal degradation of T-2 toxin by pig microbiota, incubation was performed with the cecal chyme from four different pigs in repeat determinations. A large variation in the intestinal degradation of T-2 toxin was observed for individual pigs. T-2 toxin was degraded almost completely in one out of four pigs, in which only 3.0 ± 0.1 % of T-2 toxin was left after 24 h incubation. However, in the other three incubations with pig cecal suspension, 54.1 ± 11.7–68.9 ± 16.1 % of T-2 toxin were still detectable after 24 h incubation time. The amount of HT-2 toxin was increased along with the incubation time, and HT-2 toxin accounted for 85.2 ± 0.7 % after 24 h in the most active cecum. HT-2 toxin was the only detectable metabolite formed by the intestinal bacteria. This study suggests that the toxicity of T-2 toxin for pigs is caused by the combination of T-2 and HT-2 toxins.

Tài liệu tham khảo

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