Simultaneous effect of irradiance and temperature on biochemical composition of the microalga Pavlova lutheri
Tóm tắt
The biochemical composition of microalgae can be modulated through the environmental conditions prevailing during growth. The simultaneous effects of irradiance and temperature on the biochemical composition of Pavlova lutheri were evaluated using an experimental star factorial design. Five levels were tested for each parameter (temperature, 10, 14, 18, 22 and 26°C; irradiance, 60, 105, 150, 195 and 240 μmol photons m−2 s−1), whereas the carbohydrate, protein, lipid, pigments and elementary compound contents were measured as response variables. Additionally, in order to rapidly measure parameters to define the status of the culture, the validation of the relationships between biochemical parameters and physiological status were estimated through regression analysis. It was observed that irradiance and temperature play a major role in the determination of the biochemical composition of microalgae. Their effects are synergistic, and it can be observed that a trend in behaviour at a certain temperature can be reversed at a different temperature; therefore, when selecting the environmental conditions to a culture they must be studied in a combined fashion. Although there are consistent relationships between pigment contents and elementary compounds in cells, its linearity is influenced by the irradiance of the culture and its age; therefore, they can only be applied in specific circumstances. On the other side, population biomass was well estimated in terms of carotenoid content, irrespective of the environmental conditions provided and the growth phase.
Tài liệu tham khảo
Araújo SC, Garcia VMT (2005) Growth and biochemical composition of the diatom Chaetoceros cf. wighamii brightwell under different temperature, salinity and carbon dioxide levels. I. Protein, carbohydrates and lipids. Aquacult 246:405–412
Ayala JF, Bravo BR (1984) Animal wastes media for Spirulina production. Arch Hydrobiol Suppl 67:349–355
Baeck SH, Shinji S, Kikuchi T (2008) Growth of dinoflagellates, Ceratium furca and Ceratium fusus in Sagami Bay, Japan: The role of temperature, irradiance and photoperiod. Harmful Algae 7:163–173
Bligh WJ, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Borowitzka MA (1988) Vitamins and fine chemicals from microalgae. In: Borowitzka, MA, Borowitzka, LJ (eds) Micro-algal Biotechnology. Cambridge University Press, Cambridge, pp 153–196
Carvalho AP, Malcata FX (2003) Kinetic modeling of the autotrophic growth of Pavlova lutheri: study of the combined influence of light and temperature. Biotechnol Progr 19:1128–1135
Clark DR, Merret MJ, Flynn KJ (1999) Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress. New Phytol 144:463–470
Clark DR (2001) Growth rate relationships to physiological indices of nutrient status in marine diatoms. J Phycol 37:249–256
Davidson K, Flynn KJ, Cunningham A (1991) Relationships between photopigments, cell carbon, cell nitrogen and growth rate for a marine nanoflagellate. J Exp Mar Biol Ecol 153:87–96
Del Campo JA, Moreno J, Rodriguez H, Vargas MA, Rivas J, Guerrero M (2000) Carotenoid content of chlorophycean microalgae: factors determining lutein accumulation in Muriellopsis sp. (Chlorophyta). J Biotechnol 76:51–59
Dermoun D, Chaumont D, Thebault J, Dauta A (1992) Modelling of growth of Porphyridium cruentum in connection with two interdependent factors: light and temperature. Bior Technol 42:113–117
Dubinsky Z, Matsukawa R, Karube I (1995) Photobiological aspects of algal mass culture. J Mar Biotechnol 2:61–65
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Finkel ZV, Irwin AJ, Schofield O (2004) Resource limitation alters the 3/4 size scaling of metabolic rates in phytoplankton. Mar Ecol Prog Ser 273:269–279
Fuentes MMR, Fernandez GGA, Perez JAS, Guerrero JLG (2000) Biomass nutrient profiles of the microalga Porphyridium cruentum. Food Chem 70:345–353
Harding WW, Meeson BW, Fisher TR (1985) Patterns of photosynthetic carbon metabolism in light-limited phytoplankton. Mar Biol 89:121–133
Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanz 167:191–194
Joo DS, Cho MG, Rainer B, Lee EH (1998) Growth and fatty acid composition with growth conditions for Spirulina platensis. J Korean Fish Soc 31:409–416
Jorgensen EG, Steeman-Nielsen E (1965) Adaptation in plankton algae. Ist Total Idrobiol 18S: 37–46
Jorgensen EG (1968) The adaptation of plankton algae II. Aspects of the temperature adaptation of Skeletonema costatum. Physiol Plant 21:423–427
Lowry O, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Madariaga I, Joint I (1992) A comparative study of phytoplankton physiological indicators. J. Exp Mar Biol Ecol 158:149–165
Otero A, Vincenzini M (2003) Extracellular polysaccharide synthesis by Nostoc strains as affected by N source and irradiance. J Biotechnol 102:143–152
Ponis E, Parisi G, Le Coz J-R, Zittelli C, Tredici MR (2006) Effect of the culture system and culture technique on biochemical characteristics of Pavlova lutheri and its nutritional value for Crassostrea gigas larvae. Aquac Nut 12:322–329
Richmond A (1986) CRC Handbook of Microalgal Mass Culture. CRC, Boca Raton, Florida
Rochet M, Legendre L, Demers S (1985) Acclimation of sea-ice microalgae to freezing temperature. Mar Ecol Prog Ser 24:187–191
Spektorova LV, Nosova LP, Goronkova OI, Albitskaya ON, Filippovskij Yu N (1986) High-density culture of marine microalgae—promising items for mariculture. 2. Determination of optimal light regime for Chlorella sp. marina under high-density culture conditions. Aquacult 55:221–229
Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Optimization of Nannochloropsis oculata growth using the response surface method. J Chem Technol Biotechnol 81:1049–1056
Sukenik A, Wahnon R (1991) Biochemical quality of marine unicellular algae with special emphasis on lipid composition. I. Isochrysis galbana. Aquacult 97:61–72
Sukenik A, Zmora O, Carmeli Y (1993) Biochemical quality of marine unicellular algae with special emphasis on lipid composition. I. Nannochloropsis sp. Aquacult 117:313–326
Thompson PA, Guo M (1992) Effects of variation in temperature. i. on the biochemical composition of eight species of marine phytoplankton. J Phycol 28:481–488
Thompson P (1999) The response of growth and biochemical composition to variations in daylength, temperature, and irradiance in the marine diatom Thalassiosira pseudonana (Bacillariophyceae). J Phycol 35:1215–1223
Trabelsi L, Ben Ouada H, Bacha H, Ghoul M (2009) Combined effect of temperature and irradiance on growth and extracellular polymeric substance production by the cyanobacterium Arthrospira platensis. J Appl Phycol (in press)
Valenzuela-Espinosa E, Millan-Nunez R, Trees CC, Santamaria-del-Angel E, Nunez-Cebrero F (2007) Growth and accessory pigment to chlorophyll a ratios of Thalassiosira pseudonana (Bacillariophyceae) cultured under different irradiances. Hidrobiologica 17(3):249–255
Volkman JK, Jeffrey SW, Nichols PD, Rogers GL, Garland CD (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture. J Exp Mar Biol Ecol 128:219–240
Vonshak A, Torzillo G, Boussiba S, Millie DF, Kurgens P (2000) Temperature induced photoinhibition in outdoor cultures of Monodus subterraneus. Proc. 54th Annual Meeting. Phycological Society of America, San Diego, CA