Epoxy resin as fixative during freeze-substitution

Al-Amoudi, 2004, Cryo-electron microscopy of vitreous sections, EMBO J., 23, 3583, 10.1038/sj.emboj.7600366 Al-Amoudi, 2003, An oscillating cryoknife reduces cutting-induced deformation of vitreous ultrathin sections, J. Microsc., 212, 26, 10.1046/j.1365-2818.2003.01244.x Ameye, 2000, Ultrastructure of the echinoderm cuticle after fast-freezing/freeze-substitution and conventional chemical fixations, Microsc. Res. Tech., 48, 385, 10.1002/(SICI)1097-0029(20000315)48:6<385::AID-JEMT8>3.0.CO;2-R Baumeister, 2002, Electron tomography: towards visualizing the molecular organization of the cytoplasm, Curr. Opin. Struct. Biol., 12, 679, 10.1016/S0959-440X(02)00378-0 Causton, B.E., 1985. Does the embedding chemistry interact with tissue? In: Mueller M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O’Hare 1985, pp. 209–214. Edelmann, 2002, Freeze-dried and resin-embedded biological material is well suited for ultrastructure research, J. Microsc., 207, 5, 10.1046/j.1365-2818.2002.01033.x Giddings, 2003, Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples, J. Microsc., 212, 53, 10.1046/j.1365-2818.2003.01228.x Hayat, 2000 Hohenberg, 1994, High-pressure freezing of cell suspensions in cellulose capillary tubes, J.Microsc., 175, 34, 10.1111/j.1365-2818.1994.tb04785.x Humbel, B., Mueller M., 1986. Freeze-substitution and low temperature embedding, In: Mueller, M., Becker, R.P., Boyde, A., Wolosewick, J.J. (Eds.), The Science of Biological Specimen Preparation for Microscopy and Microanalysis. Scanning Electron Microscopy, AMF O’Hare 1985, pp. 175–183. Hunziker, 1984, Cartilage ultrastructure after high-pressure freezing, freeze-substitution, and low temperature embedding. I. Chondrocyte ultrastructure-implications for the theories of mineralization and vascular invasion, J. Cell Biol., 98, 267, 10.1083/jcb.98.1.267 Jorgensen, 1995, Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans, Nature, 378, 196, 10.1038/378196a0 Kellenberger, 1987, The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions, J. Histochem. Cytochem., 35, 959, 10.1177/35.9.3302020 Magonov, 1997, Characterization of polymer surfaces with atomic force microscopy, Annu. Rev. Mater. Sci., 27, 175, 10.1146/annurev.matsci.27.1.175 Matsko, 2004, AFM of biological material embedded in epoxy resin, J. Struct. Biol., 146, 334, 10.1016/j.jsb.2004.01.010 Michel, 1992, Diamonds are a cryosectioner’s best friend, J. Microsc., 166, 43, 10.1111/j.1365-2818.1992.tb01506.x Monaghan, 1998, High-pressure freezing for immunocytochemistry, J. Microsc., 192, 248, 10.1046/j.1365-2818.1998.00387.x Monaghan, 2003, High-pressure freezing in the study of animal pathogens, J. Microsc., 212, 62, 10.1046/j.1365-2818.2003.01245.x Reynolds, 1963, The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol., 17, 208, 10.1083/jcb.17.1.208 Studer, 1995, Vitrification of articular cartilage by high-pressure freezing, J Microsc., 179, 321, 10.1111/j.1365-2818.1995.tb03648.x Van Harreveld, 1964, Electron microscopy after rapid freezing on a metal surface and substitution fixation, Anat. Rec., 149, 381, 10.1002/ar.1091490307 Wild, 2001, Enhanced resolution of membranes in cultured cells by cryoimmobilization and freeze-substitution, Microsc. Res. Tech., 53, 313, 10.1002/jemt.1098