An innovative method to obtain porous PLLA scaffolds with highly spherical and interconnected pores

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 86B Số 1 - Trang 9-17 - 2008
Cédryck Vaquette1, Céline Frochot2, Rachid Rahouadj1, Xiong Wang1
1LEMTA - Laboratoire d'Energétique et Mécanique Théorique et Appliquée (Université de Lorraine - ENSEM - ESSTIN, 2 avenue de la Forêt de Haye, TSA 60604, 54518 Vandoeuvre-les-Nancy Cedex - France)
2DCPR - Département de Chimie Physique des Réactions (1 Rue Grandville - BP 20451 54001 NANCY CEDEX - France)

Tóm tắt

Abstract

Scaffolding is an essential issue in tissue engineering and scaffolds should answer certain essential criteria: biocompatibility, high porosity, and important pore interconnectivity to facilitate cell migration and fluid diffusion. In this work, a modified solvent casting‐particulate leaching out method is presented to produce scaffolds with spherical and interconnected pores. Sugar particles (200–300 μm and 300–500 μm) were poured through a horizontal Meker burner flame and collected below the flame. While crossing the high temperature zone, the particles melted and adopted a spherical shape. Spherical particles were compressed in plastic mold. Then, poly‐L‐lactic acid solution was cast in the sugar assembly. After solvent evaporation, the sugar was removed by immersing the structure into distilled water for 3 days. The obtained scaffolds presented highly spherical interconnected pores, with interconnection pathways from 10 to 100 μm. Pore interconnection was obtained without any additional step. Compression tests were carried out to evaluate the scaffold mechanical performances. Moreover, rabbit bone marrow mesenchymal stem cells were found to adhere and to proliferate in vitro in the scaffold over 21 days. This technique produced scaffold with highly spherical and interconnected pores without the use of additional organic solvents to leach out the porogen. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

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Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 86B Số 1

9-17

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