Extracellular enzymatic activity of aquatic and aero-aquatic conidial fungi
Tóm tắt
Nineteen species of aquatic and areo-aquatic conidial fungi were tested for their ability to produce extracellular enzymes which degrade cellulose, starch, lipids, proteins and tannic acid. The cellulolytic activity was determined by using both solid and liquid media. The activity of other enzymes was examined using solid media. Two-thirds of the species were able to hydrolyze soluble cellulose (CMC) incorporated in solid and liquid media with varying degrees of activity. Extracellular culture filtrates ofAegerita candida, Helicodendron giganteum andH. tubulosum contained a Cl-Cx enzyme complex that could degrade both soluble cellulose (CMC) and crystalline cellulose (filter paper). Lipase activity was demonstrated by 11 species. Fourteen of the species showed activity for amylase and protease, but only 11 of the 16 were capable of degrading tannic acid.
Tài liệu tham khảo
Abdullah, S. K. & J. P. Fisher, 1984. Aero-aquatic fungal flora of two static water habitats in Devon. Trans. Br. mycol. Soc. 82: 361–365.
Abdullah, S. K. & J. Webster, 1980. Aquatic and aeroaquatic Hyphomycetes from Ireland. Ir. nat. J. 20: 49–55.
Abdullah, S. K., Y. Horie & S. Udagawa, 1986. New or interesting aero-aquatic conidial fungi from Japan. Nova Hedwigia 43: 507–513.
Bärlocher, F. & B. Kendrick, 1973. Fungi and food references ofGammarus psudolimnaeus. Arch. Hydrobiol. 72: 501–516.
Bärlocher, F. & B. Kendrick, 1974. Dynamics of the fungal population on leaves in a stream. Ecol. 57: 707–719.
Bärlocher, F. & B. Kendrick, 1976. Hyphomycetes as intermediaries of energy flow in streams. In ‘Recent advances in aquatic mycology’, E. B. Gareth Jones (ed.), Eleck Science. London: 435–466.
Bärlocher, F. & B. Kendrick, 1981. Role of aquatic hyphomycetes in the tropic structure of streams. In ‘The fungal community: its organization and role in the ecosystem’. D. T. Wicklow & G. C. Carroll, (ed.), Marcel Dekker Inc. New York: 743–760.
Beverwijk, A. L. Van, 1953. Helicosporous Hyphomycetes. I. Trans. Br. mycol. Soc. 36: 111–124.
Bisson, J. W. & V. J. Cabelli, 1979. Membrane filter enumeration method forClostridium perfringens. Appl. Envir. Microb. 37: 55–66.
Cochrane, V. W., 1985. Physiology of Fungi. John Wiley and Sons. Inc. New York. 524 pp.
Danninger, E., K. Messner & M. Rohr, 1979. Investigation into the biological disintegration of organic natural substances by Aquatic Hyphomycetes. ZbI. Bakt. Hyg. B. 169: 282–286.
Davidson, R. W., W. A. Campbell & D. J. Blaisdell, 1938. Differentiation of wood-decaying fungi by their reaction on gallic or tannic acid medium. Jour. Agric. Res. 57: 683–695.
Fagan, S. M. & G. L. Fergus, 1984. Extracellular enzymes of some additional fungi associated with mushroom culture. Mycopathologia 87: 67–70.
Fergus, C. L., 1969. The production of amylase by some thermophillic fungi. Mycologia 61: 1171–1175.
Fisher, J. P., 1977. New method of detecting and studying saprophytic behaviour of aero-aquatic Hyphomycetes from stagnant water. Trans. Br. mycol. Soc. 68: 407–411.
Fisher, J. P., P. D. Sharma & J. Webster, 1977. Cellulolytic ability of aero-aquatic Hyphomycetes. Trans. Br. mycol. Soc. 69: 495–496.
Fisher, J. P., R. A. Davey & J. Webster, 1983. Degradation of lignin by aquatic and aero-aquatic Hyphomycetes. Trans. Br. mycol. Soc. 80: 166–168.
Gessner, R. V., 1980. Degradative enzyme production by Salt-marsh fungi. Bot. Mar. 23: 133–139.
Hankin, L. & S. L. Anagnostakis, 1975. The use of solid media for detection of enzyme production by fungi. Mycologia 67: 597–607.
Ingold, C. T., 1942. Aquatic Hyphomycetes of decaying alder leaves. Trans. Br. mycol. Soc. 25: 339–417.
Kirk, T. K., W. J. Connors & J. G. Zeikus, 1977. Advances in understanding the microbiological degradation of lignin. In ‘Recent Advances in phytochemistry. Vol. II’ F. A. Loewus & V. C. Runeckles (eds) Plenum press. New York: 369–394.
Miller, G. L., 1959. Use of the Dinitrosalicylic acid reagent for the determination of reducing sugars. Analyt. Chem. 31: 426–428.
Padgett, D. E., 1976. Leaf decomposition by fungi in tropical rainforest streams. Biotropica 8: 166–178.
Reese, E. T. & M. Mandels, 1963. Enzymic hydrolysis of cellulose and its derivatives. In ‘Methods in carbohydrate chemistry. Vol. 3’. R. L. Whisler. (ed.) Academic press. New York: 139–143.
Sierra, G., 1957. A simple method for the detection of lipolytic activity of microorganisms and some observation on the influence of the contact between cells and fatty substrates. Antonie Van Leeuwenhoek 23: 15–22.
Singh, N., 1982. Cellulose decomposition by some tropical aquatic Hyphomycetes. Trans. Br. mycol. Soc. 79: 560–561.
Society of American Bacteriologist, 1951. Manual of Methods for Pure Culture Study of Bacteria. McGraw-Hill, New York and London.
Suberkropp, K. & M. J. Klug, 1976. Fungi and bacteria associated with leaves during processing in a woodland stream. Ecology 57: 707–719.
Suberkropp, K. & T. L. Arsuffi, 1984. Degradation, growth and changes in palatability of leaves colonized by six aquatic hyphomycete species. Mycologia 76: 398–407.
Suberkropp, K., T. L. Arsuffi & J. P. Anderson, 1983. Comparison of degradative ability, enzymatic activity, and palatability of Aquatic Hyphomycetes grown on leaf litter. Appl. Env. Microbiol. 46: 237–244.
Trigiano, R. N. & C. L. Fergus, 1979. Extracellular enzymes of some fungi associated with mushroom culture. Mycologia 71: 908–917.
Webster, J. & E. Descals, 1981. Morphology, distribution and ecology of conidial fungi in freshwater habitats. In: The Biology of conidial fungi, G. T. Cole & W. B. Kendrick (eds), New York and London, Academic Press.
Willoughby, L. B. & J. F. Archer, 1973. The fungal flora of a freshwater stream and its colonization pattern on wood. Freshwater Biology 3: 219–239.