An improved micro-method for the measurement of steroid profiles by APPI-LC–MS/MS and its use in assessing diurnal effects on steroid concentrations and optimizing the diagnosis and treatment of adrenal insufficiency and CAH

Alary, 2001, Comparative study: LC/MS/MS analysis of four steroid compounds using a new photo- ionization source and a conventional APCI source Stolze, 2015, Use of micro-HPLC–MS/MS method to assess diurnal effects on steroid hormones, Clin. Chem., 61, 556, 10.1373/clinchem.2014.232546 Joos, 1999, Liquid chromatography–tandem mass spectrometry of some anabolic steroids, Anal. Chem., 71, 4701, 10.1021/ac981073s Dorgan, 2002, Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry, Steroids, 67, 151, 10.1016/S0039-128X(01)00147-7 Chang, 2003, Quantitative measurement of male steroid hormones using automated on-line solid phase extraction-liquid chromatography–tandem mass spectrometry and comparison with radioimmunoassay, Analyst, 128, 363, 10.1039/b210111b Tang, 2001, Analysis of corticosteroids in equine urine by liquid chromatography–mass spectrometry, J. Chromatogr. B Biomed. Sci. Appl., 754, 229, 10.1016/S0378-4347(00)00613-7 Taylor, 2002, Validation of a high-throughput liquid chromatography–tandem mass spectrometry method for urinary cortisol and cortisone, Clin. Chem., 48, 1511, 10.1093/clinchem/48.9.1511 Minutti, 2004, Steroid profiling by tandem mass spectrometry improves the positive predictive value of newborn screening for congenital adrenal hyperplasia, J. Clin. Endocrinol. Metab., 89, 3687, 10.1210/jc.2003-032235 Kautsky, 1981 Abraham, 1981 Soldin, 2009, Steroid hormone analysis by tandem mass spectrometry, Clin. Chem., 55, 1061, 10.1373/clinchem.2007.100008 Guo, 2004, Steroid profiles using liquid chromatography–tandem mass spectrometry with atmospheric pressure photoionization source, Arch. Pathol. Lab. Med., 128, 469, 10.5858/2004-128-469-SPULCM Guo, 2006, Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photospray ionization tandem mass spectrometry, Clin. Chim. Acta, 372, 76, 10.1016/j.cca.2006.03.034 Holst, 2007, Use of steroid profiles in determining the cause of adrenal insufficiency, Steroids, 72, 71, 10.1016/j.steroids.2006.11.001 Howie, 2011, Blood sample volumes in child health research: review of safe limits, Bull. World Health Organ., 89, 46, 10.2471/BLT.10.080010 Debono, 2015, Hormonal circadian rhythms in patients with congential adrenal hyperplasia: identifying optimal monitoring times and novel disease biomarkers, Eur. J. Endocrinol., 173, 727, 10.1530/EJE-15-0064 Flück, 2003, The 17, 20-lyase activity of cytochrome P450c17 from human fetal testis favors the Δ5 steroidogenic pathway, Clin. Endocrinol. Metab., 88, 3762, 10.1210/jc.2003-030143 Wilson, 2002, The role of 5α-reduction in steroid hormone physiology, Reprod. Fertil. Dev., 13, 673, 10.1071/RD01074 White, 2000, Congenital adrenal hyperplasia due to 21-hydroxylase deficiency, Endocr. Rev., 21, 245 Wilson, 2003, 5α-Androstane-3α, 17β-diol is formed in tammar wallaby pouch young testes by a pathway involving 5α-pregnane-3α, 17α-diol-20-one as a key intermediate, Endocrinology, 144, 575, 10.1210/en.2002-220721 Auchus, 2004, The backdoor pathway to dihydrotestosterone, Trends Endocrinol. Metab., 15, 432, 10.1016/S1043-2760(04)00214-0 Gupta, 2003, 5α-Reduced C21 steroids are substrates for human cytochrome P450c17, Arch. Biochem. Biophys., 418, 151, 10.1016/j.abb.2003.07.003 Mahendroo, 2004, Steroid 5α-reductase 1 promotes 5α-androstane-3α,17β-diol synthesis in immature mouse testes by two pathways, Mol. Cell. Endocrinol., 222, 113, 10.1016/j.mce.2004.04.009 Kyriakopoulou, 2013, A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals, Clin. Biochem., 46, 642, 10.1016/j.clinbiochem.2013.01.002 Brambilla, 2009, The effect of diurnal variation on clinical measurement of serum testosterone and other sex hormone levels in men, J. Clin. Endocrinol. Metab., 94, 907, 10.1210/jc.2008-1902 Cooke, 1993, Circadiam variation in serum free and non-SHBG-cound testosterone in normal men: measurements, and simulation using a mass action model, Clin. Endocrinol., 39, 163, 10.1111/j.1365-2265.1993.tb01769.x Diver, 2003, Diurnal rhythms of serum total, free and bioavailable testosterone and of SHBG in middle-ages men compared with those in young men, Clin. Endocrinol., 58, 710, 10.1046/j.1365-2265.2003.01772.x Faiman, 1971, Diurnal cycles in plasma FSH, testosterone and cortisol in men, J. Clin. Endocrinol. Metab., 33, 186, 10.1210/jcem-33-2-186 Nicolau, 1985, Circadian and circannual variations of FSH, LH, testosterone, dehydroepiandrosterone-sulfate (DHEA-S0 and 17-hydroxy progesterone (17 OH-Prog) in elderly men and women, Endocrinologie, 23, 223 Winters, 1991, Diurnal rhythm of testosterone and luteinizing hormone in hypogonadal, J. Androl., 12, 185 Rose, 1978, Diurnal variation in plasma testosterone and cortisol in rhesus monkeys living in social groups, J. Endocrinol., 76, 67, 10.1677/joe.0.0760067 Gouden, 2014, Clinical use of reference intervals derived from some CALIPER studies questioned, Clin. Chem., 60, 416, 10.1373/clinchem.2013.214684