Affinity Purification of Recombinant Trypsinogen Using Immobilized Ecotin

McGrath, 1995, Ecotin: Lessons on survival in a protease-filled world, Protein Sci., 4, 141, 10.1002/pro.5560040201 Pál, 1996, Stable monomeric form of an originally dimeric serine proteinase inhibitor, ecotin, was constructed via site directed mutagenesis, FEBS Lett., 385, 165, 10.1016/0014-5793(96)00376-6 Pál, 1994, Alteration of the specificity of ecotin, anE. coli, FEBS Lett., 342, 57, 10.1016/0014-5793(94)80584-9 Chung, 1983, Purification fromEscherichia coli, J. Biol. Chem., 258, 11032, 10.1016/S0021-9258(17)44382-1 Gráf, 1987, Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin, Biochemistry, 26, 2616, 10.1021/bi00383a031