Cutting Edge: TLR2-Deficient and MyD88-Deficient Mice Are Highly Susceptible toStaphylococcus aureusInfection

Journal of Immunology - Tập 165 Số 10 - Trang 5392-5396 - 2000
Osamu Takeuchi1,2, Katsuaki Hoshino1,2, Shizuo Akira1,2
1*Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan; and
2†Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, Osaka, Japan

Tóm tắt

AbstractToll-like receptor (TLR) family acts as pattern recognition receptors for pathogen-specific molecular patterns. We previously showed that TLR2 recognizes Gram-positive bacterial components whereas TLR4 recognizes LPS, a component of Gram-negative bacteria. MyD88 is shown to be an adaptor molecule essential for TLR family signaling. To investigate the role of TLR family in host defense against Gram-positive bacteria, we infected TLR2- and MyD88-deficient mice with Staphylococcus aureus. Both TLR2- and MyD88-deficient mice were highly susceptible to S. aureus infection, with more enhanced susceptibility in MyD88-deficient mice. Peritoneal macrophages from MyD88-deficient mice did not produce any detectable levels of cytokines in response to S. aureus. In contrast, TLR2-deficient macrophages produced reduced, but significant, levels of the cytokines, and TLR4-deficient macrophages produced the same amounts as wild-type cells, indicating that S. aureus is recognized not only by TLR2, but also by other TLR family members except for TLR4.

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Journal of Immunology

Tập 165 Số 10

5392-5396

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