Microfiltration of yeast cells in an internal filter reactor
Korean Journal of Chemical Engineering - Tập 13 - Trang 404-408
Tóm tắt
Microfiltration was carried out in a newly-developed internal filter reactor system (stainless steel membrane filter, pore size=2 or 10 μm) using yeast cells ofSaccharomyces cerevisiae ATCC 24858, industrialS. cerevisiae, and recombinant yeast RH 51. The filter performance was measured in terms of filtrate flux and retention coefficient of cell, and was highly influenced by agitation speed and cell concentration. Both gel polarization model and solid flux model failed to predict the filtration behavior in the internal filter system. An empirical equation was obtained to correlate filtrate flux as a function of agitation speed and cell concentration. Retention coefficient with a filter of 2 Μm pore size was found more than 95%, and the filter was suitable for the yeast cell separation.
Tài liệu tham khảo
Chang, H. N., Lee, W. G. and Kim, B. S., “Cell Retention Culture with an Internal Filter Module: Continuous Ethanol Fermentation”,Biotechnol. Bioeng.,41, 677 (1993).
Chang, H. N., Yoo, I. K. and Kim, B. S., “High Density Cell Culture by Membrane-Based Cell Recycle“,Biotechnol. Adv.,12, 467 (1994).
Kang, B. C., Lee, S. Y. and Chang, H. N.,“Production ofBacillus thuringiensis Spores in Total Cell Retention Culture and Two-Stage Continuous Culture Using an Internal Ceramic Filter System”,Biotechnol. Bioeng.,42, 1107 (1993).
Kroner, K. H., Schutte, H., Husdedt, H. and Kula, M. R., “Cross Flow Filtration in the Down Sream Processing of Enzymes”,Proc. Biochem.,19(Apr.), 68 (1984).
Lee, C. W. and Chang, H. N.,“Kinetics of Ethanol Fermentations in Membrane Cell Recycle Fermentors”,Biotechnol. Bioeng.,29, 1105 (1987).
Lee, W. G., Lee, Y. S., Chang, H. N. and Chang, Y. K.,“A Cell Retention Internal Filter Reactor for Ethanol Production Using Tapioca Hydrolysates”Biotechnol. Techniques,8, 817 (1994).
Lee, Y. L. and Chang, H. N.,“High Cell Density Culture of a RecombinantEscherichia coli Producing Penicillin Acylase in a Membrane Cell Recycle Fermentor”,Biotechnol. Bioeng.,36, 330 (1990).
Nagata, N., Herouvis, K. J., Dziewulski, D. M. and Beifort, G., “Cross-Flow Membrane Microfiltration of Bacterial Fermentation Broth”,Biotechnol. Bioeng.,34, 447 (1989).
Patel, P. N., Mehaia, M. A. and Cheryan, M.,“Cross-Flow Membrane Filtration of Yeast Suspensions”,J. Biotechnol,5, 1 (1987).
Suzuki, T., Sato, T. and Kominami, M.,“A Dense Cell Retention Culture System Using a Stirred Ceramic Membrane Reactor”,Biotechnol. Bioeng.,44, 1186 (1994).
Tanaka, T., Kamimura, R., Itoh, K., Nakanishi, K. and Matsuno, R., “Factors Affecting the Performance of Crossflow Filtration of Yeast Cell Suspension”,Biotechnol. Bioeng.,41, 617 (1993).
Warren, R. K., Macdonald, D. G. and Hill, G. A.,“Cross-Flow Microfiltration ofSaccharomyces cerevisiae”,Proc. Biochem.,23, 337 (1991).