Systematic search for zinc‐binding proteins in Escherichia coli

FEBS Journal - Tập 269 Số 9 - Trang 2403-2413 - 2002
Akira Katayama1,2, Atsuko Tsujii2, Akira Wada3, Takeshi Nishino2, Akira Ishihama1
1National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka, Japan
2Nippon Medical School, Department of Biochemistry and Molecular Biology, Bunkyo-ku, Tokyo, Japan
3Osaka Medical School, Department of Physics, Takatsuki, Osaka, Japan

Tóm tắt

A systematic search for Escherichia coli proteins with the zinc‐binding activity was performed using the assay of radioactive Zn(II) binding to total E. coli proteins fractionated by two methods of two‐dimensional gel electrophoresis. A total of 30–40 radioactive spots were identified, of which 14 have been assigned from N‐terminal sequencing. In addition to five known zinc‐binding proteins, nine zinc‐binding proteins were newly identified including: acetate kinase (AckA), DnaK, serine hydroxymethyltransferase (GlyA), transketolase isozymes (TktA/TktB), translation elongation factor Ts (Tsf), ribosomal proteins L2 (RplB), L13 (RplM) and one of S15 (RpsO), S16 (RpsP) or S17 (RpsQ). Together with about 20 known zinc‐binding proteins, the total number of zinc‐binding proteins in E. coli increased up to more than 30 species (or more than 3% of about 1000 proteins expressed under laboratory culture conditions). The specificity and affinity of zinc‐binding were analysed for some of the zinc‐binding proteins.

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FEBS Journal

Tập 269 Số 9

2403-2413

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