Betaine aldehyde dehydrogenase from Pseudomonas aeruginosa: cloning, over-expression in Escherichia coli, and regulation by choline and salt

Archiv für Mikrobiologie - Tập 185 Số 1 - Trang 14-22 - 2006
Roberto Velasco-Garcı́a1, Miguel Villalobos2, Miguel A. Ramı́rez-Romero3, Carlos Mújica-Jiménez4, Gabriel Iturriaga5, Rosario A. Muñoz‐Clares4
1Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, México, Mexico
2Programa de Genómica Funcional de Procariotes, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, México, Mexico
3Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, México, Mexico
4Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, México, Mexico
5Departamento de Biotecnología Ambiental, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, México, Mexico

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Tài liệu tham khảo

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1989) Short protocols in molecular biology. Wiley, New York

Boch J, Kempf B, Schimd R, Bremer E (1996) Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes. J Bacteriol 178:5121–5129

Boch J, Nau-Wagner G, Kneip S, Bremer E (1997) Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine. Arch Microbiol 168:282–289

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–256

Brinkman AB, Ettema TJ, de Vos WM, van der Oost J (2003) The Lrp family of transcriptional regulators. Mol Microbiol 48:287–94

Csonka LN (1989) Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev 53:121–147

D’souza-Ault MR, Smith LT, Smith GM (1993) Roles of N-acetylglutaminyl-glutamine amide and glycine betaine in adaptation of Pseudomonas aeruginosa to osmotic stress. Appl Environ Microbiol 59:473–478

Falkenberg P, Strøm AR (1990) Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase from Escherichia coli. Biochim Biophys Acta 1034:253–259

Guzmán-Partida A, Valenzuela-Soto EM (1998) Porcine kidney betaine aldehyde dehydrogenase: purification and properties. Comp Biochem Physiol 119:485–491

Hibino T, Meng YL, Kawamitsu Y, Uehara N, Matsuda N, Tanaka Y, Ishikawa H, Baba S, Takabe T, Wada K, Ishii T, Takabe T (2001) Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh. Plant Mol Biol 45:353–363

Incharoensakdi A, Matsuda N, Hibino T, Meng YL, Ishikawa H, Hara A, Funaguma T, Takabe T, Takabe T (2000) Overproduction of spinach betaine aldehyde dehydrogenase in Escherichia coli. Structural and functional properties of wild-type, mutants and E. coli enzymes. Eur J Biochem 267:7015–7023

Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170:319–330

Kilbourn JP (1978) Bacterial content and ionic composition of sputum in cystic fibrosis. Lancet 311:334

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227:680–685

Lamark T, Kaasen EM, Eshoo W, Falkenberg P, McDougall J, Strøm AR (1991) DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol Microbiol 5:1049–1064

Lamark T, Røkenes TP, Mcdougall J, Strøm AR (1996) The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress. J Bacteriol 178:1655–1662

Levering PR, van Dijken JP, Veenhius M, Harder W (1981) Arthrobacter P1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines. Arch Microbiol 129:72–80

Lisa TA, Casale CH, Domenech CE (1994) Cholinesterase, acid phosphatase, and phospholipase C of Pseudomonas aeruginosa under hyperosmotic conditions in a high-phosphate medium. Curr Microbiol 28:71–76

Mandon K, Østerås M, Boncompagni E, Trinchant JC, Spennato G, Poggi MC, Le Redulier D (2003) The Sinorhizobium meliloti glycine betaine biosynthetic genes (betICBA) are induced by choline and highly expressed in bacteroids. Mol Plant Microbe Interact 16:709–719

Meskys R, Harris RJ, Casaite V, Basran J, Scruton NS (2001) Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp. Implications for glycine betaine catabolism. Eur J Biochem 268:3390–3398

Miller JH (1977) Experiments in molecular genetics, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 431–435

Nagasawa T, Kawabata Y, Tani Y, Ogata. K (1976) Purification and characterization of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa A-16. Agric Biol Chem 40:1743–1749

Ostroff RM, Wretlind B, Vasil ML (1989) Identification of a new phospholipase C activity by analysis of an insertional mutation in the hemolytic phospholipase C structural gene of Pseudomonas aeruginosa. J Bacteriol 169:4597–4601

Pesin SR, Candia OA (1982) Acetylcholine concentration and its role in ionic transport by the corneal epithelium. Invest Ophtalmol Vis Sci 22:651–659

Pocard J-A, Vincent N, Boncompagni E, Smith LT, Poggi MC, Le Rudulier D (1997) Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34. Microbiology 143:1369–1379

Rathinasabapathi B, McCue KF, Gage DA, Hanson AD (1996) Metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta 193:155–152

Rennick BR (1981) Renal tubule transport of organic ions. Am J Physiol 240:F83–F89

Røkenes TP, Lamark T, Strøm AR (1996) DNA-binding properties of the BetI repressor protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex formation. J Bacteriol 178:1663–1670

Sage AE, Vasil AI, Vasil ML (1997) Molecular characterization of mutants affected in the osmoprotectant-dependent induction of phospholipase C in Pseudomonas aeruginosa. Mol Microbiol 23:43–56

Serra AL, Mariscotti JF, Barra JL, Lucchesi GI, Domenech CE, Lisa AT (2002) Glycine betaine transmethylase mutant of Pseudomonas aeruginosa. J Bacteriol 184:4301–4303

Shortridge VD, Lazdunski A, Vasil ML (1992) Osmoprotectants and phosphate regulate expression of phospholipase C in Pseudomonas aeruginosa. Mol Microbiol 6:863–871

Smith LT, Pocard JA, Bernard T, Le Rudulier D (1988) Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti. J Bacteriol 170:3142–3149

Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4353

Valenzuela-Soto EM, Muñoz-Clares RA (1994) Purification and properties of betaine aldehyde dehydrogenase extracted from detached leaves of Amaranthus hypochondriacus L. subjected to water deficit. J Plant Physiol 143:145–152

Valenzuela-Soto EM, Velasco-Garcia R, Mújica-Jiménez C, Muñoz-Clares RA. (2003) Monovalent cations requirements for the stability of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa, amaranth leaves and porcine kidney. Chem-Biol Interact 143–144:139–148

Velasco-García R, González-Segura L, Muñoz-Clares RA (2000) Steady-state kinetic mechanism of NADP+- and NAD+-dependent reactions catalyzed by betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. Biochem J 352:675–683

Velasco-García R., Mújica-Jiménez C, Mendoza-Hernández G, Muñoz-Clares RA (1999) Rapid purification and properties of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa. J Bacteriol 181:1292–1300

Welsh DT (2000) Ecological significance of compatible solute accumulation by micro-organisms: From single cells to global climate. FEMS Microbiol Rev 24:263–290

Wright JR, Clements JA (1987) Metabolism and turnover of lung surfactant. Am Rev Respir Dis 136:426–444