Stabilization and improved properties of Salipaludibacillus agaradhaerens alkaline protease by immobilization onto double mesoporous core-shell nanospheres

Sharma, 2019, A review on microbial alkaline protease: an essential tool for various industrial approaches, Indust. Biotech., 1, 69, 10.1089/ind.2018.0032 Barzkar, 2020, Marine microbial alkaline protease: recent developments in biofilm n ideal choice for industrial application, Int. J. Biol. Macromol., 161, 1216, 10.1016/j.ijbiomac.2020.06.072 Dorra, 2018, Purification and characterization of a novel high molecular weight alkaline protease produced by an endophytic Bacillus halotolerans strain CT2, Int. J. Biol. Macromol., 111, 342, 10.1016/j.ijbiomac.2018.01.024 Zhang, 2018, Synthesis and continuous catalytic application of alkaline protease nanoflowers–PVA composite hydrogel, Catal. Commun., 116, 5, 10.1016/j.catcom.2018.07.015 Marathe, 2018, Isolation, partial purification, biochemical characterization and detergent compatibility of alkaline protease produced by Bacillus subtilis, Alcaligenes faecalis and Pseudomonas aeruginosa obtained from sea water samples, J. Genet. Eng. Biotechnol., 16, 39, 10.1016/j.jgeb.2017.10.001 Sittipol, 2019, Cloning, expression, purification and characterization of a thermo- and surfactant-stable protease from Thermomonospora curvata, Biocatal. Agric. Biotechnol., 19, 10.1016/j.bcab.2019.101111 Sarkar, 2020, Biocatalysis and agricultural biotechnology extraction and characterization of alkaline protease from Streptomyces sp. GS - 1 and its application as dehairing agent, Biocatal. Agric. Biotechnol., 25, 10.1016/j.bcab.2020.101590 Joshi, 2020, Development of nano-silver alkaline protease bio-conjugate depilating eco-benign formulation by utilizing potato peel based medium, Int. J. Biol. Macromol., 152, 261, 10.1016/j.ijbiomac.2020.02.251 Cipolatti, 2016, Nanomaterials for biocatalyst immobilization-state of the art and future trends, RSC Adv., 6, 104675, 10.1039/C6RA22047A Bashir, 2018, Development and characterization of cross-linked enzyme aggregates of thermotolerant alkaline protease from Bacillus licheniformis, Int. J. Biol. Macromol., 113, 944, 10.1016/j.ijbiomac.2018.03.009 Thakrar, 2019, Catalytic, thermodynamic and structural properties of an immobilized and highly thermostable alkaline protease from a haloalkaliphilic actinobacteria, Nocardiopsis alba TATA-5, Bioresour. Technol., 278, 150, 10.1016/j.biortech.2019.01.058 Feng, 2020, A novel catalytic material for hydrolyzing cow’s milk allergenic proteins: papain-Cu3(PO4)2·3H2O-magnetic nanoflowers, Food Chem., 311, 10.1016/j.foodchem.2019.125911 Yang, 2016, In situ synthesized rGO – Fe3O4 nanocomposites as enzyme immobilization support for achieving high activity recovery and easy recycling, Biochem. Eng. J., 105 (, 273, 10.1016/j.bej.2015.10.003 Sheng, 2018, Enhanced activity and stability of papain by covalent immobilization on porous magnetic nanoparticles, Int. J. Biol. Macromol., 114, 143, 10.1016/j.ijbiomac.2018.03.088 Liu, 2019, Organic-inorganic nanoflowers: from design strategy to biomedical applications, Nanoscale, 11, 17179, 10.1039/C9NR05446D Hartmann, 2013, Immobilization of enzymes on porous silicas – benefits and challenges, Chem. Soc. Rev., 42, 6277, 10.1039/c3cs60021a Kumari, 2015, Isolation and immobilization of alkaline protease on mesoporous silica and mesoporous ZSM-5 zeolite materials for improved catalytic properties, Biochem. Biophys. Reports., 2, 108, 10.1016/j.bbrep.2015.05.009 Ibrahim, 2016, Enhancement of alkaline protease activity and stability via covalent immobilization onto hollow core-mesoporous shell silica nanospheres, Int. J. Mol. Sci., 17, 184, 10.3390/ijms17020184 Ibrahim, 2016, Alkaline protease from a new halotolerant alkaliphilic Bacillus agaradhaerens strain AK-R isolated from Egyptian soda lakes, Biosci. J., 32, 1604, 10.14393/BJ-v32n1a2016-33251 Ibrahim, 2019, Alkaline serine protease from the new halotolerant alkaliphilic Salipaludibacillus agaradhaerens strain AK-R: purification and properties, 3 Biotech., 9, 1, 10.1007/s13205-019-1928-9 El-Toni, 2012, Synthesis of double mesoporous core-shell silica spheres with tunable core porosity and their drug release and cancer cell apoptosis properties, J. Colloid Interface Sci., 378, 83, 10.1016/j.jcis.2012.04.006 Zheng, 2008, Microporous and mesoporous materials amino and quaternary ammonium group functionalized mesoporous silica: an efficient ion-exchange method to remove anionic surfactant from AMS, Microporous Mesoporous Mater., 116, 299, 10.1016/j.micromeso.2008.04.016 Yang, 2011, Core-shell Ag@SiO2@mSiO2 mesoporous nanocarriers for metal-enhanced fluorescence, Chem. Commun., 47, 11618, 10.1039/c1cc15304h Shi, 2010, Superparamagnetic aminopropyl-functionalized silica core-shell microspheres as magnetically separable carriers for immobilization of penicillin G acylase, Journal of Molecular Catalysis B : Enzymatic, 63, 50, 10.1016/j.molcatb.2009.12.003 Kunitz, 1947, Crystalline soybean trypsin inhibitor, J. Gen. Phys., 30, 291, 10.1085/jgp.30.4.291 Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Song, 2012, Preparation and characterization of a thermostable enzyme (Mn-SOD) immobilized on supermagnetic nanoparticles, Appl. Microbiol. Biotechnol., 96, 123, 10.1007/s00253-011-3835-9 Nikolic, 2012, Effect of surface functionalization on synthesis of mesoporous silica core/shell particles, Microporous Mesoporous Mater., 155, 8, 10.1016/j.micromeso.2011.12.046 Zhang, 2008, Direct synthesis of highly ordered amine-functionalized mesoporous ethane-silicas, Microporous Mesoporous Mater., 109, 172, 10.1016/j.micromeso.2007.04.050 Su, 2012, Studies on the properties of graphene oxide – alkaline protease bio-composites, Bioresour. Technol., 115, 136, 10.1016/j.biortech.2011.12.085 Migneault, 2004, Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking, BioTechniques, 37, 790, 10.2144/04375RV01 Yan, 2015, Cross-linked protein crystals by glutaraldehyde and their applications, RSC Adv., 26163, 10.1039/C5RA01722J Abdeen, 2018, Nanocomposite framework of chitosan/polyvinyl alcohol/ZnO: preparation, characterization, swelling and antimicrobial evaluation, J. Mol. Liq., 250, 335, 10.1016/j.molliq.2017.12.032 Kharrat, 2011, Immobilization of Rhizopus oryzae lipase on silica aerogels by adsorption: comparison with the free enzyme, Process Biochem., 46, 1083, 10.1016/j.procbio.2011.01.029 Huang, 2015, Immobilization of cholesterol oxidase on magnetic fluorescent core-shell-structured nanoparticles, Mater. Sci. Eng. C., 57, 31, 10.1016/j.msec.2015.07.038 Ranjbakhsh, 2012, Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles, Chem. Eng. J., 179, 272, 10.1016/j.cej.2011.10.097 Barzkar, 2020, Marine microbial alkaline protease: recent developments in biofilm n ideal choice for industrial application, Int. J. Biol. Macromol., 161, 1216, 10.1016/j.ijbiomac.2020.06.072 Daoud, 2017, Characterization of thermo-solvent stable protease from Halobacillus sp. CJ4 isolated from Chott Eldjerid hypersaline lake in Tunisia, J. Basic Microbiol., 57, 104, 10.1002/jobm.201600391 Wang, 2016, Enzyme stability and activity in non-aqueous reaction systems: a mini review, Catalysts, 6, 32, 10.3390/catal6020032 Maruthiah, 2017, Investigation on production and purification of haloalkalophilic organic solvent tolerant protease from marine Shell waste and its bioconversion to chitin by aquatic Bacillus sp, Waste and Biomass Valorization., 8, 811, 10.1007/s12649-016-9636-8 George, 2014, Approach to ecofriendly leather: characterization and application of an alkaline protease for chemical free dehairing of skins and hides at pilot scale, J. Clean. Prod., 79, 249, 10.1016/j.jclepro.2014.05.046 Wu, 2015, Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp. 129-1, J. Basic Microbiol., 1427, 10.1002/jobm.201500327 Ram, 2018, Production of alkaline protease from Aspergillus oryzae isolated from seashore of Bay of Bengal, Journal of Appl. Natur, Sci, 10, 1210, 10.31018/jans.v10i4.1905 Yazid, 2016, The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: application in the hydrolysis of different protein sources, J. Molecul. Catalysis B: Enzymatic, 133, 230, 10.1016/j.molcatb.2017.01.009