Cultivation of the ubiquitous SAR11 marine bacterioplankton clade

Giovannoni, S. J., Britschgi, T. B., Moyer, C. L. & Field, K. G. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345, 60–63 (1990)

Giovannoni, S. & Rappé, M. in Microbial Ecology of the Oceans (ed. Kirchman, D. L.) 47–84 (Wiley, New York, 2000)

Hugenholtz, P., Goebel, B. M. & Pace, N. R. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J. Bacteriol. 180, 4765–4774 (1998)

Pace, N. R. A molecular view of microbial diversity and the biosphere. Science 276, 734–740 (1997)

Ward, D. M., Bateson, M. M., Weller, R. & Ruff-Roberts, A. L. Ribosomal RNA analysis of microorganisms as they occur in nature. Adv. Microb. Ecol. 12, 219–286 (1992)

Béjà, O., Spudich, E. N., Spudich, J. L., Leclerc, M. & DeLong, E. F. Proteorhodopsin phototrophy in the ocean. Nature 411, 786–789 (2001)

Stein, J. L., Marsh, T. L., Wu, K. Y., Shizuya, H. & DeLong, E. F. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J. Bacteriol. 178, 591–599 (1996)

Schut, F. et al. Isolation of typical marine bacteria by dilution culture: growth, maintenance, and characteristics of isolates under laboratory conditions. Appl. Environ. Microbiol. 59, 2150–2160 (1993)

Button, D. K., Schut, F., Quang, P., Martin, R. & Robertson, B. Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl. Environ. Microbiol. 59, 881–891 (1993)

Giovannoni, S. J., DeLong, E. F., Olsen, G. J. & Pace, N. R. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. J. Bacteriol. 170, 720–726 (1988)

Amann, R. I., Krumholz, L. & Stahl, D. A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172, 762–770 (1990)

Porter, K. G. & Feig, Y. S. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25, 943–948 (1980)

Bano, N. & Hollibaugh, J. T. Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean. Appl. Environ. Microbiol. 68, 505–518 (2002)

Yager, P. L. et al. Dynamic bacterial and viral response to an algal bloom at subzero temperatures. Limnol. Oceanogr. 46, 790–801 (2001)

Rochelle, P. A., et al. in Nucleic Acids in the Environment (eds Trevors, J. T. & van Elsas, J. D.) 219–239 (Springer, Berlin, 1995)

DeLong, E. F., Franks, D. G. & Alldredge, A. L. Phylogenetic diversity of aggregate-attached vs free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924–934 (1993)

Rappé, M. S., Kemp, P. F. & Giovannoni, S. J. Phylogenetic diversity of marine coastal picoplankton 16S rRNA genes cloned from the continental shelf off Cape Hatteras, North Carolina. Limnol. Oceanogr. 42, 811–826 (1997)

Fuhrman, J. A. & Davis, A. A. Widespread Archaea and novel Bacteria from the deep sea as shown by 16S rRNA gene sequences. Mar. Ecol. Prog. Ser. 150, 275–285 (1997)

Bahr, M., Hobbie, J. E. & Sogin, M. L. Bacterial diversity in an arctic lake: a freshwater SAR11 cluster. Aquat. Microb. Ecol. 11, 271–277 (1996)

Ducklow, H. in Microbial Ecology of the Oceans (ed. Kirchman, D. L.) 85–120 (Wiley, New York, 2000)

Davis, H. C. & Guillard, R. R. L. Relative value of ten genera of micro-organisms as food for oyster and clam larvae. USFWS Fish Bull. 58, 293–304 (1958)

Glöckner, F. O. et al. An in situ hybridization protocol for detection and identification of planktonic bacteria. System. Appl. Microbiol. 19, 403–406 (1996)

Hicks, R. E., Amann, R. I. & Stahl, D. A. Dual staining of natural bacterioplankton with 4′,6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences. Appl. Environ. Microbiol. 58, 2158–2163 (1992)

Giovannoni, S. J., DeLong, E. F., Schmidt, T. M. & Pace, N. R. Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton. Appl. Environ. Microbiol. 56, 2572–2575 (1990)

García-Martínez, J. & Rodríguez-Valera, F. Microdiversity of uncultured marine porkaryotes: the SAR11 cluster and the marine Archaea of Group I. Mol. Ecol. 9, 935–948 (2000)

Ludwig, W. et al. Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568 (1998)

Swofford, D. PAUP* 4.0 (Sinauer Associates, Sunderland, Massachusetts, 2000)