Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production

Proceedings of the National Academy of Sciences of the United States of America - Tập 100 Số 25 - Trang 15154-15159 - 2003
Vasundhara Varthakavi1, Rita M. Smith1, Stephan Bour1, Klaus Strebel1, Paul Spearman1
1Departments of Pediatrics and Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232-2581; and Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892

Tóm tắt

Human cells resist viral infections by a variety of mechanisms. Viruses must overcome host cell restrictions to successfully reproduce their genetic material. Here, we identify a host restriction to viral replication that acts at the stage of particle assembly. Viral protein U (Vpu) is an HIV-1 accessory protein that enhances particle assembly and release in most human cells, but not in simian cells. By using human-simian cell heterokaryons, we show that the inhibition of assembly in human cells is dominant. Vpu overcomes the block to assembly in human cells and in human-simian heterokaryons. The HIV-1 vpu gene may have evolved to counteract an assembly restriction that is present in human cells.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 100 Số 25

15154-15159

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