Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering
Tóm tắt
In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.
Tài liệu tham khảo
Chen G, Ushida T, Tateishi T. Scaffold design for tissue engineering. Macromol Biosci. 2002;2:67–77.
Yang S, Leong KF, Du Z, Chua CK. The design of scaffolds for use in tissue engineering. Part I. Traditional factors. Tissue Eng. 2001;7(6):679–89.
Hollister SJ. Porous scaffold design for tissue engineering. Nat Mater. 2005;4(7):518–24.
Patrícia BM, Gabriela AS, Rui LR. Natural–origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv Drug Deliv Rev. 2007;59:207–33.
Podporska-Carroll J, Ip JWY, Gogolewski S. Biodegradable poly (ester urethane) urea scaffolds for tissue engineering: interaction with osteoblast-like MG-63 cells. Acta Biomater. 2014;10(6):2781–91.
Formhals A. US Patent No. (1934)1,975,504.
Haghi AK. Electrospun nanofiber process control. Cell Chem Technol. 2010;44(9):343–52.
Li D, Xia Y. Electrospinning of nanofibers: reinventing the wheel? Adv Mater. 2004;16(14):1151–70.
Huang W, Zou T, Li S, Jing J, Xia X, Liu X. Drug-loaded zein nanofibers prepared using a modified coaxial electrospinning process. AAPS PharmSciTech. 2013;14(2):675–81.
Ju YM, San Choi J, Atala A, Yoo JJ, Lee SJ. Bilayered scaffold for engineering cellularized blood vessels. Biomaterials. 2010;31(15):4313–21.
Samprasit W, Rojanarata T, Akkaramongkolporn P, Ngawhirunpat T, Kaomongkolgit R, Opanasopit P. Fabrication and In Vitro/In Vivo Performance of Mucoadhesive Electrospun Nanofiber Mats Containing α-Mangostin. AAPS PharmSciTech, 2015;1-13.
You Y, Min BM, Lee SJ, Lee TS, Park WH. In vitro degradation behavior of electrospun polyglycolide, polylactide, and poly (lactide‐co‐glycolide). J Appl Polym Sci. 2005;95:193–200.
You Y, Youk JH, Lee SW, Min BM, Lee SJ, Park WH. Preparation of porous ultrafine PGA fibers via selective dissolution of electrospun PGA/PLA blend fibers. Mater Lett. 2006;60(6):757–60.
Asawahame C, Sutjarittangtham K, Eitssayeam S, Tragoolpua Y, Sirithunyalug B, Sirithunyalug J. Antibacterial activity and inhibition of adherence of Streptococcus mutans by propolis electrospun fibers. AAPS PharmSciTech. 2015;16(1):82–191.
Kim K, Lu YK, Chang C, Fang D, Hsiao BS, Chu B, et al. Incorporation and controlled release of a hydrophilic antibiotic using poly (lactide-co-glycolide)-based electrospun nanofibrous scaffolds. J Controlled Release. 2004;98(1):47–56.
Ghosal K, Thomas S, Kalarikkal N, Gnanamani A. Collagen coated electrospun polycaprolactone (PCL) with titanium dioxide (TiO2) from an environmentally benign solvent: preliminary physico-chemical studies for skin substitute. J Polym Res. 2014;21(5):1–5.
Yao C, Li X, Neoh KG, Shi Z, Kang ET. Surface modification and antibacterial activity of electrospun polyurethane fibrous membranes with quaternary ammonium moieties. J Membr Sci. 2008;320(1):259–67.
Nicknejad ET, Ghoreishi SM, Habibi N. Electrospinning of Cross-Linked Magnetic Chitosan Nanofibers for Protein Release. AAPS PharmSciTech. 2015:1-7.
Subramanian G, Bialorucki C, Yildirim-ayan E. Nanofibrous yet injectable polycaprolactone-collagen bone tissue scaffold with osteoprogenitor cells and controlled release of bone morphogenetic protein-2. Mater Sci Eng C. 2015;51:16–27.
Gautam S, Chou CF, Dinda AK, Potdar PD, Mishra NC. Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Mater Sci Eng C. 2014;34:402–9.
Ko YM, Choi DY, Jung SC, Kim BH. Characteristics of plasma treated electrospun polycaprolactone (PCL) nanofiber scaffold for bone tissue engineering. J Nanosci Nanotech. 2015;15:192–5.
Chong LH, Lim MM, Sultana N. Polycaprolactone (PCL)/gelatin (Ge)-based electrospun nanofibers for tissue engineering and drug delivery application. Appl Mech Mater. 2014;554:57–61.
Lou T, Leung M, Wang X, Chang JYF, Tsao CT, Sham JGC, et al. Bi-layer scaffold of chitosan/PCL-nanofibrous mat and PLLA-microporous disc for skin tissue engineering. J Biomed Nanotechnol. 2014;10:1105–13.
Du Y, Xiaofeng C, Koh YH, Lei BO. Facilely fabricating PCL nanofibrous scaffolds with hierarchical pore structure for tissue engineering. Mater Lett. 2014;122:62–5.
Gomes SR, Rodrigues G, Martins GG, Roberto MA, Mafra M, Henriques CMR. In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: a comparative study. Mater Sci Eng C. 2015;46:348–58.
Chandika P, Ko SC, Oh GW, Heo SY, Nguyen VT, Jeon YJ, et al. Fish collagen/alginate/chitooligosaccharides integrated scaffold for skin tissue regeneration application. Int J Bio Macromol. 2015;81:504–13.
Dunn MG, Bellincampi LD, Tria AJ, Zawadsky JP. Preliminary development of a collagen-PLA composite for ACL reconstruction. J Appl Polym Sci. 1997;63:1423–8.
Lee JJ, Yu HS, Hong SJ, Jeong I, Jang JH, Kim HW. Nanofibrous membrane of collagen-polycaprolactone for cell growth and tissue regeneration. J Mater Sci Mater Med. 2009;20:1927–35. doi:10.1007/s10856-009-3743-z.
Chen G, Ushida T, Tetsuya T. Hybrid biomaterials for tissue engineering: a preparative method for PLA or PLGA–collagen hybrid sponges. Adv Mater. 2000;12:455–7.
Zhang YZ, Venugopal J, Huang ZM, Lim CT, Ramakrishna S. Characterization of the surface biocompatibility of the electrospun PCL-collagen nanofibers using fibroblasts. Biomacromolecules. 2005;6(5):2583–9.
Chakrapani VY, Gnanamani A, Giridev VR, Madhusoothanan M, Sekaran G. Electrospinning of type I collagen and PCL nanofibers using acetic acid. J Appl Polym Sci. 2012;125(4):3221–7.
Kamyar SM. Green synthesis of silver/montmorillonite/chitosan bionanocomposites using the UV irradiation method and evaluation of antibacterial activity. Int J Nanomedicine. 2010;5:875–87.
Zhuanga XC. Electrospun chitosan/gelatin nanofibers containing silver nanoparticles. Carbohydr Polym. 2010;82:524–7.
Jao WC, Yang MC, Lin CH, Hsu CC. Fabrication and characterization of electrospun silk fibroin/TiO2 nanofibrous mats for wound dressings. Polymer Adv Tech. 2012;23(7):1066–76.
Ghosal K, Latha MS, Thomas S. Poly (ester amides)(PEAs)—scaffold for tissue engineering applications. Eur Polym J. 2014;60:58–68.
Reddy PR, Varaprasad K, Sadiku R, Ramam K, Reddy GVS, Raju KM, et al. Development of gelatin based inorganic nanocomposite hydrogels for inactivation of bacteria. J Inorg Organomet Polym Mater. 2013;23(5):1054–60.
Boyan BD, Hummert TW, Dean DD, Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials. 1996;17:137–46.
Sanders JE, Stiles CE, Hayes CL. Tissue response to single-polymer fibers of varying diameters: evaluation of fibrous encapsulation and macrophage density. J Biomed Mater Res. 2000;52:231–7.
Wan H, Williams RL, Doherty PJ, Williams DF. A study of cell behavior on the surfaces of multifilament materials. J Mater Sci Mater Med. 1997;8:45–51.
Bölgen N, Menceloğlu YZ, Acatay K, Vargel I, Pişkin E. In vitro and in vivo degradation of non-woven materials made of poly (ε-caprolactone) nanofibers prepared by ectrospinning under different condition. J Biomater Sci Polym Ed. 2005;16(12):1537–55.
Demir MM, Gulgun MA, Menceloglu YZ, Erman B, Abramchuk SS, Makhaeva EE, et al. Palladium nanoparticles by electrospinning from poly (acrylonitrile-co-acrylic acid)-PdCl2 solutions. Relations between preparation conditions, particle size, and catalytic activity. Macromolecules. 2004;37(5):1787–92.
Nottelet B, Pektok E, Mandracchia D, Tille JC, Walpoth B, Gurny R, et al. Factorial design optimization and in vivo feasibility of poly(ε-caprolactone)-micro- and nanofiber-based small diameter vascular grafts. J Biomed Mater Res. 2009;89A:865–75. doi:10.1002/jbm.a.32023.
Megelski S, Stephens JS, Chase DB, Rabolt JF. Micro-and nanostructured surface morphology on electrospun polymer fibers. Macromolecules. 2002;35(22):8456–66.
Bedford NM, Steckl AJ. Photocatalytic self-cleaning textile fibers by coaxial electrospinning. ACS Appl Mater Interfaces. 2010;2(8):2448–55.
Prabhakaran MP, Venugopal JR, Ramakrishna S. Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substrates for nerve tissue engineering. Biomaterials. 2009;30(28):4996–5003.
Shalumon KT, Anulekha KH, Chennazhi KP, Tamura H, Nair SV, Jayakumar R. Fabrication of chitosan/poly (caprolactone) nanofibrous scaffold for bone and skin tissue engineering. Int J Biol Macromol. 201;48(4):571-576.
Zhang Y, Ouyang H, Lim CT, Ramakrishna S, Huang ZM. Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J Biomed Mater Res. 2005;72B:156–65. doi:10.1002/jbm.b.30128].
Pant HR, Bajgai MP, Nam KT, Seo YA, Pandeya DR, Hong ST, et al. Electrospun nylon-6 spider-net like nanofiber mat containing TiO 2 nanoparticles: a multifunctional nanocomposite textile material. J Hazard Mater. 2011;185(1):124–30.
Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani MH, Ramakrishna S. Electrospun poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials. 2008;29(34):4532–9.
Guo Z, Pereira T, Choi O, Wang Y, Hahn HT. Surface functionalized alumina nanoparticle filled polymeric nanocomposites with enhanced mechanical properties. J Mater Chem. 2006;16(27):2800–8.
Francis L, Giunco F, Balakrishnan A, Marsano E. Synthesis, characterization and mechanical properties of nylon–silver composite nanofibers prepared by electrospinning. Curr Appl Phys. 2010;10(4):1005–8.
Jeon HJ, Kim JS, Kim TG, Kim JH, Yu WR, Youk JH. Preparation of poly (ɛ-caprolactone)-based polyurethane nanofibers containing silver nanoparticles. Appl Surf Sci. 2008;254(18):5886–90.
Li SC, Li YN. Mechanical and antibacterial properties of modified nano-ZnO/high-density polyethylene composite films with a low doped content of nano-ZnO. J Appl Polym Sci. 2010;116:2965–9.