Development of a Specific Detection Technique for Cowpea Golden Mosaic Virus

Whitefly transmitted begomoviruses are on increase in tropical and subtropical countries. It is especially true of yellow mosaic viruses infecting legumes. Management of viral diseases requires specific detection techniques to demonstrate their presence in several other alternative and weed hosts. With this in view, nucleic acid hybridization test to detect Cowpea golden mosaic virus (CPGMV) in many crops and weed species was developed. This study showed that while probe to CPGMV DNA A detected all the begomoviruses, DNA B probe was highly specific and detected only CPGMV showing its specific nature.