CopA: An <i>Escherichia coli</i> Cu(I)-translocating P-type ATPase

Proceedings of the National Academy of Sciences of the United States of America - Tập 97 Số 2 - Trang 652-656 - 2000
Christopher Rensing1, Baochao Fan1, Rakesh Sharma1, Bharati Mitra1, Barry P. Rosen1
1Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201

Tóm tắt

The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli . The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae . Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.

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Tài liệu tham khảo

10.1093/jn/129.7.1251

10.1126/science.284.5415.805

10.1007/PL00006286

10.1006/bbrc.1999.1073

10.1128/JB.181.19.5891-5897.1999

10.1038/ng0193-7

10.1038/ng1293-327

10.1002/yea.320110310

10.1016/S0021-9258(18)31455-8

10.1073/pnas.91.20.9651

10.1111/j.1574-6968.1996.tb08575.x

K Melchers, L Herrmann, F Mauch, D Bayle, D Heuermann, T Weitzenegger, A Schuhmacher, G Sachs, R Haas, G Bode, et al. Acta Physiol Scand Suppl 643, 123–135 (1998).

10.1074/jbc.274.36.25827

10.1126/science.277.5331.1453

10.1111/j.1365-2958.1997.mmi518.x

10.1073/pnas.94.26.14326

10.1139/o98-071

J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, 2nd Ed., Plainview, NY, 1989).

10.1016/0092-8674(91)90532-4

B J Bachmann Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, eds F C Neidhardt, J L Ingraham, K B Low, B Magasanik, M Schaechter, H E Umbarger (American Society for Microbiology, Washington, D.C.), pp. 1190–1219 (1987).

10.1016/0003-2697(85)90442-7

10.1038/227680a0

10.1006/abbi.1994.1256

10.1146/annurev.micro.50.1.753

10.1038/5545

10.1046/j.1365-2958.1998.00883.x

10.1016/S0014-5793(98)01059-X

10.1074/jbc.270.16.9217

10.1074/jbc.274.31.22008

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 97 Số 2

652-656

Thông tin tác giả