CopA: An Escherichia coli Cu(I)-translocating P-type ATPase

Proceedings of the National Academy of Sciences of the United States of America - Tập 97 Số 2 - Trang 652-656 - 2000
Christopher Rensing1, Baochao Fan1, Rakesh Sharma1, Bharati Mitra1, Barry P. Rosen1
1Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201

Tóm tắt

The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli . The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae . Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 97 Số 2

652-656

Thông tin tác giả