Automated purification of recombinant proteins: Combining high-throughput with high yield
Tài liệu tham khảo
Moy, 2004, Genome-scale expression of proteins from Bacillus subtilis, J. Struct. Funct. Genomics, 5, 103, 10.1023/B:JSFG.0000029203.42187.20
Luan, 2004, High-throughput expression of C. elegans proteins, Genome Res., 14, 2102, 10.1101/gr.2520504
Braun, 2003, High throughput protein production for functional proteomics, Trends Biotechnol., 21, 383, 10.1016/S0167-7799(03)00189-6
Sawasaki, 2002, A cell-free protein synthesis system for high-throughput proteomics, Proc. Natl. Acad. Sci. USA, 99, 14652, 10.1073/pnas.232580399
Baneyx, 1999, Recombinant protein expression in Escherichia coli, Curr. Opin. Biotechnol., 10, 411, 10.1016/S0958-1669(99)00003-8
Terpe, 2003, Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems, Appl. Microbiol. Biotechnol., 60, 523, 10.1007/s00253-002-1158-6
Braun, 2002, Proteome-scale purification of human proteins from bacteria, Proc. Natl. Acad. Sci. USA, 99, 2654, 10.1073/pnas.042684199
Scheich, 2003, An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography, BMC Biotechnol., 3, 12, 10.1186/1472-6750-3-12
Nielsen, 1999, Machine learning approaches for the prediction of signal peptides and other protein sorting signals, Protein Eng., 12, 3, 10.1093/protein/12.1.3
Sonnhammer, 1998, A hidden Markov model for predicting transmembrane helices in protein sequences, Proc. Int. Conf. Intell. Syst. Mol. Biol., 6, 175
Yoon, 2002, Express primer tool for high-throughput gene cloning and expression, Biotechniques, 33, 1328, 10.2144/02336bc03
Dieckman, 2002, High throughput methods for gene cloning and expression, Protein Expr. Purif., 25, 1, 10.1006/prep.2001.1602
Stols, 2002, A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site, Protein Expr. Purif., 25, 8, 10.1006/prep.2001.1603
Woestenenk, 2004, His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors, J. Struct. Funct. Genomics, 5, 217, 10.1023/B:jsfg.0000031965.37625.0e
Shih, 2002, High-throughput screening of soluble recombinant proteins, Protein Sci., 11, 1714, 10.1110/ps.0205202
Hammarstrom, 2002, Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli, Protein Sci., 11, 313, 10.1110/ps.22102