Expression, purification, and characterization of avian Thy-1 from Lec1 mammalian and Tn5 insect cells

Varki, 1993, Biological roles of oligosaccharides: all of the theories are correct, Glycobiology, 3, 97, 10.1093/glycob/3.2.97 Howard, 1991, Oligosaccharides at each glycosylation site make structure-dependent contributions to biological properties of human tissue plasminogen activator, Glycobiology, 1, 411, 10.1093/glycob/1.4.411 Kornfeld, 1985, Assembly of asparagine-linked oligosaccharides, Annu. Rev. Biochem., 54, 631, 10.1146/annurev.bi.54.070185.003215 Paulson, 1989, Glycoproteins—What are the sugar chains for?, Trends Biochem. Sci., 14, 272, 10.1016/0968-0004(89)90062-5 Kobata, 2000, A journey to the world of glycobiology, Glycoconj. J., 17, 443, 10.1023/A:1011006122704 Aoki, 1995, Phenotypic alteration of carbohydrate antigens in gynecological malignancy, Acta Histochem. Cytochem., 28, 197, 10.1267/ahc.28.197 Stanley, 1989, Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity, Mol. Cell. Biol., 9, 377, 10.1128/MCB.9.2.377 Kuroda, 1990, The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector, Virology, 174, 418, 10.1016/0042-6822(90)90095-9 Mansour, 1987, Thy-1 evolution, Dev. Comp. Immunol., 11, 3, 10.1016/0145-305X(87)90003-6 Campbell, 1981, Rat brain Thy-1 glycoprotein, Biochem. J., 95, 15, 10.1042/bj1950015 Carlsson, 1983, Purification and characterization of the mouse thymocyte Thy-1 glycoprotein, Biochem. J., 211, 641, 10.1042/bj2110641 McKenzie, 1981, Biochemical characterization including amino-acid and carbohydrate compositions of canine and human-brain Thy-1 antigen, Biochem. J., 197, 629, 10.1042/bj1970629 Parekh, 1987, Tissue-specific N-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat Thy-1, EMBO J., 6, 1233, 10.1002/j.1460-2075.1987.tb02359.x Williams, 1993, Comparative analysis of the N-glycans of rat, mouse, and human Thy-1. Site-specific oligosaccharide patterns of neural Thy-1, a member of the immunoglobulin superfamily, Glycobiology, 3, 339, 10.1093/glycob/3.4.339 Reif, 1964, Akr thymic antigen: its distribution in leukemias and nervous tissues, J. Exp. Med., 120, 413, 10.1084/jem.120.3.413 Tiveron, 1992, Selective inhibition of neurite outgrowth on mature astrocytes by Thy-1 glycoprotein, Nature, 355, 745, 10.1038/355745a0 Mahanthappa, 1992, Thy-1 involvement in neurite outgrowth—perturbation by antibodies, phospholipase-C, and mutation, Dev. Biol., 150, 47, 10.1016/0012-1606(92)90006-3 Leyton, 2001, Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites, Curr. Biol., 11, 1028, 10.1016/S0960-9822(01)00262-7 Dowsing, 1992, Molecular cloning and primary structure of avian Thy-1 glycoprotein, Brain Res. Mol. Brain Res., 14, 250, 10.1016/0169-328X(92)90180-J Tessier, 1991, Enhanced secretion from insect cells of a foreign protein fused to honeybee melittin signal peptide, Gene, 98, 177, 10.1016/0378-1119(91)90171-7 Walia, 1998, Histone acetylation is required to maintain the unfolded nucleosome structure associated with transcribing DNA, J. Biol. Chem., 273, 14516, 10.1074/jbc.273.23.14516 Yu, 2000, Transcriptional repression by blimp-1 (PRDI-BF1) involves recruitment of histone deacetylase, Mol. Cell. Biol., 20, 2592, 10.1128/MCB.20.7.2592-2603.2000 Gill, 1989, Calculation of protein extinction coefficients from amino acid sequence data, Anal. Biochem., 182, 319, 10.1016/0003-2697(89)90602-7 Towbin, 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350, 10.1073/pnas.76.9.4350 Sreerama, 2000, Estimation of protein secondary structure from circular dichroism spectra: Comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set, Anal. Biochem., 287, 252, 10.1006/abio.2000.4880 Emmett, 1994, Micro-electrospray mass-spectrometry—ultra-high-sensitivity analysis of peptides and proteins, J. Am. Soc. Mass Spectrom., 5, 605, 10.1016/1044-0305(94)85001-1 Chowdhury, 1990, An electrospray-ionization mass-spectrometer with new features, Rapid Commun. Mass Spectrom., 4, 81, 10.1002/rcm.1290040305 Senko, 1997, External accumulation of ions for enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry, J. Am. Soc. Mass Spectrom., 8, 970, 10.1016/S1044-0305(97)00126-8 Senko, 1996, Electrospray ionization Fourier transform ion cyclotron resonance at 9.4T, Rapid Commun. Mass Spectrom., 10, 1824, 10.1002/(SICI)1097-0231(199611)10:14<1824::AID-RCM695>3.0.CO;2-E Senko, 1996, A high-performance modular data system for Fourier transform ion cyclotron resonance mass spectrometry, Rapid Commun. Mass Spectrom., 10, 1839, 10.1002/(SICI)1097-0231(199611)10:14<1839::AID-RCM718>3.0.CO;2-V Ledford, 1984, Space charge effects in Fourier transform mass spectrometry: mass calibration, Anal. Chem., 56, 2744, 10.1021/ac00278a027 Shi, 2000, Comparison and interconversion of the two most common frequency-to-mass calibration functions for Fourier transform ion cyclotron resonance mass spectrometry, Int. J. Mass Spectrom., 196, 591, 10.1016/S1387-3806(99)00226-2 Håkansson, 2001, Electron capture dissociation and infrared multiphoton dissociation MS/MS of an N-glycosylated tryptic peptide to yield complementary sequence information, Anal. Chem., 73, 4530, 10.1021/ac0103470 Little, 1994, Infrared multiphoton dissociation of large multiply-charged ions for biomolecule sequencing, Anal. Chem., 66, 2809, 10.1021/ac00090a004 Tatusova, 1999, BLAST 2 SEQUENCES, a new tool for comparing protein and nucleotide sequences, FEMS Microbiol. Lett., 174, 247, 10.1111/j.1574-6968.1999.tb13575.x Wilkins, 1997, Detailed peptide characterization using Peptidemass—a world-wide-web-accessible tool, Electrophoresis, 18, 403, 10.1002/elps.1150180314 Cooper, 2001, GlycoMod—a software tool for determining glycosylation compositions from mass spectrometric data, Proteomics, 1, 340, 10.1002/1615-9861(200102)1:2<340::AID-PROT340>3.0.CO;2-B Seki, 1985, A hydrophopbic transmembrane segment at the carboxy terminus of Thy-1, Science, 227, 649, 10.1126/science.2857501 Seki, 1985, The human Thy-1 gene: structure and chromosomal location, Proc. Natl. Acad. Sci. USA, 82, 6657, 10.1073/pnas.82.19.6657 Crockett, 1990, High-level expression of tissue inhibitor of metalloproteinase in Chinese hamster ovary cells using glutamine synthetase gene amplification, Biotechnology, 8, 662, 10.1038/nbt0790-662 O’Reilly, 1994 French, 1986, Partial characterization of chicken Thy-1 glycoprotein by monoclonal antibodies, J. Neurosci. Res., 16, 479, 10.1002/jnr.490160304 Haupts, 1998, Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy, Proc. Natl. Acad. Sci. USA, 95, 13573, 10.1073/pnas.95.23.13573 Campbell, 1979, Structural similarities between Thy-1 antigen from rat-brain and immunoglobulin, Nature, 282, 341, 10.1038/282341a0 Rostas, 1983, The purification and characterization of a Thy-1-like glycoprotein from chicken brain, Biochem. J., 213, 143, 10.1042/bj2130143 Sorensen, 1991, Fragmentation of proteins by S. aureus strain V8 protease. Ammonium bicarbonate strongly inhibits the enzyme but does not improve the selectivity for glutamic acid, FEBS Lett., 294, 195, 10.1016/0014-5793(91)80667-R Marshall, 1985, Taillored excitation for Fourier transform ion cyclotron resonance mass spectrometry, J. Am. Chem. Soc., 107, 7893, 10.1021/ja00312a015 Guan, 1996, Stored waveform inverse Fourier transform (SWIFT) ion excitation in trapped-ion mass spectrometry: theory and applications, Int. J. Mass Spectrom. Ion Process., 158, 5, 10.1016/S0168-1176(96)04461-8 Ingraham, 1986, The mouse Thy-1.2 glycoprotein gene—complete sequence and identification of an unusual promoter, J. Immunol., 136, 1482, 10.4049/jimmunol.136.4.1482 Cohen, 1981, Analysis of structural similarities between brain Thy-1 antigen and immunoglobulin domains, Evidence for an evolutionary relationship and a hypothesis for its functional significance, Biochem. J., 195, 31, 10.1042/bj1950031 Ailor, 1998, Overexpression of a cytosolic chaperone to improve solubility and secretion of a recombinant IgG protein in insect cells, Biotechnol. Bioeng., 58, 196, 10.1002/(SICI)1097-0290(19980420)58:2/3<196::AID-BIT12>3.0.CO;2-B Hsu, 1996, Rescue of immunoglobulins from insolubility is facilitated by PDI in the baculovirus expression system, Protein Expr. Purif., 7, 281, 10.1006/prep.1996.0040 Rudd, 1999, Oligosaccharide analysis and molecular modeling of soluble forms of glycoproteins belonging to the Ly-6, scavenger receptor, and immunoglobulin superfamilies expressed in Chinese hamster ovary cells, Glycobiology, 9, 443, 10.1093/glycob/9.5.443 Lin, 1994, Core fucosylation of high-mannose-type oligosaccharides in GlcNAc transferase I-deficient (Lec1) CHO cells, Glycobiology, 4, 895, 10.1093/glycob/4.6.895