Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS?

Acuna-Castroviejo, 2001, Melatonin, mitochondria, and cellular bioenergetics, J. Pineal Res., 30, 65, 10.1034/j.1600-079X.2001.300201.x Azziz, 2004, The prevalence and features of the polycystic ovary syndrome in an unselected population, J. Clin. Endocrinol. Metab., 89, 2745, 10.1210/jc.2003-032046 Brzezinski, 1987, Melatonin in human preovulatory follicular fluid, J. Clin. Endocrinol. Metab., 64, 865, 10.1210/jcem-64-4-865 Cha, 1998, Maturation in vitro of immature human oocytes for clinical use, Hum. Reprod. Update, 4, 103, 10.1093/humupd/4.2.103 Cha, 1991, Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program, Fertil. Steril., 55, 109, 10.1016/S0015-0282(16)54068-0 Cha, 2000, Pregnancies and deliveries after in vitro maturation culture followed by in vitro fertilization and embryo transfer without stimulation in women with polycystic ovary syndrome, Fertil. Steril., 73, 978, 10.1016/S0015-0282(00)00422-2 Cha, 2005, Obstetric outcome of patients with polycystic ovary syndrome treated by in vitro maturation and in vitro fertilization-embryo transfer, Fertil. Steril., 83, 1461, 10.1016/j.fertnstert.2004.11.044 Chattoraj, 2005, Melatonin accelerates maturation inducing hormone (MIH): induced oocyte maturation in carps, Gen. Comp. Endocrinol., 140, 145, 10.1016/j.ygcen.2004.10.013 Chian, 1999, Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin, Fertil. Steril., 72, 639, 10.1016/S0015-0282(99)00323-4 Chian, 2000, Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome, Hum. Reprod., 15, 165, 10.1093/humrep/15.1.165 Combelles, 2009, Could oxidative stress influence the in-vitro maturation of oocytes?, Reprod. Biomed. Online, 18, 864, 10.1016/S1472-6483(10)60038-7 El-Raey, 2011, Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle, Mol. Reprod. Dev., 78, 250, 10.1002/mrd.21295 Harris, 2010, Metabolism and karyotype analysis of oocytes from patients with polycystic ovary syndrome, Hum. Reprod., 25, 2305, 10.1093/humrep/deq181 Kang, 2009, Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells, J. Pineal Res., 46, 22, 10.1111/j.1600-079X.2008.00602.x Livak, 2001, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method, Methods, 25, 402, 10.1006/meth.2001.1262 Nakamura, 2003, Increased endogenous level of melatonin in preovulatory human follicles does not directly influence progesterone production, Fertil. Steril., 80, 1012, 10.1016/S0015-0282(03)01008-2 Plachot, 2003, Oocyte and embryo quality in polycystic ovary syndrome, Gynecol. Obstet. Fertil., 31, 350, 10.1016/S1297-9589(03)00059-6 Reiter, 1980, The pineal and its hormones in the control of reproduction in mammals, Endocr. Rev., 1, 109, 10.1210/edrv-1-2-109 Reiter, 1991, Melatonin: the chemical expression of darkness, Mol. Cell. Endocrinol., 79, C153, 10.1016/0303-7207(91)90087-9 Reiter, 2010, Melatonin: a multitasking molecule, Prog. Brain Res., 181, 127, 10.1016/S0079-6123(08)81008-4 Requena, 2009, The impact of in-vitro maturation of oocytes on aneuploidy rate, Reprod. Biomed. Online, 18, 777, 10.1016/S1472-6483(10)60026-0 Ronnberg, 1990, Circadian and seasonal variation in human preovulatory follicular fluid melatonin concentration, J. Clin. Endocrinol. Metab., 71, 492, 10.1210/jcem-71-2-493 2004, Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome, Fertil. Steril., 81, 19, 10.1016/j.fertnstert.2003.10.004 Roy, 1987, In vitro steroidogenesis by primary to antral follicles in the hamster during the periovulatory period: effects of follicle-stimulating hormone, luteinizing hormone, and prolactin, Biol. Reprod., 37, 39, 10.1095/biolreprod37.1.39 Shi, 2009, Melatonin exists in porcine follicular fluid and improves in vitro maturation and parthenogenetic development of porcine oocytes, J. Pineal Res., 47, 318, 10.1111/j.1600-079X.2009.00717.x Tamura, 2009, Melatonin and the ovary: physiological and pathophysiological implications, Fertil. Steril., 92, 328, 10.1016/j.fertnstert.2008.05.016 Trounson, 1994, In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients, Fertil. Steril., 62, 353, 10.1016/S0015-0282(16)56891-5 Vanecek, 1995, Melatonin inhibits increase of intracellular calcium and cyclic AMP in neonatal rat pituitary via independent pathways, Mol. Cell. Endocrinol., 107, 149, 10.1016/0303-7207(94)03437-X Woo, 2001, Direct action of melatonin in human granulosa-luteal cells, J. Clin. Endocrinol. Metab., 86, 4789, 10.1210/jc.86.10.4789 Yie, 1995, Melatonin receptors on human granulosa cell membranes, J. Clin. Endocrinol. Metab., 80, 1747, 10.1210/jc.80.5.1747