Association of Thioautotrophic Bacteria with Deep-Sea Sponges
Tóm tắt
We investigated microorganisms associated with a deep-sea sponge, Characella sp. (Pachastrellidae) collected at a hydrothermal vent site (686 m depth) in the Sumisu Caldera, Ogasawara Island chain, Japan, and with two sponges, Pachastrella sp. (Pachastrellidae) and an unidentified Poecilosclerida sponge, collected at an oil seep (572 m depth) in the Gulf of Mexico, using polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) directed at bacterial 16S rRNA gene sequences. In the PCR-DGGE profiles, we detected a single clearly dominant band in each of the Characella sp. and the unidentified Poecilosclerida sponge. BLAST search of their sequences showed that they were most similar (>99% identity) to those of the gammaproteobacterial thioautotrophic symbionts of deep-sea bivalves from hydrothermal vents, Bathymodiolus spp. Phylogenetic analysis of the near-full length sequences of the 16S rRNA genes cloned from the unidentified Poecilosclerida sponge and Characella sp. confirmed that they were closely related to thioautotrophic symbionts. Although associations between sponges and methanotrophic bacteria have been reported previously, this is the first report of a possible stable association between sponges and thioautotrophic bacteria.
Tài liệu tham khảo
Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acid Res 25:3389–3402
Distel DL, Lee HKW, Cavanaugh CM (1995) Intracellular coexistence of methanotrophic and thiotrophic bacteria in a hydrothermal vent mussel. Proc Natl Acad Sci USA 92:9598–9602
Felsenstein J (1985) Confidence-limits on phylogenies—an approach using bootstrap. Evol 39:783–791
Fisher CR, Brooks JM, Vodenichar J, Zande J, Childress JJ, Burke RA Jr (1993) The co-occurrence of methanotrophic and chemoautotrophic sulfur-oxidizing bacterial symbionts in a deep-sea mussel. Mar Ecol 14:277–289
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acid Symp Ser 41:95–98
Hiraishi A (1992) Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213
Kojima S, Ohta S, Yamamoto T, Yamaguchi T, Miura T, Fujiwara Y, Fujikura K, Hashimoto J (2003) Molecular taxonomy of vestimentiferans of the western Pacific, and their phylogenetic relationship to species of the eastern Pacific III. Alaysia-like vestimentiferans and relationships among families. Mar Biol 142:625–635
Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Maldonado M (2002) Family Pachastrellidae Carter, 1875. In: Hooper JNA, van Soest RWM (eds) Systema porifera. A guide to the classification of sponges vol. 1. Kluwer, New York, pp 141–162
McKiness ZP, McMullin ER, Fisher CR, Cavanaugh CM (2005) A new bathymodioline mussel symbiosis at the Juan de Fuca hydrothermal vents. Mar Biol 148:109–116
Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16S ribosomal-RNA. Appl Environ Microbiol 59:695–700
Schirmer A, Gadkari R, Reeves CD, Ibrahim F, DeLong EF, Hutchinson CR (2005) Metagenomic analysis reveals diverse polyketide synthetase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta. Appl Environ Microbiol 71:4840–4849
Siegl A, Bayer K, Kozytska S, Hentschel U, Schmitt S (2008) Sponges and microbes—new frontiers in an ancient symbiosis. Vie et Milieu-Life Environ 58:165–174
Sipkema D, Franssen MCR, Osinga R, Tranoer J, Wijffels RH (2005) Marine sponges as pharmacy. Mar Biotechnol 7:142–162
Stewart FJ, Newton ILG, Cavanaugh CM (2005) Chemosynthetic endosymbioses: adaptations to oxic–anoxic interfaces. Trends Microbiol 13:439–448
Sunamura M, Higashi Y, Miyako C, Ishibashi J, Maruyama A (2004) Two bacteria phylotypes are predominant in the Suiyo Seamount hydrothermal plume. Appl Environ Microbiol 70:1190–1198
Taylor MW, Radax R, Steger D, Wagner M (2007) Sponge-associated microorganisms: evolution, ecology and biotechnological potential. Microbiol Mol Biol Rev 71:295–347
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal-W. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acid Res 22:4673–4680
Uchino Y, Yokota A, Sugiyama J (1997) Phylogenetic position of the marine subdivision of Agrobacterium species based on 16S rRNA sequence analysis. J Gen Appl Microbiol 43:243–247
Vacelet J, Boury-Esnault N, Filamedioni A, Fisher CR (1995) A methanotrophic carnivorous sponge. Nature 377:296–296
Vacelet J, Boury-Esnault N (2002) A new species of carnivorous deep-sea sponge (Demospongiae, Cladorhizidae) associated with methanotrophic bacteria. Cah Biol Mar 43:141–148
Vacelet J, Fialamedioni A, Fisher CR, Boury-Esnault N (1996) Symbiosis between methane-oxidizing bacteria and a deep-sea carnivorous cladorhizid sponge. Mar Ecol Prog Ser 145:77–85
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Willenz P, Hartman WD (1989) Micromorphology and ultrastructure of Caribbean sclerosponges. I. Ceratoporella nicholsoni and Stromatospongia norae (Ceratoporellidae Porifera). Mar Biol 103:387–402