Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system

Microchimica Acta - Tập 185 - Trang 1-7 - 2017
Gang Liang1,2,3,4, Yan Man1,2,3, An Li1,2,3, Xinxin Jin1,2,3, Ligang Pan1,2,3, Xinhui Liu4
1Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Science, Beijing, People’s Republic of China
2Ministry of Agriculture, Risk Assessment Lab for Agro-products (Beijing), Beijing, People’s Republic of China
3Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Beijing, People’s Republic of China
4State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, People’s Republic of China

Tóm tắt

A chemiluminescence (CL) based assay is described for the determination of the environmental pollutant 2-hydroxyfluorene (2-HOFlu) which is found to inhibit the CL of a system composed of the G-quadruplex/hemin complex (a DNAzyme), H2O2, and luminol. The G-rich aptamer PW17 is transformed to a potassium(I)-stabilized G-quadruplex-hemin complex which displays peroxidase-like activity to catalyze the oxidation of luminol by H2O2 which is accompanied by strong blue CL emission. On addition of 2-HOFlu, it will participate in the G-quadruplex DNAzyme-mediated oxidation by H2O2. As a result, CL intensity is decreased. The difference in CL intensity (ΔI) before and after addition of 2-HOFlu serves as the signal for its quantitation. In water of pH 9.0, a linear relationship is found for the 1 nM to 1 μM concentration range, with a 0.2 nM detection limit. The assay is highly selective over other fluorene derivatives. It was successfully applied to the determination of 2-HOFlu in spiked lake water samples. The method is rapid, cost-effective and convenient. Conceivably, it has a wide scope in that it may be applied to other target pollutants for which G-quadruplexes are available.

Tài liệu tham khảo

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