The effect of chitosan–PMAA–NPK nanofertilizer on Pisum sativum plants

3 Biotech - Tập 8 - Trang 1-12 - 2018
Noha S. Khalifa1, Mohammed N. Hasaneen2
1Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt
2Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Tóm tắt

The use of chitosan (CS) as a carrier for slow fertilizer release is a novel trend. The potential effect of this system in agriculture is still debatable. Here, chitosan (CS) nanoparticles were obtained by polymerizing methacrylic acid (PMAA) for the entrapment of nitrogen, phosphorous and potassium (NPK) nanoparticles (NP), each at a time to form CS–PMAA–NPK NPs complex. The impact of this complex was evaluated using garden pea (Pisum sativum var. Master B) plants. Five-day-old pea seedlings were treated through their root system with CS–PMAA–NPK NPs at concentrations of 1, 0.5, 0.25, 0.125 and 0.0625 of the stock solution (R) for 1, 2, 4 and 7 days. In general, CS–PMAA–NPK NP complex reduced root elongation rate and resulted in the accumulation of starch at the root tip in a dose-dependent manner within the treated plants. Interestingly, the lowest concentrations of 0.0625 and 0.125 R had induced mitotic cell division (MI = 22.45 ± 2.68 and 19.72 ± 3.48, respectively) compared with the control (MI = 9.09 ± 3.28). In addition, some of major proteins such as convicilin, vicilin and legumin β were upregulated in plants treated with these low concentrations too. However, all concentrations used exhibited genotoxic effect on DNA based on the comet assay data after 48 h of treatment. Thus, it is highly recommended to consider the negative effects of this carrier system on plants and environment that may arise due to its accumulation in the agricultural fields.

Tài liệu tham khảo

Abdel-Aziz HMM, Hasaneen MNA, Omer AM (2016) Nano chitosan-NPK fertilizer enhances the growth and productivity of wheat plants grown in sandy soil. Span J Agric Res 14:902–911 Alfaro M, Salazar F, Iraira S, Teuber N, Villarroel D, Ramírez L (2008) Nitrogen, phosphorous and potassium losses in a grazing system with different stocking rates in a volcanic soil. Chili J Agric Res 68:146–155 Badawy MEI, Rabea E (2011) A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. Int J Carb Chem 2011:29 Barac M, Cabrilo S, Pesic M, Stanojevic S, Zilic S, Macej O (2010) Profile and functional properties of seed proteins from six pea (Pisum sativum) genotypes. Intern J Mol Sci 11:4973–4990 Barrenaa R, Casals E, Colóna J, Fonta X, Sáncheza A, Puntesbc V (2009) Evaluation of the ecotoxicity of model nanoparticles. Chemosphere 75:850–857 Baskar V, Venkatesh J, Park SW (2015) Impact of biologically synthesized silver nanoparticles on the growth and physiological responses in Brassica rapa ssp. pekinensis. Environ Sci Pollut Res 22:17672–17682 Beenken KE, Smith JK, Skinner RA, Mclaren SG, Bellamy W, Gruenwald MJ, Spencer HJ, Jennings JA, Haggard WO, Smeltzer MS (2014) Chitosan coating to enhance the therapeutic efficacy of calcium sulfate-based antibiotic therapy in the treatment of chronic osteomyelitis. J Biomater Appl 29(4):514–523 Cabaleiro-Lago C, Quinlan-Pluck F, Lynch I, Dawson KA, Linse S (2010) Dual effect of amino modified polystyrene nanoparticles on amyloid β protein fibrillation. ACS Chem Neurosci 1:279–287 Cedervall T, Lynch I, Lindman S, Berggård T, Thulin E, Nilsson H (2007) Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc Natl Acad Sci USA 104:2050–2055 Celis R, Adelino MA, Hermosín MC, Cornejo J (2012) Montmorillonite–chitosan bionanocomposites as adsorbents of the herbicide clopyralid in aqueous solution and soil/water suspensions. J Hazard Mater 210:67–76 Corradini E, de Moura MR, Mattoso LHC (2010) A preliminary study of the incorparation of NPK fertilizer into chitosan nanoparticles. Express Polym Lett 4:509–515 da Silva MA, Bode F, Drake AF, Goldoni S, Stevens MM, Dreiss CA (2014) Enzymatically cross-linked gelatin/chitosan hydrogels: tuning gel properties and cellular response. Macromol Biosci 14(6):817–830 DeRosa MC, Monreal C, Schnitzer M, Walsh R, Sultan Y (2010) Nanotechnology in fertilizers. Nat Nanotech 5:91 Di Bucchianico S, Cappellini F, Le Bihanic F, Zhang Y, Dreij K, Karlsson HL (2017) Genotoxicity of TiO2 nanoparticles assessed by mini-gel comet assay and micronucleus scoring with flow cytometry. Mutagen 32:127–137 Dimkpa CO, McLean JE, Martineau N, Britt DW, Haverkamp R, Anderson AJ (2013) Silver nanoparticles disrupt wheat (Triticum aestivum L.) growth in a sand matrix. Environ Sci Technol 47:1082–1090 FAO (2015) Food and Agriculture Organization of the United Nations, Rome, Italy. http://faostat.fao.org/default.aspx Ferraro D, Anselmi-Tamburini U, Tredici IG, Ricci V, Sommi P (2016) Overestimation of nanoparticles-induced DNA damage determined by the comet assay. Nanotoxicol 10:861–870 Gichner T, Patková Z, Száková J, Demnerová K (2006) Toxicity and DNA damage in tobacco and potato plants growing on soil polluted with heavy metals. Ecotox Environ Safe 65:420–426 Gichner T, Žnidar I, Száková J (2008) Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants. Mutat Res Genet Toxicol Environ Mutagen 652:186–190 Good AG, Beatty PH (2011) Fertilizing nature: a tragedy of excess in the commons. PLoS Biol 9(8):e1001124 Gritsch L, Wolfgang CL, Goldmann AH, Boccaccini R (2018) Fabrication and characterization of copper(II)-chitosan complexes as antibiotic-free antibacterial biomaterial. Carbohydr Polym 179:370–378 Hadwiger LA (2013) Multiple effects of chitosan on plant systems: solid science or hype. Plant Sci 208:42–49 Halling-Sorensen B, Jorgensen SE (1993) The removal of nitrogen compound in waste water. Elsevier, Amsterdam Hasaneen MNA, Abdel-Aziz HMM, El-Bialy DMA, Omer AM (2014) Preparation of chitosan nanoparticles for loading with NPK fertilizer. Afr J Biotech 13:3158–3164 Hossain Z, Mustafa G, Komatsu S (2015) Plant responses to nanoparticle stress. Intern J Mol Sci 16:26644–26653 Iriti M, Faoro F (2008) Abscisic acid mediates the chitosan-induced resistance in plant against viral disease. Plant Physiol Biochem 46:1106–1111 Janes KA, Calvo P, Alonso MJ (2001) Polysaccharide colloidal particles as delivery systems for macromolecules. Adv Drug Deliv Rev 47:83–97 Jiao X, Maimaitiyiming A, Salahou M, Liu K, Guo W (2017) Impact of groundwater level on nitrate nitrogen accumulation in the vadose zone beneath a cotton field. Water 9:171 Joseph T, Morrison M (2006) Nanotechnology in agriculture and food: a nanoforum report, Institute of Nanotechnology May 2006, www.nanoforum.org Kashyap PL, Xiang X, Heiden P (2015) Chitosan nanoparticle based delivery systems for sustainable agriculture. Int J Biol Macromol 77:36–51 Khalifa NS (2012) Protein expression after NaCl treatment in two tomato cultivars differing in salt tolerance. Acta Biol Crac Bot 54:79–86 Khati P, Chaudhary P, Gangola S, Bhatt P, Sharma A (2017) Nanochitosan supports growth of Zea mays and also maintains soil health following growth. 3 Biotech 7:81 Klosterman SJ, Choi JJ, Hadwiger LA (2003) Analysis of pea HMG-I/Y expression suggests a role in defense gene regulation. Mol Plant Path 4:249–258 Koppen G, Cerda H (1997) Identification of low-dose irradiated seeds using the neutral comet assay. LWT Food Sci Technol 30:452–457 Kumar G, Smith PJ, Payne GF (1999) Enzymatic grafting of a natural product onto chitosan to confer water solubility under basic conditions. Biotechnol Bioeng 63(2):154–165 Kumari M, Mukherjee A, Chandrasekaran N (2009) Genotoxicity of silver nanoparticles in Allium cepa. Sci Total Environ 15(19):2456–5243 Kuzma J, Verhage P (2006) Nanotechnology in agriculture and food production: anticipated application, 4th edn. Woodrow Wilson international center for scholars, Washington, DC, pp 1–40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 Li YZ, Zhao JY, Wu SM, Fan XW, Luo XL, Chen BS (2016) Characters related to higher starch accumulation in cassava storage roots. Sci Rep 6:19823 Lizardi-Mendoza J, Argüelles Monal WM, Goycoolea Valencia FM (2016) Chemical characteristics and functional properties of chitosan. Chitosan in the preservation of agricultural commodities. Elsevier, Amsterdam, pp 3–31 Malerba M, Cerana R (2016) Chitosan effects on plant systems. Inter J Mol Sci 17(7):996 Malerba M, Crosti P, Cerana R (2012) Defense/stress responses activated by chitosan in sycamore cultured cells. Protoplasma 249:89–98 Mondal MMA, Malek MA, Puteh AB, Ismail MR, Ashrafuzzaman M, Naher L (2012) Effect of foliar application of chitosan on growth and yield in okra. Aust J Crop Sci 6:918–921 Morales-Díaz AB, Ortega-Ortiz H, Juarez-Maldonado A, Cadenas-Pliego G, Gonzales-Morales S, Benavides-Mendoza A (2017) Application of nanoelements in plant nutrition and its impact in ecosystems. Adv Nat Sci 8:013001 Nagaonkar D, Shende S, Rai M (2015) Biosynthesis of copper nanoparticles and its effect on actively dividing cells of mitosis in Allium cepa. Biotech Prog 31:557–565 Nair PMG, Chung IM (2017) Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings. 3 Biotech 7:293 Nandhakumar S, Parasuraman S, Shanmugam MM, Rao KR, Chand P, Bhat BV (2011) Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay). J pharm pharmacother 2:107–111 Niki T, Gladish DK (2001) Changes in growth and structure of pea primary roots (Pisum sativum L. cv. Alaska) as a result of sudden flooding. Plant Cell Physiol 42:694–702 Nikolova I, Georgieva M, Stoilov L, Todorova D (2013) Zornica Katerova optimization of neutral comet assay for studying DNA double-strand breaks in pea and wheat. J Bio Sci Biotech 2:151–157 O’Neill A, Sen Gupta B, Phillips DH (2014) Distribution of arsenic and risk assessment of activities on a golf course fertilized with arsenic-containing Ascophyllum nodosum seaweed. Sci Total Environ 483:252–259 Osipov A, Arkhangelskaya E, Vinokurov A, Smetanina N, Zhavoronkov A, Klokov D (2014) DNA comet Giemsa staining for conventional bright-field microscopy. Inter J Mol Sci 15:6086–6095 Pang Y, Qin A, Lin X, Yang L, Wang Q, Wang Z et al (2017) Biodegradable and biocompatible high elastic chitosan scaffold is cell-friendly both in vitro and in vivo. Oncotarget 8:35583–35591 Pereira AES, Sandoval-Herrera IE, Zavala-Betancourt SA, Oliveira HC, Ledezma-Pérez AS, Romero J et al (2017) γ-Polyglutamic acid/chitosan nanoparticles for the plant growth regulator gibberellic acid: characterization and evaluation of biological activity. Carbohydr Polym 157:1862–1873 Radhakrishnan Y, Gopal G, Lakshmanan CC, Nandakumar KS (2015) Chitosan nanoparticles for generating novel systems for better applications: a review. J Mol Gene Med S4:005 Rajkishore S (2013) Nanotoxicity at various trophic levels: a review. Biosafety of nanoparticles. Toxicology 8(3):975–982 Ray PC, Yu H, Fu PP (2009) Toxicity and environmental risks of nanomaterials: challenges and future needs. J Environ Sci Health Part C 27:1–35 Roco MC (2011) The long view of nanotechnology development: the National Nanotechnology Initiative at 10 years. J Nanopart Res 13(2):427–445 Rodriguez E, Azevedo R, Fernandes P, Santos C (2011) Cr(VI) induces DNA damage, cell cycle arrest and polyploidization: a flow cytometric and comet assay study in Pisum sativum. Chem Res Toxicol 24:1040–1047 Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9(7):671 Scott N, Chen H (2013) Nanoscale science and engineering for agriculture and food systems. Ind Biotech 9:17–18 Sedbrook JC, Chen R, Masson PH (1999) ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton. Proc Natl Acad Sci USA 96:1140–1145 Siddiqi KS, Husen A (2016) Engineered gold nanoparticles and plant adaptation potential. Nanoscale Res Lett 11:400 Sivamani E, DeLong RK, Qu R (2009) Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells. Plant Cell Rep 28:213–221 Vold IMN, Varum KM, Guibal E, Smidsrød O (2003) Binding of ions to chitosan—selectivity studies. Carbohydr Polym 54:471–477 Wang L, Hua D, He J, Duan Y, Chen Z, Hong X et al (2011) Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in arabidopsis. PLoS Gen 7:e1002172 Wang Y, Xu C, Du LQ, Cao J, Liu JX, Su X et al (2013) Evaluation of the comet assay for assessing the dose-response relationship of DNA damage induced by ionizing radiation. Int J Mol Sci 14:22449–22461 White PJ, Brown PH (2010) Plant nutrition for sustainable development and global health. Ann Bot 105:1073–1080 Yan XL, Dai TF, Jia LM (2018) Evaluation of the cumulative effect of drip irrigation and fertigation on productivity in a poplar plantation. Ann For Sci 75:5 Yazdani MR, Virolainen E, Conley K, Vahala R (2018) Chitosan–Zinc(II) complexes as a bio-sorbent for the adsorptive abatement of phosphate: mechanism of complexation and assessment of adsorption performance. Polym 10(1):25 Yin H, Zhao X, Bai X, Du Y (2010) Molecular cloning and characterization of a Brassica napus L. MAP kinase involved in oligochitosan-induced defense signaling. Plant Mol Biol Rep 28:292–301 Zaman M, Ahmad E, Qadeer A, Rabbani G, Khan RH (2014) Nanoparticles in relation to peptide and protein aggregation. Int J Nanomed 9:899–912 Zawaski C, Ma C, Strauss SH, French D, Meilan R, Busov VB (2012) PHOTOPERIOD RESPONSE 1 (PHOR1)-like genes regulate shoot/root growth, starch accumulation, and wood formation in Populus. J Exp Bot 63:5623–5634 Zhang Y (2003) Transcriptional regulation by histone ubiquitination and deubiquitination. Genes Dev 17:2733–2740 Zivic F, Grujovic N, Mitrovic S, Ahad IU, Brabazon D (2018) Characteristics and applications of silver nanoparticles. Commercialization of nanotechnologies—a case study approach. Springer, New York, pp 227–273 Zuppini A, Baldan B, Millioni R, Favaron F, Navazio L, Mariani P (2003) Chitosan induces Ca2+‐mediated programmed cell death in soybean cells. New phytol 161(2):557–568