A multidrug resistance transporter from human MCF-7 breast cancer cells

Proceedings of the National Academy of Sciences of the United States of America - Tập 95 Số 26 - Trang 15665-15670 - 1998
Martin J. Edelman1, Weidong Yang1, Lynne V. Abruzzo1,2, Tammy Krogmann1, Yongming Gao1, Arun K. Rishi1, Douglas D. Ross3,1
1Greenebaum Cancer Center of the University of Maryland, Baltimore MD 21201; Department of Medicine, Division of Hematology/Oncology, University of Maryland School of Medicine, Baltimore, MD 21201; Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201; and Baltimore Veterans Medical Center, Department of Veterans Affairs, Baltimore, MD, 21201
2Hematopathology
3Baltimore VA Medical Center

Tóm tắt

MCF-7/AdrVp is a multidrug-resistant human breast cancer subline that displays an ATP-dependent reduction in the intracellular accumulation of anthracycline anticancer drugs in the absence of overexpression of known multidrug resistance transporters such as P glycoprotein or the multidrug resistance protein. RNA fingerprinting led to the identification of a 2.4-kb mRNA that is overexpressed in MCF-7/AdrVp cells relative to parental MCF-7 cells. The mRNA encodes a 663-aa member of the ATP-binding cassette superfamily of transporters that we term breast cancer resistance protein (BCRP). Enforced expression of the full-length BCRP cDNA in MCF-7 breast cancer cells confers resistance to mitoxantrone, doxorubicin, and daunorubicin, reduces daunorubicin accumulation and retention, and causes an ATP-dependent enhancement of the efflux of rhodamine 123 in the cloned transfected cells. BCRP is a xenobiotic transporter that appears to play a major role in the multidrug resistance phenotype of MCF-7/AdrVp human breast cancer cells.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 95 Số 26

15665-15670

Thông tin tác giả