Protective effect of polygoni cuspidati radix and emodin on Vibrio vulnificus cytotoxicity and infection

Journal of Microbiology - 2008
Jong Ro Kim1,2, Dool-Ri Oh2,1, Mi Hye Cha1,2, Byoung Sik Pyo1, Joon Haeng Rhee3,2,4, Hyon E. Choy4,2, Won Keun Oh5, Young Ran Kim1,2
1Department of Oriental Medicine Materials, Dongshin University, Jeonnam, Republic of Korea
2Clinical Vaccine R&D Center, Chonnam National University Medical School, Gwangju, Republic of Korea
3National Research Laboratory of Molecular Microbial Pathogenesis, Chonnam National University Medical School, Gwangju, Republic of Korea
4Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical School, Gwangju, Republic of Korea
5College of Pharmacy, Chosun University, Gwangju, Republic of Korea

Tóm tắt

Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.

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Tài liệu tham khảo

Baker-Austin, C., J.V. McArthur, A.H. Lindell, M.S. Wright, R.C. Tuckfield, J. Gooch, L. Warner, J. Oliver, and R. Stepanauskas. 2008. Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus. Microb. Ecol. In press.

Dhakal, B.K., W. Lee, Y.R. Kim, H.E. Choy, J. Ahnn, and J.H. Rhee. 2006. Caenorhabditis elegans as a simple model host for Vibrio vulnificus infection. Biochem. Biophys. Res. Commun. 346, 751–757.

Ding, Y., L. Zhao, H. Mei, S.L. Zhang, Z.H. Huang, Y.Y. Duan, and P. Ye. 2008. Exploration of emodin to treat alpha-naph-thylisothiocyanate-induced cholestatic hepatitis via anti-inflammatory pathway. Eur. J. Pharmacol. 590, 377–386.

Gray, L.D. and A.S. Kreger. 1986. Detection of anti-Vibrio vulnificus cytolysin antibodies in sera from mice and a human surviving V. vulnificus disease. Infect. Immun. 51, 964–965.

Hatano, T., M. Kusuda, K. Inada, T.O. Ogawa, S. Shiota, T. Tsu-chiya, and T. Yoshida. 2005. Effects of tannins and related polyphenols on methicillin-resistant Staphylococcus aureus. Phytochemistry 66, 2047–2055.

Hsiang, C.Y. and T.Y. Ho. 2008. Emodin is a novel alkaline nuclease inhibitor that suppresses herpes simplex virus type 1 yields in cell cultures. Br. J. Pharmacol. 155, 227–235.

Huang, Z., G. Chen, and P. Shi. 2008. Emodin-induced apoptosis in human breast cancer BCap-37 cells through the mitochon-drial signaling pathway. Arch. Pharm. Res. 31, 742–748.

Kim, Y.R., S.E. Lee, C.M. Kim, S.Y. Kim, E.K. Shin, D.H. Shin, S.S. Chung, H.E. Choy, A. Progulske-Fox, J.D. Hillman, M. Handfield, and J.H. Rhee. 2003. Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect. Immun. 71, 5461–5471.

Kim, Y.R., S.E. Lee, H. Kook, J.A. Yeom, H.S. Na, S.Y. Kim, S.S. Chung, H.E. Choy, and J.H. Rhee. 2008. Vibrio vulnificus RTX toxin kills host cells only after contact of the bacteria with host cells. Cell. Microbiol. 10, 848–862.

Kim, Y.R. and J.H. Rhee. 2003. Flagellar basal body flg operon as a virulence determinant of Vibrio vulnificus. Biochem. Biophys. Res. Commun. 304, 405–410.

Kothary, M.H. and A.S. Kreger. 1987. Purification and characterization of an elastolytic protease of Vibrio vulnificus. J. Gen. Microbiol. 133, 1783–1791.

Kreger, A. and D. Lockwood. 1981. Detection of extracellular tox-in(s) produced by Vibrio vulnificus. Infect. Immun. 33, 583–590.

Kumar, A., S. Dhawan, and B.B. Aggarwal. 1998. Emodin (3-methyl-1,6,8-trihydroxyanthraquinone) inhibits TNF-induced NF-kappaB activation, IkappaB degradation, and expression of cell surface adhesion proteins in human vascular endothelial cells. Oncogene 17, 913–918.

Lee, J.H., M.W. Kim, B.S. Kim, S.M. Kim, B.C. Lee, T.S. Kim, and S.H. Choi. 2007. Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice. J. Microbiol. 45, 146–152.

Lim, B.O., J.H. Lee, N.Y. Ko, S.H. Mun, J.W. Kim, D.K. Kim, J.D. Kim, B.K. Kim, H.S. Kim, E. Her, H.Y. Lee, and W.S. Choi. 2007. Polygoni cuspidati radix inhibits the activation of Syk kinase in mast cells for antiallergic activity. Exp. Biol. Med. (Maywood) 232, 1425–1431.

Lin, C.W., C.F. Wu, N.W. Hsiao, C.Y. Chang, S.W. Li, L. Wan, Y.J. Lin, and W.Y. Lin. 2008. Aloe-emodin is an interferon-induc-ing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71. Int. J. Antimicrob. Agents 32, 355–359.

Miyoshi, N., C. Shimizu, S. Miyoshi, and S. Shinoda. 1987. Purification and characterization of Vibrio vulnificus protease. Microbiol. Immunol. 31, 13–25.

Oliver, J.D. 2005. Wound infections caused by Vibrio vulnificus and other marine bacteria. Epidemiol. Infect. 133, 383–391.

Radu, S., N. Elhadi, Z. Hassan, G. Rusul, S. Lihan, N. Fifadara, Yuherman, and E. Purwati. 1998. Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis. FEMS Microbiol. Lett. 165, 139–143.

Reddy, G.P., U. Hayat, C. Abeygunawardana, C. Fox, A.C. Wright, D.R. Maneval, Jr., C.A. Bush, and J.G. Morris, Jr. 1992. Purification and determination of the structure of capsular polysaccharide of Vibrio vulnificus M06-24. J. Bacteriol. 174, 2620–2630.

Shuangsuo, D., Z. Zhengguo, C. Yunru, Z. Xin, W. Baofeng, Y. Lichao, and C. Yan’an. 2006. Inhibition of the replication of hepatitis B virus in vitro by emodin. Med. Set Monit. 12, BR302–306.

Simpson, L.M. and J.D. Oliver. 1983. Siderophore production by Vibrio vulnificus. Infect. Immun. 41, 644–649.

Tacket, C.O., F. Brenner, and P.A. Blake. 1984. Clinical features and an epidemiological study of Vibrio vulnificus infections. J. Infect. Dis. 149, 558–561.

Testa, J., L.W. Daniel, and A.S. Kreger. 1984. Extracellular phos-pholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect. Immun. 45, 458–463.

Wang, Z., Z. Cheng, and X. Fang. 1999. Antiviral action of combined use of rhizoma Polygoni cuspidati and radix Astragali on HSV-1 strain. Zhongguo Zhong Yao Za Zhi. 24, 176–180, 192.

Wang, H.H. and J.G. Chung. 1997. Emodin-induced inhibition of growth and DNA damage in the Helicobacter pylori. Curr. Microbiol. 35, 262–266.

Wright, A.C., L.M. Simpson, J.D. Oliver, and J.G. Morris, Jr. 1990. Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect. Immun. 58, 1769–1773.

Yi, T., H. Zhang, and Z. Cai. 2007. Analysis of rhizoma polygoni cuspidati by HPLC and HPLC-ESI/MS. Phytochem. Anal. 18, 387–392.

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Journal of Microbiology

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