3,3′,4,4′‐Tetrachlorobiphenyl Effects on Antioxidant Enzymes and Glutathione Status in Different Tissues of Rainbow Trout

Wiley - Tập 77 Số 4 - Trang 281-287 - 1995
Diana M. E. Otto1, Thomas W. Moon2
1Ottawa-Carleton Institute of Biology, Department Biology, University of Ottawa, Ontario, Canada.
2Ottawa-Carleton Institute of Biology, Department Biology, University of Ottawa, Ottawa, Ontario, K1N 6N5 Canada

Tóm tắt

Abstract: Polychlorinated biphenyls are known to cause induction in cytochrome P450‐dependent monooxygenase activities and alteration in the antioxidant defense of mammals. To determine whether similar detoxication processes are activated in rainbow trout (Oncorhynchus mykiss), we investigated P450‐dependent enzyme activities, antioxidant enzymes and glutathione status (reduced and oxidized glutathione, GSH and GSSG) in this species injected intraperitoneally with 3,3′,4,4′‐tetrachlorobiphenyl at 5 mg/kg body weight 6 weeks post injection. Ethoxyresorufin O‐deethylase activities increased 11‐ and 40‐fold in liver and kidney, UDPglucuronosyltransferase activities were 2‐ and 5‐fold higher in these organs, while glutathione S‐transferase activity was enhanced greater than 2‐fold in liver of tetrachlorobiphenyl injected trout in comparison with controls. Glutathione peroxidase activities were increased in liver and white muscle of dosed fish. Tetrachlorobiphenyl exposure resulted in a significant increase in glutathione reductase activities, with 7‐fold enhancement in liver and significantly elevated activities in kidney, red and white muscles. Similarly, cytosolic superoxide dismutase and catalase activities were increased in white muscle of injected trout. Tetrachlorobiphenyl exposure significantly increased GSH concentrations in liver and kidney, while GSSG levels were increased in liver and blood plasma. These changes, however, did not modify the GSSG/GSH ratios in these tissues. Overall, these results imply a major tetrachlorobiphenyl effect on GSH status and antioxidant enzymes in trout tissues and identify white muscle along with liver and kidney as important tissues in the detoxication process in this animal.

Từ khóa


Tài liệu tham khảo

10.1016/0041-008X(83)90096-0

10.1016/0003-2697(71)90370-8

Burke M. D., 1974, Ethoxyresorufin: direct fluorometric assay of a microsomal O‐dealkylation which is preferentially inducible by 3‐methylchloanthrene, Drug Metab. Disp., 2, 583

10.1016/S0076-6879(85)13062-4

10.1016/0041-008X(92)90131-B

10.1016/0166-445X(91)90040-G

10.1016/0003-2697(70)90083-7

10.1016/0163-7258(91)90029-L

Deneke S. M., 1989, Regulation of cellular glutathione, Amer. J. Physiol., 257, L163

10.1016/0014-5793(92)81276-R

10.1086/physzool.64.1.30158523

10.1016/0041-008X(89)90171-3

10.1016/0003-2697(80)90139-6

Habig W. H., 1974, Glutathione S‐tranferases. The first enzymatic step in mercapturic acid formation, J. Biol. Chem., 25, 7130

10.1096/fasebj.6.9.1612291

Hermes‐Lima M., 1993, Antioxidant defenses in the tolerance of freezing and anoxia by garter snakes, Amer. J. Physiol., 265, R646

Hänninen O., 1968, On the metabolic regulation in the glucuronic acid pathway in the rat tissues, Ann. Acad. Sci. Fenn. A2, 142, 1

10.1038/ki.1994.1

10.1016/0013-9351(84)90071-9

10.1016/0006-2952(86)90544-7

10.1016/0891-5849(94)90245-3

Kleinow K. M., 1987, Biotransformation and induction: Implication for toxicity, bioaccumulation and monitoring of environmental xenobiotics in fish, Environ. Health Persp., 71, 105, 10.1289/ehp.8771105

10.1002/jez.1402270106

10.1016/S0021-9258(19)52451-6

10.1006/eesa.1994.1021

10.1093/carcin/14.2.211

10.1016/0006-2952(86)90545-9

Rudneva‐Titova I. I., 1994, Effects of polychlorinated biphenyls on the activity of antioxidant enzymes and lipid peroxidation in muscle and liver of two black sea fish species, Biochem. Moscow, 59, 25

10.3109/10408449409049308

10.1016/0005-2760(90)90245-S

10.1016/0006-2952(85)90239-4

10.1016/0006-2952(93)90052-X

10.1152/jappl.1992.73.4.1265

10.1111/j.1748-1716.1993.tb09527.x

10.1016/S0013-9351(86)80095-0

10.1016/S0076-6879(78)52055-7

10.1016/0006-2952(88)90798-8

10.1016/0166-445X(93)90052-3

10.1016/0166-445X(91)90033-6

10.3181/00379727-201-43494

Thông tin
Thông tin xuất bản

Wiley

Tập 77 Số 4

281-287

Thông tin tác giả