Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis

Analytical Biochemistry - Tập 340 Số 1 - Trang 24-34 - 2005
Paul Monis1, Steven Giglio1, Christopher P. Saint1
1Microbiology Unit, Australian Water Quality Centre, Private Mail Bag 3, Salisbury, SA 5108, Australia

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Tài liệu tham khảo

Heid, 1996, Real time quantitative PCR, Genome Res., 6, 986, 10.1101/gr.6.10.986

Piatek, 1998, Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis, Nat. Biotechnol., 16, 359, 10.1038/nbt0498-359

Chen, 1999, Homogeneous genotyping assays for single nucleotide polymorphisms with fluorescence resonance energy transfer detection, Genet. Anal., 14, 157, 10.1016/S1050-3862(98)00016-3

Solinas, 2001, Duplex Scorpion primers in SNP analysis and FRET applications, Nucleic Acids Res., 29, E96, 10.1093/nar/29.20.e96

Howell, 2002, iFRET: an improved fluorescence system for DNA-melting analysis, Genome Res., 12, 1401, 10.1101/gr.297202

Higuchi, 1992, Simultaneous amplification and detection of specific DNA sequences, Biotechnology (NY), 10, 413, 10.1038/nbt0492-413

Higuchi, 1993, Kinetic PCR analysis: real-time monitoring of DNA amplification reactions, Biotechnology (NY), 11, 1026, 10.1038/nbt0993-1026

Ishiguro, 1995, Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater, Anal. Biochem., 229, 207, 10.1006/abio.1995.1404

Wittwer, 1997, Continuous fluorescence monitoring of rapid cycle DNA amplification, Biotechniques, 22, 130, 10.2144/97221bi01

Ririe, 1997, Product differentiation by analysis of DNA melting curves during the polymerase chain reaction, Anal. Biochem., 245, 154, 10.1006/abio.1996.9916

Bengtsson, 2003, A new minor groove binding asymmetric cyanine reporter dye for real-time PCR, Nucleic Acids Res., 31, e45, 10.1093/nar/gng045

Wittwer, 2003, High-resolution genotyping by amplicon melting analysis using LCGreen, Clin. Chem., 49, 853, 10.1373/49.6.853

Jung, 2001, Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye, Anal. Biochem., 289, 292, 10.1006/abio.2000.4931

Karsai, 2002, Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression, Biotechniques, 32, 790, 10.2144/02324st05

Nath, 2000, Effects of ethidium bromide and SYBR Green I on different polymerase chain reaction systems, J. Biochem. Biophys. Methods, 42, 15, 10.1016/S0165-022X(99)00033-0

Woo, 1998, Identification of Leptospira inadai by continuous monitoring of fluorescence during rapid cycle PCR, Syst. Appl. Microbiol., 21, 89, 10.1016/S0723-2020(98)80011-8

Pietila, 2000, Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene, J. Clin. Microbiol., 38, 2756, 10.1128/JCM.38.7.2756-2759.2000

Mommert, 2001, Sensitive detection of Borrelia burgdorferi sensu lato DNA and differentiation of Borrelia species by LightCycler PCR, J. Clin. Microbiol., 39, 2663, 10.1128/JCM.39.7.2663-2667.2001

Rantakokko-Jalava, 2001, Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens, J. Clin. Microbiol., 39, 2904, 10.1128/JCM.39.8.2904-2910.2001

Tanriverdi, 2002, Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis, J. Clin. Microbiol., 40, 3237, 10.1128/JCM.40.9.3237-3244.2002

Xu, 2000, A rapid method for determining the G+C content of bacterial chromosomes by monitoring fluorescence intensity during DNA denaturation in a capillary tube, Int. J. Syst. Evol. Microbiol., 50, 1463, 10.1099/00207713-50-4-1463

Giglio, 2003, Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR, Nucleic Acids Res., 31, e136, 10.1093/nar/gng135

R.P. Haugland, S.T. Yue, P.J. Millard, B.L. Roth, 1995. USA Patent No. 5,436,134

Monis, 1999, Molecular systematics of the parasitic protozoan Giardia intestinalis, Mol. Biol. Evol., 16, 1135, 10.1093/oxfordjournals.molbev.a026204

Suzuki, 1996, Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR, Appl. Environ. Microbiol., 2, 625, 10.1128/AEM.62.2.625-630.1996

Zipper, 2004, Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications, Nucleic Acids Res., 32, e103, 10.1093/nar/gnh101

Sambrook, 1989

Paltridge, 1998, Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease, Theor. Appl. Genet., 96, 1170, 10.1007/s001220050853