Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors
Tóm tắt
Flavonoids have recently aroused considerable interest because of their broad pharmacological activity. In fact, flavonoids have been reported to have antiviral, antiallergic, antiplatelet, anti-inflammatory and antitumoral activities. The pharmacological properties of bioflavonoids have been ascribed both to the concomitant inhibition of enzymes involved in the production of free radicals and to their free-radical scavenging and iron chelating capacity. However the antioxidant capacity of bioflavonoids due to free-radical scavenging and/or to iron chelating is still controversial. In this study, we have investigated the free-radical scavenging capacity of bioflavonoids (rutin, catechin, and naringin). In addition, the effects of these polyphenols on xanthine oxidase activity, spontaneous lipid peroxidation, and DNA cleavage were investigated. The bioflavonoids under examination showed a dose-dependent free-radical scavenging effect, a significant inhibition of xanthine oxidase activity, and an antilipoperoxidative capacity. In addition, they showed a protective effect on DNA cleavage.
Tài liệu tham khảo
Bonina F, Saija A, Tomaino A, Lo Cascio R, Rapisarda P, Dederen JC. In vitro antioxidant activity and in vivo photo-protective effect of a red orange extract. Int J Cosmetic Sci. 1998;20:331-42.
Cao G, Sofic E, Prior RL. Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med. 1997;22:749-60.
Carotenuto A, Fattorusso E, Lanzotti V, Magno S, De Feo V, Cicala C. The flavonoids of Allium neapolitanum. Phytochemistry. 1997;44:949-57.
Chan SC, Chang YS, Wang JP, Chen SC, Kuo SC. Three new flavonoids and antiallergic, anti-inflammatory constituents from the heartwood of Dalbergia odorifera. Planta Med. 1998;64:153-8.
Cheong H, Ryu SY, Oak MH, Cheon SH, Yoo GS, Kim KM. Studies of structure activity relationship for the anti-allergic actions. Arch Pharm Res. 1998;21:478-80.
Cook NC, Samman S. Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem. 1996;7:66-76.
Cotelle N, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM. Antioxidant properties of hydroxy-flavones. Free Radic Biol Med. 1996;20:35-43.
Critchfield JW, Butera ST, Folks TM. Inhibition of HIV activation in latently infected cells by flavonoid compounds. AIDS Res Hum Retroviruses. 1996;12:39-46.
De Groot H, Rauen U. Tissue injury by reactive oxygen species and the protective effects of flavonoids. Fundam Clin Pharmacol. 1998;12:249-55.
Feig DI, Reid TM, Loeb LA. Reactive oxygen species in tumorigenesis. Cancer Res. 1994;54:1890-4.
Gassen M, Youdim MB. The potential role of iron chelators in the treatment of Parkinson's disease and related neurological disorders. Pharmacol Toxicol. 1997;80:159-66.
Good PF, Werner P, Hsu A, Olanow CW, Perl DP. Evidence of neuronal oxidative damage in Alzheimer's disease. Am J Pathol. 1996;149:21-8.
Halliwell B. Free radicals, antioxidants and human disease: curiosity, cause or consequence? Lancet. 1994;344:721-4.
Halliwell B, Gutteridge MC. Oxygen toxicity, oxygen radical, transition metals and disease. Biochem J. 1984;219:1-14.
Hertog MGL, Kromhout D, Aravanis C, et al. Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch Intern Med. 1995; 155:381-6.
Hertog MG, Sweetnam PM, Fehily AM, Elwood PC, Kromhout D. Antioxidant flavonols and ischemic heart disease in aWelsh population of men: the Caerphilly study. Am J Clin Nutr. 1997;65:1489-94.
Hoult JRS, Moroney MA, Payà M. Action of flavonoids and coumarins on lipoxygenase and cyclooxygenase. Methods Enzymol. 1994;234:443-55.
Laughton MJ, Evans PJ, Moroney MA, Hoult JRC, Halliwell B. Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives: relationship to antioxidant activity and to iron ion-reducing ability. Biochem Pharmachol. 1991;42:1673-81.
Lotito Sb, Fraga CG. (+)-Catechin prevents human plasma oxidation. Free Radic Biol Med. 1998;24:435-41.
Lowry OH, Rosebrough NJ, Farr AL, Randall RC. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.
Manach C, Morand C, Demigne C, Texier O, Regerat F, Rémésy C. Bioavailability of rutin and quercetin in rats. FEBS Lett. 1997;409:12-6.
Mora A, Paya M, Rìos JL, Alcaraz MJ. Structure-activity relationships of polymethoxyflavones and other flavonoids as inhibitors of non-enzymic lipid peroxidation. Biochem Pharmacol. 1990;40:793-7.
Morel I, Lescoat G, Cogrel P, et al. Antioxidant and iron-chelating activities of the flavonoids catechin, quercetin and diosmetin on iron-loaded rat hepatocyte cultures. Biochem Pharmacol. 1993;45:13-9.
Nakao M, Takio S, Ono K. Alkyl peroxyl radical-scavenging activities of catechins. Phytochemistry. 1998;49:1178-82.
Paganga G, Al-hashim H, Khodr H, et al. Mechanisms of antioxidant activities of quercetin and catechin. Redox Report. 1996;2:359-64.
Paoletti F, Aldinucci D, Mocalli A, Caparrini A. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 1986;154:536-41.
Ratty AK, Das NP. Effects of flavonoids on nonenzymatic lipid peroxidation: structure-activity relationship. Oncology. 1988;39:69-79.
Renaud S, de Lorgeril M. Wine, alcohol, platelets and French paradox for coronary heart disease. Lancet. 1992;339:1523-6.
Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Rad Biol Med. 1996;20:933-56.
Rimm EB, Katan MB, Ascherio A, Stamper MJ, Willet WC. Relation between intake of flavonoids and risk for coronary heart disease in male health professionals. Ann Intern Med. 1996;125:384-9.
Routledge MN, Wink DA, Keefer LK, Dipple A. DNA sequence change induced by two nitric oxide donor drugs in the sup F assay. Chem Res Toxicol. 1994;7:628-32.
Saija A, Scalese M, Lanza M, Marzullo D, Bonina F, Castelli F. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med. 1995;19:481-6.
Sanz MJ, Ferrandiz ML, Cejudo M, et al. Influence of a series of natural flavonoids on free radical generating systems and oxidative stress. Xenobiotica. 1994;24:689-99.
Stocks J, Dormandy TL. The autoxidation of human red cell lipids induced by hydrogen peroxide. Br J Haematol. 1971;20:95-111.
van Acker SABE, van den Berg DJ, Tromp MNJL, et al. Structural aspects of antioxidant activity of flavonoids. Free Radic Biol Med. 1996;20:331-42.
Wei H, Zhang X, Zhao J, Wang Z, Bickers D, Lebwohl M. Scavenging of hydrogen peroxide and inhibition of ultra-violet light-induced oxidative DNA damage by aqueous extracts from green and black teas. Free Rad Biol Med. 1999; 26:1427-35.
Wernes SW, Lucchesi R. Free radicals and ischemic tissue injury. TIPS. 1990;11:161-6.
Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J. 1996;313:17-29.
You KM, Jong HG, Kim HP. Inhibition of cyclooxygenase/ lipoxygenase from human platelets by polyhydroxylated/ methoxylated flavonoids isolated from medicinal plants. Arch Pharm Res. 1999;22:18-24.